裂细胞

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上结果显示，6－DMAP能增加胚胎干细胞异种重构卵的卵裂率，对重构卵囊胚的发育率影响不大；高培养代数和低培养代数的胚胎干细胞用于异种核移植不影响异种重构卵的卵裂率和囊胚发育率；用胚胎干细胞作为供核细胞；比体细胞作为供核细胞所构建的异种重构胚更容易进行重编程，并且胚胎于细胞指导异种克隆胚胎正常发育的能力强于体细胞。

Medication during early pregnancy can cause fetal spina bifida, cleft palate, clubfoot, small limbs, Phocomelia, umbilical prominent cheek transverse fissure, cerebral meningocele, cerebral spinal cord dysfunction, osteogenesis imperfecta and fetal death. 2, prednisone: big neat administration during early pregnancy can cause stillbirth, streaming, no brains infants, small ectopic kidney, one-eyed, cleft palate, bone deformities, such as. 3, 6-8 weeks pregnant villus chromosome examination; pregnant 16-20 weeks to do the amniotic fluid puncture in amniotic fluid cell chromosome examination; At the same time, dynamic observation of maternal alpha-fetoprotein content; attention fetal B-track general shape and structural abnormalities offal.

妊娠早期用药可致胎儿脊柱裂，腭裂，畸形足，小肢，短肢畸形，脐突出，颊横裂，脑脊膜膨出，脑脊髓功能障碍，成骨发育不全及胎儿死亡。2、强的松：妊娠早期大齐整给药可引起死产、流、无脑儿、小异位肾、独眼、腭裂、骨畸形等。3、孕6-8周取绒毛组织进行染色体检查；孕16-20周行羊水穿刺做羊水细胞的染色体检查；同时，动态观察母血中的甲胎蛋白含量；注意B超跟踪胎儿大体形态和内脏结构是否异常。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

The formation of silique dehsicence susceptibility was investigated in terms of water content, synthetic rate of ethylene, enzyme activity of exo polygalacturonase and end cellulase , cell alteration in dehiscence zone in silique and silique dehiscence susceptibility during silique development.

从油菜角果发育过程中角果含水量、乙烯合成速率、外切 PG 酶和内切纤维素酶活性，以及角果裂区切片观察等方面研究了角果开裂敏感性形成的原因⒚花后50d 的角果果柄端壳状果瓣和线状果瓣间的裂区薄壁细胞膨大，在高倍光学显微镜下可见胞间空隙开始形成⒚此前6d 内乙烯合成速率迅速上升并出现第二次合成高峰，与之同步发生外切 PG酶和内切纤维素酶活性急剧上升，而乙烯合成第二高峰出现于角果含水量低于80％以下⒚由此看来，角果失水引起乙烯合成第二高峰出现，从而导致水解酶活性上升，最终导致果瓣裂区薄壁细胞膨大，分离和胞间空隙形成及角果开裂敏感性产生

The result of frozen MⅡoocytes using three methods indcated that the self-restraintspear method was better than OPS, and the effection of frozen altogether with self-restraintspear method and centrifugal was the best; the frozen effection was not ideal using fluidspear for 8- cell ovocyte.

用三种冷冻方法(OPS法；自制移液枪头法；移液枪头法+离心法)对MⅡ卵母细胞冷冻的结果表明，自制移液枪头法优于OPS法，其中以移液枪头法+离心组的冷冻效果最好；用移液枪头法对体外发育到8-细胞期卵母细胞的冷冻效果不理想。MⅡ期卵母细胞用OPS法冷冻后卵母细胞IVF和电激活的卵裂率都显著高于移液枪头法(P＜0.01)，电激活后2-细胞卵裂率比IVF高。

We carried out the experiment that edelfosine acted on the nucleus division and detected the DNA content of S. pombe, and analyzed the effect of edelfosine on nucleus division of S.

(1)、应用粟酒裂殖酵母细胞和Jurkat细胞的生长抑制试验，确定依地福新抑制粟酒裂殖酵母细胞生长的作用剂量。

The sixth, there were no significantly differences between colchicine groupsand vinblastine groups on the metaphases, in the experiment of prepare mouse4-cell embryo stage single blastomere chromosome specimen by using optimumparameters; and there were significantly differences between colchicine groupsand vinblastine groups on the metaphases (P＜0.05), in the experiment of preparemouse 8-cell embryo stage single blastomere chromosome specimen by usingoptimum parameters.The end, the preparation quality of mouse 4-and 8-cell embryo stage singleblastomere specimen were 2.5, 2.7, 2.4, 1.9, respectively.

低渗液选用0.45％氯化钠时，低渗效果优于选用0.5％柠檬酸钠和0.35％氯化钾的。6、在利用最佳参数制备小鼠4-细胞期胚胎单卵裂球染色体标本时，经统计分析，两种阻断剂的作用效果差异不显著；而在制备小鼠8-细胞期胚胎单卵裂球染色体时阻断剂选用长春花碱的效果优于秋水仙素的(P＜0.05)。7、秋水仙素组和长春花碱组小鼠4、8-细胞胚胎单卵裂球染色体标本制备质量分别为：2.5、2.7、2.4、1.9。

Pombes growth inhibition experiment. The difference has statistic significance (P＜0.01) between these cell treated with 5.0μM, 10.0μM and 20.0μM edelfosine for 6 h and those cell treated with 0μM edelfosine for 6 h.

利用粟酒裂殖酵母细胞胞核分裂试验和粟酒裂殖酵母DNA含量检测试验，观察依地福新对粟酒裂殖酵母细胞胞核分裂的影响。

Fission A type of asexual reproduction in which a parent cell divides into two daughter cells genetically identical to the parent. Binary fission occurs in many unicellular organisms, such as protoctists, bacteria, and fission yeasts.

裂殖：无性生殖的一种类型，指一个母细胞分裂成两个与母细胞相同的子细胞，二分裂发生在许多单细胞有机体中，如原生生物、细菌和裂殖酵母。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。