裂细胞

Results of HE dye after cerebral alchemic reperfusion showed: Cortex was infiltrated by neutrophil; Cells of corpus striatum and cortex of parietal lobe were stained light; the number off cells obviously reduced; the feature of cells necrosis, such as cells swelling, nuclear fragmentation and lysis et al, would be observed.

HE染色结果显示：脑缺血再灌注后，皮质区中性白细胞浸润，纹状体外侧和顶叶皮质细胞染色淡，细胞数明显 NF－。B在脑缺血再灌注后脑组织局部炎症反应中的作用减少，出现细胞肿胀，胞核碎裂和溶解等细胞坏死特征。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

It is well known that retinal pigment epithelial cells are predominant proliferative cells in PVR which is an excessively wound healing response occurring after RPE cell wounding. The activated RPE cells migrate from their normal, sessile state to vitreous and onto both surfaces of the retina, in which they begin to dedifferentiate, migrate, proliferate, change phenotype and secrete ECM.

既往研究证明视网膜色素上皮(retinal pigmentepithelial，RPE)细胞是参与PVR的主要细胞，PVR是视网膜脱离后RPE细胞损伤的一种自我修复过程，是一种过度的眼内创伤修复反应；RPE细胞在正常位置上处于静止状态，而在某些病理条件下，如视网膜裂孔形成和视网膜脱离后，RPE细胞脱离原位，开始去分化、移行、增生、发生表型转化并分泌胶原等ECM，最终在视网膜前后表面和玻璃体内形成具有收缩能力的增生膜，造成牵拉性视网膜脱离。

Methods: The model of evaluating T cell proliferation stimulated with polyclonal activators, ConA was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. The effect of a specific inhibitor for p38 MAPK, SB253580, on T cell proliferation on different doses and in different time was estimated by flow cytometry. At the same time, the percentage of proliferating T cells and proliferation index were measured by CellQuest and SPSS10.0 for Windows softwares.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立了在多克隆刺激剂刀豆蛋白A刺激下评价小鼠T细胞增殖的模型，通过流式细胞术分析p38丝裂原活化蛋白激酶的特异性抑制剂SB203580在不同剂量、不同时间对T细胞增殖的作用，并应用CellQuest和SPSS10.0 for Windows软件分析增殖细胞各代所占比例和增殖指数。

After treated with the samples, the MGC-803 cells are dyed with the fluorescence dye Hoechst 33258. Observed by the fluorescence microscope, the dense dyeing granulometric and nubby blue fluorescence can be seen in the nucleus of MGC-803 treated with AK-9 and AK-11, the nucleus of some cells smash to small fragments.

采用荧光染料Hoechst 33258对经初筛活性样品处理的MGC-803细胞染色，荧光显微镜观察，发现经AK-9及AK-11发酵液处理的细胞，其细胞核内可见浓染致密的颗粒或块状荧光，细胞核发生固缩、碎裂，而对照细胞的细胞核呈均匀弥散荧光，表明这2株菌株可能产生具有诱导细胞凋亡的活性物质。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖，使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长，光镜下见细胞突起变短，细胞由梭形变成椭圆形，核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

The proliferating cells were mainly distributed in the peri-chondrium and the peripheral zone of tracheal cartilage. The senescent chondrocytes were distributed in the central zone whereapoptotic morphological characteristics obviously appeared, such as pyknosis, karyorrhexis.

结果：软骨细胞呈现多种方式的无丝分裂像；气管软骨膜与软骨周围带多见细胞增生；气管软骨中央带多见衰老的软骨细胞，并见明显核固缩、核碎裂等细胞凋亡形态学特征。

Hepatocyte growth factor/scatter factor is a multifunctional cytokine with mitogenic, motogenic, and morphogenic activities for renal tubular cells.

肝细胞生长因子是一个对肾小管上皮细胞具有强烈丝裂原活性以及细胞运动与形态调节的多效性细胞因子。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下，与对照组相比，经姬姆萨染色观察，西咪替丁、尼扎替丁处理的SW480细胞，先发生皱缩，体积变小，异形性减少，细胞变圆数目增多，细胞核变小，染色变淡，核仁数量减少，部分可见染色质边集现象；细胞间连接疏松，随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后，SW480细胞全部变圆，细胞核碎裂，固缩微核形成，部分不再贴壁。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。