裂细胞

Fluorescence in situ hybridization was performed to detect the existence of HBV DNA in the blastomere by using the biotinylated probe.

废弃的胚胎卵裂球经激光打孔，活检2～4个卵裂球细胞，利用生物素标记的探针，应用荧光原位杂交技术检测HBV感染情况。

The mitotic error rate in cleavage stage embryos was proven to be higher than the meiotic aneuploidy rate and as a consequence, the genome of a single blastomere is not representative for the genome of the other cells of the embryo.

在卵裂期胚胎这种有丝分裂的错误率被证明是高于减数分裂的非整倍体错误率，因此，单个卵裂球的基因不能代表其他胚胎细胞的基因组。

The cells of the blastula called blastomeres also each receive a full diploid set of chromosomes.

这些囊胚细胞，称卵裂球，每个细胞都有一套二倍体染色体组。

The study of the histopathology showed that the haemolymph in the disease crab decreased sharply, and was replaced by large amount of parasites, which was observed in muscle, heart and hepatopancreas from the disease crab. The pathological characteristics mainly appeared as cells swelling, necrosis, nucleus condensation and collaspe.

组织病理学观察发现，病蟹血淋巴急剧下降，代之以大量的寄生原虫，该寄生虫在病蟹的肝胰腺、鳃、心脏、肌肉等部位大量侵袭，并引起这些组织发生以坏死为主的变质性病变，其病理特征主要表现为：细胞肿胀、变性、坏死，某些细胞的细胞核固缩、碎裂或崩解。

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内，隐孢子虫出现连续发育阶段，包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊，在60～72 h内形成卵囊；用感染48 h的细胞培养上清接种于免疫抑制小鼠， 10 d后有隐孢子虫卵囊排出。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球为供体克隆小鼠的研究该研究以C57BL/6 小鼠致密桑椹胚卵裂球为供体、昆明白小鼠MⅡ期卵母细胞为受体，研究了不同蔗糖浓度对核移植操作时间及重构胚体外发育的影响。

Results: The differentiated neuron appeared different patterns of amitotic figures such as transverse sever, longitudinal split, etc. The proliferating neurons divided in an asymmetric way morphologically and chemically. The differentiated neuron appeared an obvious movement of cell migration and neurite extension.

结果：培养分化的神经细胞呈现横裂、纵裂等不同方式的无丝分裂像；分裂中的神经细胞可呈现形态及化学不对称性；分化的神经细胞有明显的细胞迁徙运动和突起延伸。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

4H was the best time of bovine oocyte activationin the 6-DAMP, and the cleavage rates and blastula rates could reach 85.1% and 26.8%. 4. IVF-100 was used in vitro fertilization, because IVF-100 was not needed to prepare usually,store conveniently, and gain a higher cleavage rates. After the oocytes have matured for 20h, theywere digested by permease and treated with suctionpipe.

由于IVF-100无需经常配制、保存方便且可以获得较高的卵裂率，所以本实验采用IVF-100为受精液，卵母细胞成熟20小时后采用透酶+口吸管消化的方法处理，获得了较好的受精效果，卵裂率可达(76.8％)。

To explore significance and effect of apoptosis in forming mechanism of cleft palate induced by dexamethasone.

探讨由地塞米松（dexamethasone，DEX诱导的腭裂胚胎模型的腭裂形成中细胞凋亡的意义与作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。