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Methods:Pigspleen cells were cultured with HBvaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator;the homogenate was diluted with distilled water to 1∶1, then frozen and melt six times at25 ℃ before placed in the dialyzer at the melting state , after that dialyzed with 1∶4 distilled water as the outdialyzate at 4 ℃ for 48 h;the final outdialyzate was collected as the HBVSTF.

采用猪脾细胞与植物血凝素、乙肝疫苗共同培养,收集致敏细胞在组织捣碎机中破碎,用蒸馏水1∶1稀释,-25 ℃反复冻融6次,自来水融化后装入透析袋中,用1∶4的蒸馏水作透析外液,于4 ℃透析48 h,透析液即为乙肝病毒特异性转移因子。

Methods: Pig-spleen cells were cultured with HB-vaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator; the homogenate was diluted with distilled water to 1:1, then frozen and melt six times at -25℃ before placed in the dialyzer at the melting state, after that dialyzed with 1:4 distilled water as the out-dialyzate at 4℃ for 48 h; the final out-dialyzate was collected as the HBV-STF.

方法;采用猪脾细胞与植物血凝素、乙肝疫苗共同培养,收集致敏细胞在组织捣碎机中破碎,用蒸馏水1:1稀释,-25℃反复冻融6次,自来水融化后装入透析袋中,用1:4的蒸馏水作透析外液,于4℃透析48h,透析液即为乙肝病毒特异性转移因子。

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