裂殖子

Merozoite Surface Protein 1 of Plasmodium falciparum...

恶性疟原虫主要裂殖子表面蛋白1（PfMSP1）是当今领先的疟疾疫苗候选抗原，其主要实验依据是来自夜猴攻击试验。

Merozoite Surface Protein 1 of Plasmodium falciparum(PfMSP1) has been considered a leading candidate for malaria vaccine.

恶性疟原虫主要裂殖子表面蛋白1（PfMSP1）是当今领先的疟疾疫苗候选抗原，其主要实验依据是来自夜猴攻击试验。

Merozoite Surface Protein 1 of Plasmodium falciparum (PfMSP1) has been considered a leadi.

恶性疟原虫主要裂殖子表面蛋白1（PfMSP1）是当今领先的疟疾疫苗候选抗原，其主要实验依据是来自夜猴攻击试验。

P.vivax ; merozoite surface protein 1（MSP1）; genotype ; morphology

间日疟原虫；裂殖子表面蛋白1；基因型；形态学

Two fragment of Etmic-2 was cloned and sequenced by PCR using genomic DNA as template from sporulated oocysts and the second generation merozoite of Eimeria tenella.

本文利用PCR技术，分别从柔嫩艾美耳球虫的第二代裂殖子和孢子化卵囊基因组DNA中扩增出微线蛋白EtMIC-2基因序列，并进行了克隆和核苷酸序列测定。

Objective To determine the invasive ability of recombinant attenuated Salmonella typhimurium X4064（pQEM1）strain containing gene fragment of Plasmodium falciparium merozoite surface protein 1 （MSP1）,the stability of its plasmid, and its re-expression.

目的 检测含恶性疟原虫裂殖子表面蛋白1基因片断的重组减毒鼠伤寒杆菌X4064(pQEM1)的入侵宿主能力，所含质粒的稳定性和再表达能力。

Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1(MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition.

摘 要：目的检测以恶性疟原虫裂殖子表面蛋白1的17区片段基因为基础的复合核酸疫苗(分泌性的VR1012/TPA/HG-MSP1-17和非分泌性的VR1012/HG-MSP1-17)诱导小鼠的体液免疫反应和免疫血清对疟原虫生长的抑制能力。

AIM: To analyse the humoral immune response in mice to nucleic acid vaccines ( VR1012/HG MSP1 17 for intracellular expression or VR1012/TPA/HG MSP1 17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL 1 and TT.

目的： 检测以恶性疟原虫裂殖子表面蛋白1 的17 区片段基因为基础的复合核酸疫苗VR1012/TPA/HG-MSP1-17和VR1012/HG-MSP1-17诱导小鼠的体液免疫反应。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！