见后

The antibodies in plasma were also detected by indirect antiglobulin test. The result showed that levels of WBC,TB,DB,Glu and reticulocyte in six patients treated with β-lactam antibiotics were raised remarkably. DAT was strongly positive,but no RBC antibodies were detected in the plasma by IAT. The binding of red cells by monocytes or lymphocytes was great,and the hemolysis of red cells by alexin not appeared. After stopping above-mentioned β-lactam antibiotic administration,patient's WBC,TB,DB,and Glu levels returned to normal values.

结果发现：6例患者的临床治疗的药物都属于β内酰胺类；住院期间患者的WBC、TB、DB、Glu的检测结果出现异常，红细胞的DAT试验结果均为阳性，IAT试验结果均为阴性；将DAT阳性的红细胞进行补体结合实验时，结果为阴性；细胞涂片见部分红细胞表面有颗粒状物质附着，细胞培养见部分红细胞被白细胞识别、黏附；改用其它抗生素后，患者的上述化验指标恢复到正常值范围内，DAT试验结果转为阴性。

The result showed that levels of WBC,TB,DB,Glu and reticulocyte in six patients treated with β-lactam antibiotics were raised remarkably. DAT was strongly positive,but no RBC antibodies were detected in the plasma by IAT. The binding of red cells by monocytes or lymphocytes was great,and the hemolysis of red cells by alexin not appeared. After stopping above-mentioned β-lactam antibiotic administration,patient's WBC,TB,DB,and Glu levels returned to normal values.

结果发现：6例患者的临床治疗的药物都属于β内酰胺类；住院期间患者的WBC、TB、DB、Glu的检测结果出现异常，红细胞的DAT试验结果均为阳性，IAT试验结果均为阴性；将DAT阳性的红细胞进行补体结合实验时，结果为阴性；细胞涂片见部分红细胞表面有颗粒状物质附着，细胞培养见部分红细胞被白细胞识别、黏附；改用其它抗生素后，患者的上述化验指标恢复到正常值范围内，DAT试验结果转为阴性。

Stores the first key condition of a check, a coffee shop exterior image of the results: the grade must be in line with the coffee shop, bearing in mind the personality and the impression of the performance of the shape, but also to maintain a sense of beauty. 2, signs "visible results" of performance: For the "easy to see a high degree of" certainty, we should still be able to make it clear in the distance, and attention should be paid to the suitability of the specifications. 3, store window charm: the performance of windows to be commensurate with the style, while attractive, will have to have induced. 4, to facilitate the import入店: the first shop to have the appropriate degree of openness, and easy to see the coffee-house, at the same time be able to have intimacy. 5, the first shop in the entire facility: a good performance must be able to store the image of the situation in order to avoid fouling, and even to consider closing the store after the first performance.

、店头状况检核重点 1、咖啡店外装形象的效果：必须与咖啡店品位符合，同时注意个性的表现与印象的塑造，而且要保持美感。2、招牌"易见效果"的表现：对于"易见高度"的把握，要使它在远处仍可以一目了然，且要注意规格的适合性。3、店面橱窗的魅力：橱窗的表现要能与风格相称，同时要具有吸引力，进而要具有诱导性。4、便于入店的进口：要有适当的店头开放度，并且易于看到咖啡店内部，同时要能有亲切感。5、店头的整个设施：必须要能表现好的店面形象，避免有污损状况，甚至要考虑打烊后的店头表现。

A,stores the first key condition of a check,a coffee shop exterior image of the results: the grade must be in line with the coffee shop,bearing in mind the personality and the impression of the performance of the shape,but also to maintain a sense of beauty. 2,signs "visible results" of performance: for the "easy to see a high degree of" certainty,we should still be able to make it clear in the distance,and attention should be paid to the suitability of the specifications. 3,store window charm: the performance of windows to be commensurate with the style,while attractive,will have to have induced. 4,to facilitate the import入店: the first shop to have the appropriate degree of openness,and easy to see the coffee-house,at the same time be able to have intimacy. 5,the first shop in the entire facility: a good performance must be able to store the image of the situation in order to avoid fouling,and even to consider closing the store after the first performance.

