见后

RESULTS: The collapse was obvious at weeks 2 and 6 after modeling, with only few granulation tissues and fibrous tissues. No chondrocytes were observed. At weeks 12 and 18, fibrous tissues were found at the defect site, and bone tissue and cartilage proliferation was observed at the bottom and margin. However, it was greatly different from normal cartilage. Moreover, there was still no chondrocytes.

结果：实验兔后腿关节的股骨髁关节面的关节软骨缺损后，在制模后2，6周缺损部位凹陷明显，只有少许肉芽组织和纤维组织生长填充，未见软骨细胞爬行生长，12，18周见缺损部位以纤维组织增生为主，底部及边缘可见骨组织和软骨增生，但是镜下形态仍与正常软骨组织区别较大，表面仍未见软骨细胞爬行生长。

Celiac were much more than outer stomach in the part recurrence. The Ⅱ,Ⅲ,Ⅳ type were much more in Bormann classification. Low polarization tumors were much more. Part recurrence were less after complete gastrectomy except celiac recurrence. Cardia circumambience and liver-duodenum ligaments (No. 1, 12) lymph node were more in the outer stomach recurrence.

局部复发病例中以腹膜腔复发多见，胃外复发次之；Bormann分型以2、3、4型复发多见；低分化肿瘤复发多见，全胃切除后除腹膜腔复发外局部复发少；胃外复发病例中以贲门周围和肝十二指肠韧带即第No.1、12淋巴结复发多见。

On the membrane of liver parenchymal cell and endothelial cell ×12 000;Fig 4 3 h after Cd-injection:ER extremely dilatant,mitochondria swollen,a lot of Ce-H2O2 precipitation on the membrane of PC,EC and Kupffer＇scell ×15 000;Fig 5 6 h after Cd-injection:in irreversible state,hepato-cytoplasm clear,few organelle,a few Ce-H2O2 precipitation on the membrane of PC and eC ×5 500;Fig 6 24 h after Cd-injection:hepatocyte plasmotorrhexis,membrane of

ce呈点状沉积在肝实质细胞、内皮细胞膜上×12 000；图4 染镉后3小时：ER极度扩张，线粒体膨胀，内外膜模糊不清；见大量Ce沉积在PC、EC和Kupffer＇s细胞膜上×15 000；图5 染镉后6小时：处于不可逆改变状态的肝细胞胞浆清亮，少有细胞器；仍可见少量Ce沉积在PC和EC膜上×5 500；图6 染镉后24小时：可见破碎的肝细胞，胞内膜结构大多已不清晰；未见Ce的沉积×7 000

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

The induced hmscs-tert were examined by oil red-o staining, alkaline phosphates staining and von kossa staining. results the hmsc-tert was negative for cd34, cd45, cd44, cd106 and cd166. after the induction, accumulated cytoplasmatic red lipid droplets were clearly visible in the adipose cells by oil red o staining, and alkaline phosphates-positive pitchy grains formed in the intracytoplasm and calcium nodes were found by von kossa staining in the osteoblasts.

结果 hmsc-tert细胞表面抗原检测cd34、cd45、cd44、cd106、cd166均为阴性；脂肪细胞定向诱导后油红o染色见胞浆内有红色脂肪颗粒，成骨细胞定向诱导后见胞浆内有碱性磷酸酶染色阳性黑褐色颗粒，von kossa染色见有钙结节形成。

Results:(1)Among 40 rats, 36 rats were successfully established and the rate of success is 90 percent;(2)All the successfully established models demonstrated polydipsia, polyuria and the body weight was not increased. 6 rats suffered cataract after 3 months, and 4 rats died in 6 months;(3)There was an approximately 61% loss of retinal ganglion cells in the central retina and the thickness of retina thinner under microscope ( P 0.01 ).(4) Electroscope changes include the thinner of retina, disorganization of the membranous disc of the rod cells and the thickness of basal membrane of vessal.(5)In normal group, 1 month dibetes mellitus and 1 month treatmen group, there was no expression of ERK1/ERK2 on the retina tissues. In 3 month diabetes mellitus group, the expression of ERK1/ERK2 was positive.

结果：①40只建模大鼠中36只建模成功，建模成功率为90%；②建模成功的大鼠都表现出多饮、多尿、消瘦、体重不增的表现，有6只大鼠在3个月后出现白内障，有4只大鼠在喂养接近至6个月时死亡；③HE染色光镜下6个月大鼠后极部视网膜节细胞层细胞数明显减少，减少约61%(P.01)，后极部视网膜明显变薄(P.01)；④电镜观察，视网膜变薄，视杆细胞膜盘紊乱，血管基底膜增厚等表现；⑤正常组、糖尿病组和治疗组1个月大鼠视网膜中未见ERK1/ERK2的表达，糖尿病组视网膜组织中3个月时可见少量表达，ERK1/ERK2表达部位为神经节细胞层和内核层；6个月时表达强阳性，部位表达不仅见于内核层、神经节细胞层，色素上皮层也见表达。

In Group A,one sample was found blood current tenuity at 14 days,one was occlusive in the femoral vein at 28 days ,the blood current ran into the vena cava posterior through the branch stem.The others were normal .And in Group B,there were one sample in each phase obliterative in the femoral vein because of relapse . For scanning electron microscopy result,Group A had a process which the injury of endothelial cells were from lower-grade to repair.

