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Methods: Pig-spleen cells were cultured with HB-vaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator; the homogenate was diluted with distilled water to 1:1, then frozen and melt six times at -25℃ before placed in the dialyzer at the melting state, after that dialyzed with 1:4 distilled water as the out-dialyzate at 4℃ for 48 h; the final out-dialyzate was collected as the HBV-STF.

方法;采用猪脾细胞与植物血凝素、乙肝疫苗共同培养,收集致敏细胞在组织捣碎机中破碎,用蒸馏水1:1稀释,-25℃反复冻融6次,自来水融化后装入透析袋中,用1:4的蒸馏水作透析外液,于4℃透析48h,透析液即为乙肝病毒特异性转移因子。

The experimental process was as follows: a set of 5 immobilized copper ion affinity membranes were loaded into a column, uncombined copper ion was removed with 5 mL of sodium phosphate-citric acid buffer (pH 7); the Hb sample volume was 2 mL, and uncombined Hb was eluted with 10 mL of the buffer.

中文内容:摘要:将纤维素滤纸进行碱处理及环氧活化、偶联亚氨基二乙酸、固定化铜离子等处理,并将其装入自制的色谱柱管,制得固定化铜离子亲和膜色谱柱。

I plan to mount the gear box as close to the back of the WTC as possible.

我计划在装入齿轮箱接近背部的世台会尽可能。

SPE method: condition SAX cartridges with 2 ml MeOH and 4 ml water, load 1 ml sample, wash with 3 x 1 ml water, elute with 2 ml

固相萃取方法:用2毫升甲醇和4毫升水洗涤SAX 柱,装入1毫升样品,1毫升的水洗三次,2毫升氯化钠(0.5M)溶液洗提。

It can be used on cleaning SO〓 of indoor air as filter materials of air cleaning machine or garnishry.

装入空气净化器或作为装饰品可较为有效地净化室内空气中的微量二氧化硫。

The data of element analysis,melting point,IR and NMR for the product are consistent with the standard data,the purity exceeds 98%.3-methyl-2-oxobutanoic acid sodium ; sodium methoxide ; isobutyraldehyde ; diethyl oxalate

WSR-1熔点仪;Vario EL元素分析仪(德国E le-mentar公司);Nexus 870型傅立叶变换红外光谱仪;aV500核磁共振仪。1.2 3-甲基-2-氧代丁酸钠的制备将15.4 g(0.28 mol)甲醇钠与60 mL(1.5 mol)甲醇装入500 m

Not a problem if you're strolling sockless in a pair of sandals. Big problem if all of those sweat glands are swaddled in a sock and encased in a leather boot.

如果你不穿袜子只穿着凉鞋闲逛那就没有什么问题,如果所有这些汗腺都被袜子包起来并且被装入一个皮的靴子,那就是个大问题了。

Tapping screws may be tested using pre-threaded holes in test plates to achieve screw failures instead of thread strippage.

自攻螺钉应装入带有螺纹孔的试验圆板上拧至螺钉损坏,而不是在螺纹测试条上进行。

Withdraw the plunger as far as possible by unscrewing anti-clockwise to the maximum extent.

把活塞装入带卡口的杯体中,之后按逆时针方向松开卡口底座螺丝将活塞经可能地拔出到最大体积。

To determine where that position is, load and clamp unwelded parts into the machine.

在合适的位置,装入并夹紧待焊部件。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。