尤其对于店面的房子装修预算，要透过定期与不不定期的检查与清点；至于检核的重点，可以分成店头状况、店内家居装修设计图、陈列装饰及管理设施等方面进行。a、店头状况检核重点 1、咖啡店外装设计图的效果：必须与咖啡店品位符合，同时注意个性的表现与印象的塑造，而且要保持美感。2、招牌"易见效果"的表现：对于"易见高度"的装修设计图，要使它在远处仍可以一目了然，且要注意规格的房屋装修预算书性。3、店面橱窗的魅力：橱窗的表现要能与风格相称，同时要具有吸引力，进而要具有诱导性。4、便于入店的进口：家居装修设计图适当的店头开放度，并且易于看到咖啡店内部，同时要能有亲切感。5、店头的装修设计图设施：必须要能表现好的店面形象，制作淘宝装修素材有污损状况，甚至要 qq空间免费装饰打烊后的店头表现。

In the experimental tectum, the expression of GFAP in the right SO was higher than the expression of GFAP in the left SO during 1d-60d after after optic nerve damage. The expression of GFAP in the right SO was familiar with the expression of GFAP in the left SO during 85d after optic nerve damage, but the expression of GFAP in SO in the experimental tectum was higher than that in the normal tectum. The result of GFAP expression in the experimental retina and tectum showed that AStrocyte took part in the optic nerve regeneration and it might play an important role in optic nerve regeneration accidents.

在损伤1d后视网膜的神经纤维层和外核层外缘可见较为深的GFAP染色，在损伤3d后视网膜从内网层经内核层至外网层出现稀疏的垂直于视网膜长轴分布的线条状GFAP染色，随时间推移，伤后5d、7d、14d、28d上述染色进一步加深密集，到伤后60d和85d上述染色减弱，阴性对照的视网膜各层未见GFAP阳性染色；(5)正常鲫鱼视顶盖中在SO层内有较浅的黄棕色线条状GFAP染色，在SFGC层

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达，16Hz、130dB次声作用1d组：两者在海马〓区和齿状回轻度表达，海马内血管轻度扩张；7d组：两者表达在上述区域较1d增多，微血管和小血管扩张较显，血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA；14d组：海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多，呈深兰色，在部分血管内皮细胞内两者强阳性表达，微小血管扩张状态较7d减轻；21d组：锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱；28d组：上述表达及分布与21d组相比无明显改变，海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达，血管扩张状态已明显减轻。90dB次声作用1d-7d后，较对照组无明显变化，14d-21d后轻度表达，28d后，Bcl-2mRNA和P53mRNA表达较强。

PCR,urethrascope or biopsy was applied to some patients.52cases were seminal vesiculitis,28cases were prostatitis,29cases were genital tuberculosis,26cases were adenoma of the posterior urethra,11cases were cyst of prostate and seminal vesicle,8cases were hemospermia of postintercourse,9cases were idiopathic.They were treated by medicine,operation and adjuvant ways respectively.5cases of epididymal tuberculosis underwent epididymectomy because of deficiency of medicine.2cases of adenoma of the posterior urethra after electrotomy recured,which underwent reoperation.

诊断 精囊炎52例，前列腺炎28例，生殖系统结核29例，后尿道腺瘤26例，前列腺和精囊囊肿11例，性交后血精8例，特发性血精9例，分别给予药物、手术或对症治疗。5例附睾结核药物治疗效果不佳而行附睾切除术，后尿道腺瘤行肿瘤电灼术后复发2例，再手术后短期未见复发。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P＞0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P＜0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P＜0.05).The levels of CGRP and SP in CSD group were higher than control group(P＜0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P＞0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P＜0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P＜0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P＜0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P＜0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD；同侧TNC放电频率，CSD中＞CSD后＞CSD前P＜0.05对侧TNC放电频率，CSD前、中、后无统计学差异(P＞0.05氟桂利嗪组同侧TNC放电频率，CSD前＞CSD中(P＜0.05)，CSD前与CSD后及CSD中与CSD后之间无统计学差异(P＞0.05)。2.1关于放免测定，各组血浆CGRP、SP水平有统计学差异(P＜0.05)，CSD组高于对照组(P＜0.05)，CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P＞0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P＞0.05.2关于免疫组化研究，右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P＜0.05)，多重两两比较结果CSD组大于对照组(P＜0.05)，CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P＞0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P＞0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P＜0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P＜0.05，蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P＜0.05，false discovery rate，FDR校正疼痛状态与非疼痛状态比较，同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P＜0.05，蒙特卡罗模拟校正。

METHODS: Acetic acid solution was added into chitosan to prepare 8% chitosan acetic acid hydrosol, which was then stayed for one day. Flowing the immersion into the hydrosol and immediate dislodgement, plastic tube was soaked in 1 mol/L sodium hydroxide for 3 minutes. Hydrosol out of the tube etiolated and coagulated into jells, the tube was removed when the jells were semi-dried.

利用乙酸溶液制备8%壳聚糖乙酸水溶胶，溶胶放置1 d，将硬塑料管浸入溶胶后迅速取出放入1 mol/L氢氧化钠液内3 min，见管外溶胶变白凝固成胶冻状，取出后晾到五分干后轻轻退下内管，晾干后即成为壳聚糖导管。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。