观察

The toxicity and the osteogenous ability on the surface of PCHA: the toxicity was examined by coculture with PCHA to record growth of cells with MTT method. The osteogenous ability was investigated by observation of the formation and growth of cells on the surface of PCHA bone cement.

PCHA毒性检测和表面细胞活性的观察：将PCHA浸出液与骨髓基质细胞共同培养，通过MTT比色描记细胞生长曲线，快速判定制孔剂添加后PCHA的毒性反应；将PCHA制成标准试件，与骨髓基质细胞共同培养，观察细胞形态和生长活性。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标：倒置相差显微镜下观察骨髓间充质干细胞的生长状态，流式细胞仪检测细胞表面标志物的表达，采用碱性磷酸酶染色鉴定成骨能力，以油红O染色鉴定成脂能力，以甲苯胺蓝染色鉴定成软骨能力。

METHODS Segmental, 1 cm osteoperiosteal defects were produced in both radii of 12 rabbits. One side was covered with hydroxyapatite/polylactic acid membrane encapsulated as a tube. The contralateral side served as an untreated control. Healing courses were detected by radiographic and histologic examinations.

采用新西兰大白兔为实验对象，将兔桡骨制成1cm缺损，一侧用羟基磷灰石／聚乳酸膜包绕缺损，另一侧作为对照，通过X线片、大体观察及病理检查等观察两侧桡骨愈合情况。

MAIN OUTCOME MEASURES: Morphological changes in primary cultured and subcultured mouse BMSCs were measured. BMSC surface antigen of the second passage of mice (CD105, CD44, CD25, CD34) were determined using flow cytometry. The modified method was assessed to harvest the purity of stem cells. Activity of mouse BMSCs was identified using adipogenic and osteoplastic differentiation. The strength of fluorescent cells following multiple passage was observed.

主要观察指标：原代及传代小鼠骨髓间充质干细胞形态变化；对第2代小鼠骨髓间充质干细胞表面抗原，CD105，CD44，CD25，CD34进行流式细胞仪检测，评价改良方法获取干细胞纯度；通过成脂成骨分化，鉴定小鼠骨髓间充质干细胞的活性；观察多次传代后荧光细胞强度。

3PH was done in group M and T. Then the rats in B group and M group were injected normal saline via portal vein, and those in T group were injected with SHCs suspension. Four week later, the level of serous alanine aminotransferaseand glutamic oxalacetic transaminase were tested, and liver pathological changes observed. Frozen sections of T group were observed under fluorescent microscope.

将实验用同一近交系SD大鼠30只随机平均分成空白对照组、损伤造模组和SHCs移植组。B组腹腔注射生理盐水，M组、T组腹腔注射CCl4.24 h后戊巴比妥腹腔麻醉，消毒开腹，M、T组行2/3肝切，B、M组门静脉注入生理盐水，T组门静脉注入经膜荧光标记SHCs悬液。4周后，采血测血清谷丙转氨酶、谷草转氨酶，并取病理标本做冰冻切片荧光显微镜下观察及HE染色普通光镜下观察。

MethodTotal 100 children with capillary bronchitis were divided into control group(50 cases)and observation group(50 cases)randomly,the children in observation group adopted ultrasonic atomizing inhalation,while the children in control group adopted oxygenous atomizing inhalation.

将100例毛细支气管炎患儿，随机分为对照组(n=50)和观察组(n=50)。观察组采用超声雾化吸入；对照组采用氧气驱动雾化吸入。

On the basis of light microscopic observation the immunohistochemical examination of CK,TG,NSE,vimentin and calcitonin was used to observe 9 cases of oxyphilic cell adenoma of the thyroid.

观察9例甲状腺嗜酸细胞腺瘤，在光镜观察的基础上，用免疫组织化学方法检测细胞角蛋白、甲状腺球蛋白、神经元特异性烯醇化酶、波形蛋白和降钙素。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

1Cubic millimeter tissue sampling was fixed with the mix of 1% glutaraldehyde and 4% paraform in 4℃.

利用兔后腿软组织损伤、海水浸泡模型，分别于损伤后浸泡于海水中2小时，浸泡后分别进行各种低渗盐水冲洗，分别于干预处理后1h、3h、6h、8h、12h，在损伤周围0.5cm范围内的肌肉组织中取材，0.5cm3损伤组织用4%福尔马林固定，HE染色，光镜下观察损伤组织的细胞浸润情况，用免疫组化法检测受伤肌肉组织凋亡相关基因Caspase3表达情况；1mm3损伤组织用1%戊二醛一%多聚甲醛混合固定液(pH7.4 ) 4℃固定，电镜观察其细胞凋亡及细胞膜、线粒体等结构的变化；生化检测Na+，K+-ATPase活性，了解其组间的差别。

METHODS: Doppler tissue velocity mode was used and the tricuspid annulus movements of anterior, septal and posterior attachment points in 60 healthy people were detected from apical four chamber view (AP 4CV), parasternal four chamber view (PS 4CV) and apical right heart two chamber view (AP RH 2CV).

应用sequoia 512彩色电脑声像仪，探头型号3V2c，H 3.5 MHz，选用胸骨旁四腔切面（PS 4CV）、心尖四腔切面（AP 4CV）观察60名健康志愿者三尖瓣环前瓣及隔瓣附着点，心尖右心两腔切面（AP RH 2CV）观察三尖瓣环后瓣附着点。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。