裂细胞

In the first part of the study, it was demonstrated that there was only 0.1% of PCV2 antigen-containing rate in PBL isolated from PCV2-free pigs and treated simultaneously with mitogen and PCV2. The results of both MTT assay and blastogenesis indicated that PCV2 could reduce the responsiveness of PBL to mitogen stimulation, but the results of survival and apoptotic rates indicated that PCV2 had no effects on the viability of PBL.

首先，在第一阶段实验中针对健康无PCV2带原的猪只进行测试，当同时对其PBL以致裂原刺激与PCV2攻毒处理后，其PBL中仅0.1％的细胞呈现PCV2抗原阳性；藉由MTT与blastogenesis分析，均显示PCV2可以降低PBL对於致裂原刺激的反应，然而PCV2对於PBL的存活率或细胞凋亡率则无显著影响。

We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料（Hoechst-33342）对核移植体进行染色的方法，对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察，确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

Early embryonic cell has the developmental totipotency. Every embryonic blastomere cell can develop to an intact individual separately in a proper condition.

早期胚胎卵裂球细胞具有发育全能性，每个胚胎卵裂球细胞在适宜的条件下都能单独发育为一个完整的个体。

Microbiopsy of pre-embryos with 4 or 8-cells did not alter their viability and further development. The technique of nested PCR on a single blastomere for the detection of cystic fibrosis ΔF508 mutation is reliable.

在小鼠胚胎4 细胞或8细胞阶段实施单个卵裂球显微活检，不影响胚胎的进一步分化以及小鼠出生后正常生长发育；利用显微活检得到的单个卵裂球进行突变基因诊断是可行的。

Compared with the corresponding BEAS-2B cells, the shapes of cells and nuclears, the shapes and quantity of cell organs were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The 10th, 15th passage BEAS-2BNNK cells, which had swelled cell and cell organs and abnormal nuclear and enlarged the nucleoli gradually and increased the quantity of the cell organs and activated their function, showed the characteristic of transformation cells. The 20th, 25th passage BEAS- 2BNNKcells, which had obvious aberration of the cell and nucleoli and had cataclasm of nucleoli and decreased cell organs, showed the characteristic of tumor cells.

与BEAS-2B相比较BEAS-2BNNK第1、2、5代细胞及胞核形态、细胞器形态及数量无明显差别；第10、15代细胞出现肿胀，细胞核逐渐变形，出现核畸形，核仁明显增大，边集，细胞器肿胀，数量明显增多，功能活跃具明显的转化细胞特征；第20、25代细胞及胞核明显畸变，出现明显的核碎裂及多个核仁，细胞器明显减少，具明显的肿瘤细胞特征。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示：VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性；经4mmol/LVPA处理MUTZ-1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞装内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

Trophozoite,macrogamout,microgamout, typeⅠmeront,typeⅡmeront and sporozoite in the development of C.andersoni were observed by TEM.The proliferation of C.andersoni was significantly inhibited by 1.25μg/ml NTZ and 0.31μg/ml GAs.Treated with 20μg/ml NTZ and 5μg/ml GAs respectively,the numbers of C.andersoni were only 0.29%and 0.66%compared with the untreated.

透射电镜在HCT-8细胞中观察到安氏隐孢子虫的滋养体、雌配子体、雄配子体、Ⅰ型裂殖体、Ⅱ型裂殖体和子孢子阶段。1.25μg/ml NTZ和0.31μg/ml GAs即可显著抑制HCT-8细胞中安氏隐孢子虫的增殖。20μg/ml NTZ和5μg/ml GAs作用后，虫体数量仅分别为未加药的0.29%和0.66%。

Analysis of mitochondrial DNA composition of the panda-rabit cloned embryos found that mitochondria from both panda somatic cells and rabbit ooplasm co-existed in early blastocyst, but mitochondria from rabbit ooplasm decreased and those from panda donor cells dominated in early fetuses after implantation.

结果显示，应用卵裂球电融合方法可以制作一系列的多倍体胚胎；小鼠囊胚的形成与胚胎中的细胞数目无直接关系，卵裂球的电融合和染色体数目的增加不会改变胚胎的发育进程；而胚胎细胞中的核／质比可能是控制胚胎囊胚化的一个重要因素。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Sowe selected the colchicine optimum parameters were 0.6μg/ml and 6 hour culturedduration.The second, there were no significantly differences between the four groupsin 2 hour groups; and there were significantly differences between 0.2μg/ml groups and other three groups in 4 hour groups; and there were no significantlydifferences between the four groups in 6 hour groups; and there were significantlydifferences between 0.4μg/ml groups and other three groups in 8 hour groups onthe metaphases (P＜0.05), in the experiment of the effect on the mouse 8-cellembryo stage single blastomere of colchicine with different concentrations andduration by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.6μg／ml和6小时。2、在确定秋水仙素不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养2小时组中四个浓度组差异均不显著；阻断培养4小时组中0.2μg／ml浓度组与其他三组差异显著；阻断培养6小时组中四个浓度组差异均不显著；阻断培养8小时组中0.4μg／ml浓度组与其他三组差异显著(P＜0.05)。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。