结果：1。影像学检查DSA下行血管造影见C、D两组术后第7天、14天和28天股静脉血流通畅，无明显充盈缺损或闭塞，A组第14天造影1只家兔股静脉血管纤细，血流尚通畅，第28天造影见1只家兔股静脉闭塞，血流从侧枝进入后腔静脉，其余时相股静脉血流通畅无明显异常；B组第7、14、28天造影各见1只家兔股静脉闭塞，其余未见明显异常。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果：1、通过不同感染途径的实验证实，滴鼻感染的小鼠主要表现为间质性肺炎，而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现，随着感染Q热立克次体剂量的加大，动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现，在腹腔注射后第2d的脾和肝脏即可发现病原体，主要脏器的病理变化从第2d到第12d逐渐加重，第7d动物的病变最典型，至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统，在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内，呈多形性，多见圆形和杆状，大小不一。

The results were expressed in mean±1SD. Pearson X~2 test and One-way ANOVA test were used. The data analyzed using the SPSS (version 11.5). Results: The sensitivity, specificity, positive and negative value of US for the LPEH model on the children cadaver were 88%, 84%, 79%, 91%, respectively. With regard to the thickness of femora head cartilage, the thickness of the anterior layer or posterior layer, there were no significant differences among three groups. However, the anterior layer was thicker than the posterior layer in three groups. The fluid in hip joint was detected in all of 21 symptomatic hips, which was clear commonly (90%) in early procedure. The amount of fluid in anterior recess showed a positive correlation with age (p .05). No fluid was detected in the asymptomatic and normal hips (2mm). The mean maximum width of inferomedial recess was significantly larger than that of anterior recess (12.50±4.04mm vs.4.35±0.8mm, p 0.05) in the symptomatic hip joints. The echogenic entrapped labral plicaes were demonstrated in the inferomedial recess in all of 21 children with LPEH, whose length and width ranged from 5.3mm-25.0mm (mean,15.6±5.6mm) and from 4.0mm-17.0mm (mean,8.9±7.8mm).

结果1，尸体LPEH髋关节模型的超声诊断敏感性、特异性分别为88％、84％，阳性预测值、阴性预测值分别为79％、91％。2，21例患儿的LPEH患髋(21侧)、健髋(21侧)，以及21例正常儿童健髋(42侧)的超声检查显示：髋关节周围软组织及股骨头无形态结构差别；股骨头软骨厚度无统计学差异(3.5±0.5mm vs.3.6±0.4mm vs.3.6±0.5mm，p＞0.05)；关节囊前层及后层厚度无统计学差异(前层厚度2.79±0.74 mm vs.2.56±0.40mm vs.2.56±0.72mm；后层厚度2.70±0.82mm vs.2.48±0.54mm vs.2.44±0.58mm，p＞0.05)，但LPEH患髋关节囊前、后层均较后二组有增厚趋势。3，LPEH患髋均存在关节腔内积液，且早期较为清晰；积液以髋关节内下间隙明显，内下间隙较前间隙明显增宽(12.50±4.04mm vs.4.35±0.8mm，p＜0.05)，其内见嵌顿滑膜唇皱襞呈稍强回声的占位性团块，长约15.6±5.6mm，宽约8.9±7.8mm，90.5％(19／21)嵌顿皱襞内未见血流信号。4，所有LPEH患髋治疗后超声复诊均显示正常。

The results show that the water breakthrough time shortens with the increase of the horizontal well length. Under the same well space and production conditions, the water breakthrough time of five-spot pattern is longer than that of seven-spot and nine-spot pattern. With the increase of angle between the horizontal well and the horizontal direction, the water breakthrough time shortens in five-spot well pattern, firstly increases and then decreases in seven-spot pattern, and increases in nine-spot pattern. The influence of reservoir and fluid parameters on the water breakthough time is little.

计算结果表明，井网的见水时间随水平井长度的增加而缩短；相同的井距和生产条件下，五点法井网的见水时间要长于九点法和七点法井网；随水平井与水平方向夹角的增大，五点法井网的见水时间缩短，七点法井网的见水时间先增加后缩短，九点法井网的见水时间增加；油藏和流体参数对井网见水时间影响不大。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。