装入因子

Methods:Pigspleen cells were cultured with HBvaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator;the homogenate was diluted with distilled water to 1∶1, then frozen and melt six times at25 ℃ before placed in the dialyzer at the melting state , after that dialyzed with 1∶4 distilled water as the outdialyzate at 4 ℃ for 48 h;the final outdialyzate was collected as the HBVSTF.

采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1∶1稀释，－25 ℃反复冻融6次，自来水融化后装入透析袋中，用1∶4的蒸馏水作透析外液，于4 ℃透析48 h，透析液即为乙肝病毒特异性转移因子。

Methods: Pig-spleen cells were cultured with HB-vaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator; the homogenate was diluted with distilled water to 1:1, then frozen and melt six times at -25℃ before placed in the dialyzer at the melting state, after that dialyzed with 1:4 distilled water as the out-dialyzate at 4℃ for 48 h; the final out-dialyzate was collected as the HBV-STF.

方法；采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1：1稀释，-25℃反复冻融6次，自来水融化后装入透析袋中，用1：4的蒸馏水作透析外液，于4℃透析48h，透析液即为乙肝病毒特异性转移因子。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。