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METHODS Acute incomplete ischemia was induced by ligaturing bilateral carotid arterise and cutting heads in mice, the survival time and asthmatic time were observed; the coagulation timewas measured by sheet glass method,and the blood viscosity was also assayed; the platelet aggregation induced by collagen were studied by turbdimetry in vitro.

采用结扎小鼠双侧颈总动脉造成急性不完全性脑缺血模型,观察平均存活时间;另取小鼠断颅后测定断颅喘息时间;采用玻片法,观察胡芦巴总皂苷对凝血时间的影响;采用比浊法;观察其对体外血小板聚集率的影响,并测定全血粘度。

The polymorphism traits were compose d of heterozygosity , number of allele, range of allele-length between the maximum and minimum and informative meioses from reference populat ion and the observed paternal heterozygosity , maximum proportion of ha lf informative mating, observed proportion of half informative mating and informative meioses from experimental population.

分析了62个微卫星在参考群中的杂合程度、等位基因数、等位基因范围和信息配子数与实验群中相应微卫星的父本观察杂合度、最大半同胞信息交配比例、观察半同胞信息交配比例和信息配子数的相关程度,其中父本观察杂合度与最大半同胞信息交配比例相关系数最高为0。

B、 In the laserablation study of composite materials composed of 〓 and ozonized〓 precipitate as well as metal fullerides 〓, endohedralmetallofullerenes of the type 〓 were formed in the positive ion channel. c、 Inthe laser ablation study of rhodium containing metal fulleride 〓, two sequencesof metallofullerenes were formed in both the positive and negative ion channels, with〓 and 〓.respectively.

在激光烧蚀金属富勒烯盐的实验中发现形成的金属富勒烯类型依赖于金属元素的性质、含量及金属富勒烯的制备方法:a、在〓的实验中观察到取代型金属富勒烯〓和〓正负离子的产生。b、在对含La和Y的富勒烯复合样品〓以及金属富勒烯盐〓的激光烧蚀过程中观察到金属内嵌富勒烯正离子团簇〓的产生。c、在〓的激光烧蚀实验中观察到两个系列金属富勒烯〓和〓的形成,在负离子通道中〓在正离子通道2n=48-56。

The choose that the method chooses course of Ⅱ of ~ of Ⅰ of ASA of 30 60 years old of above period operation patient, random cent is contrast group and observation group each 15, capacity balance is maintained in art, art Bi Da arrives unplug after providing a standard, contrast 10 ML of physiological saline of group vein inject, observation group vein injects 0.5 Mg/kg of Ai Siluo Er, note with drop of 0.002% monobel vein, when subsequently observation unplugs tubal period differs paragraph of systole pressing , diastolic pressure , heartbeat and heartbeat and systole control product .

方法选择30例60岁以上ASAⅠ~Ⅱ级的择期手术患者,随机分为对照组和观察组各15例,术中维持容量平衡,术毕达到拔管标准后,对照组静脉注射生理盐水10 mL,观察组静脉注射艾司洛尔0.5 mg/kg,并以0.002%硝酸甘油静脉滴注,随后观察拔管期不同时间段收缩压、舒张压、心率及心率和收缩压乘积的变化。

Furthermore, the interference fringes of vortex beam are experimental investigated as well, it is found that the experimental observations are well consistent with the theoretical analysis.

对干涉条纹进行观察发现,实验观察结果和理论结果基本上保持一致,可以通过观察涡旋光束的干涉条纹来判断涡旋光束的轨道角动量。

The transmission magic-mirror method was applied to visualize and record the whole process of growth and melting of transparent crystals taking place in high temperature up to 1000℃ For matching to three-dimensional observation, a set of special furnace and crucible was designed and the growing process can be observed in two perpendicular directions and recorded in situ by CCD camera simultaneously.

最近开发了三维光学实时观察法,应用透视魔镜法实时观察并记录透明晶体的生长和溶解过程,最高使用温度为1000℃配合三维观察,设计并制造了一套独特的生长炉和坩埚,有二组互相垂直的光路可同时实时观察晶体生长过程,并通过CCD摄像头进行同步记录。

The LD50 of acute toxicity to mice and chronic accumulated toxicity to bandicoots of crude and processed Radix Aristolochice were observated.Intestinal and myokinetic influence of normal and revulsive hyperactive gastrointestinal motility of mice induced by neostigmine were observated by giving aqueous extract and alcoholic extract of crude and processed Radix Aristolochice.Relieving pain and eliminating inflammation to mice also were observated .

采用小鼠急性毒性和大鼠蓄积性毒性方法观察青木香炮制前后对小鼠急性毒性LD50和对大鼠的慢性蓄积性毒性作用;观察了青木香炮制前后的水煎剂和醇提物对正常和新斯的明诱导的胃肠运动机能亢进小鼠肠肌运动的影响;并观察了对小鼠的镇痛和抗炎作用。

To answer these questions, in the present study, an attempt was made to examine:(1) the GABA responses in mechanically dissociated Vme neurons with attached synaptic boutons by using the whole cell patch clamp technique;(2) the change of expression of KCC2 and NKCC1 during the postnatal stage in the Vme by using the Western blotting technique;(3) the percentage of colocalization of PV with KCC2 or NKCC1 during the postnatal stage in the Vme by using the double immunofluorescence histochemical techniques.

基于此,本论文进行了如下研究:(1)运用机械分离带终扣的单细胞膜片钳技术对大鼠Vme神经元对GABA的反应进行观察;(2)运用Western blotting技术结合图像分析方法观察生后发育过程中Vme神经元内NKCC1和KCC2的表达变化;(3)运用免疫荧光组织化学双重标记技术对生后发育过程中Vme神经元内NKCC1和KCC2与PV之间的共存关系进行了系统观察

The lung was fixed in 10% formalin solution and embedded in paraffin for Haematoxyffin-eosin staining to observed the form of embolisms, Masson staining to assess collagen and fibrin/fibrinogen distribution and Toluidine blue staining was to observe mast cell distribution.

以10%甲醛固定肺组织,制成组织切片,行苏木精-伊红染色观察血栓的形态,Masson染色观察栓塞血管的胶原纤维分布,甲苯胺蓝染色观察血管栓塞处肥大细胞分布。

Methods We established the model of human hair follicle in vitro in Williams E serum-free medium. We can continuously observe morphologic change of hair follicle under an invert microscope. The length of hair follicle was measured by eyepiece micrometer and DNA synthesis rate of human hair follicle in vitro by measuring the rates of incorporation of 3H-TdR.Histology form was observed by Cryo-section and transmission electron microscope.

在Williams E无血清培养基中,建立离体人头皮毛囊培养模型;通过倒置显微镜观察毛囊的形态变化;目镜测微器测量毛囊长度;3H-TdR 掺入检测毛囊DNA合成率;组织学检测用冰冻切片光镜观察和电镜切片透射电镜观察

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