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Phage display of recombinant human lymphotoxin mutation librariesand receptor affinity screeningContraction of conformable large molecule mutation library is pivotal for molecular evolution study in vitro. In order to extend the range of the screening region of amino acid in LT gene, several receptor binding sites of LT were mutated randomly by overlap PCR amplification with the random nucleotide primers, the R46, S106, L130 combined site-directed random mutation library and R46~A52, S106~F110, R46~A52 + S106~F110 region random mutation libraries of rhLT were constructed successfully.

二。重组人淋巴毒素噬菌体库的构建及受体亲和筛选构建适合的大容量生物大分子变异体文库是进行体外分子进化研究的关键,为了扩大LT基因序列中被筛选的氨基酸范围,我们采用含随机核苷酸序列的引物,通过Overlap PCR的方法对LT的受体结合区域进行多点随机突变,分别构建了rhLT R46+S106+L130三点随机突变组合文库和R46~A52、S106~F110、R46~A52+S106~F110区域随机突变体库。

Our country current industrial economic benefits comprehensive evaluation index system by total assets contribution rate, such seven indexes as capital value preserving and appreciation rate , asset-liability ratio , turnover rate of the current assets , expenses profit rate of the cost , sales rate of the products , overall labour productivity ,etc.

对我国工业经济效益的考核,迄今为止采用的仍是1998年进行试算,1999年按月对外发布的工业经济效益考核指标体系,经过6年多的运行,已经趋于成熟,一些问题却也随之暴露出来。同时,随着工业经济形态向知识经济形态的转变,可持续发展问题日益被人民所重视,客观上对工业经济效益的考核提出了新的要求,故对我国现行工业经济效益考核的指标体系做如下探讨。

To this end the original 'Blue Fox' radar is replaced by Ferranti 'Blue Vixen' coherent pulse-Doppler track-while-scan radar to improve acquisition and look-down capabilities, and other improvements are a MIL 1553B digital databus (making it possible to carry Sea Eagle anti-ship and ALARM anti-radar missiles) and the installation of the Marconi Sky Guardian 200 RWR, the JTIDS for secure voice and data links, two additional underwing hardpoints, wing tip stations for two AIM-9 Sidewinder short-range AAMs, wing improvements, a revised cockpit with HOTAS controls, larger drop tanks, provision for 25 mm Aden 25 cannon in place of the elderly 30 mm weapons, and possibly a Plessey missile-approach warning system.

为实现这一目的,原有的"蓝狐"雷达被新型的"蓝雌狐"连续波脉冲多普勒雷达替换,该雷达具有搜索的同时锁定目标的能力,可下视下射。其他改进包括加装军用1553B数据链,这使得MK2可以使用"海鹰"反舰导弹和ALARM反雷达导弹。另外加装了马可尼"天空哨兵200"通信系统,JTIDS保密语音及数据链路。增加了两个翼下外挂点,翼尖挂架可携带两枚AIM-9"响尾蛇"近距空空导弹。细节的改进还包括:机翼设计有所改进;改进的座舱设计;更大的副油箱;采用阿登25mm机关炮代替了以往的30mm机炮;可能加装了导弹告警系统。

In order to design a scheme to quantify the observation and to get the fusion estimation from the received bits,the estimation fusion problem was changed into that of decision fusion.The interval was divided into subintervals,each subinterval was assigned a hypothesis,suitable cost coefficients of each hypothesis was selected to form the Bayes cost functional,and a mapping from estimation fusion to decision fusion was constructed.

采用将区间划分,每一区间形成一个假设,规定假设之间合适的损失系数,将估计融合问题化为决策融合问题,运用成熟的决策融合的方法,对于现在研究还不成熟的估计融合传感器量化问题设计了一个解决方案,将决策融合的决策结果作为最终的估计,从而在观测被量化的条件下达到较优的估计效果。

Second, aminolysis of polystyrene and poly prepared by reversible addition-fragmentation chain transfer polymerization was investigated. The product of the former contains predominantly double molecular weight species by the formation of disulfide bond, whereas the latter formed coupled species which consequently cleaved to unimolecular weight species.

由RAFT聚合的机理可知,聚合最终得到的聚合物的结构包含双硫酯基团,它是聚合物链保持活性特征的重要体现,同时它为聚合物的端基官能化提供了一个重要的途径,其中氨解反应是一个被人们广泛采用的方法,它在很温和的条件下将端基去掉,同时还能将双硫酯端基变成硫醇端基。

This invention relates to one large size space coordinate measurement technique and device based on automatic visual guidance, which aims to provide one coordinate measurement and device and adopts the following scheme: altometer fulfills the points to be tested for automatic identification and measurement through variable cameral guidance to realize the large size space coordinate identification and fine measurement by each crossing constraints.

本发明的目的是提供一种基于自动视觉引导的大尺寸空间坐标测量方法及检测装置,该装置具有测量速度快、非接触、能在恶劣的环境中使用、较高的测量重复性特点,本发明采用的技术方案是,经纬仪通过可变焦摄像机的引导完成对被测点的自动识别及测量,由各经纬仪间的交汇约束实现大尺寸内空间坐标的自动识别及精密测量。

Differential modulation is also a widely used method to the case of no knowledge of the channels in the receiver. Here we propose noncoherent sequence detection for differential space-frequency modulation, which can be used to alleviate the error floor that occurs in a multipath channel with long time delay expansion.

在接收端不知道信道状态信息的情况下,差分调制也是一种被广泛采用的技术,论文提出了应用于差分空频调制的非相干序列检测方法,该方法通过积累多个符号上的信道信息来完成发送符号的检测,可以消除由于长的信道延迟扩展带来的误码平层。

Under the definitions of the probability and the expected values of unhit attacking missile quantity,the expected values of anti-missile quantity and attacking missile quantity hit by a single anti-missile,the weapon operation efficiency was presented at various engagement pattern...

在定义未被命中的来袭导弹概率和数学期望、反导导弹数量的数学期望、单发反导导弹命中来袭导弹数学期望概念的基础上,研究不同作战方式下的武器作战效能。结果表明:提高导弹武器的效能,最为可行的方式是采用2发为单位的齐射。

This may be the crucial test for Mr Dell who, after all, gave his name not just to the firm but to a whole business model (just-in-time, made-to-order manufacturing using an outsourced supply chain), key elements of which have since been adopted by his competitors.

这个问题可能是对戴尔先生的一个重要的考验,毕竟,他不仅仅是将他的名字给了这个公司,而且是给予了整个商业模式(使用外包供应连锁商的按时生产、按订单制造的制造业),这个模式的关键成分已被他的竞争者们所采用。

In the method, the polyclonal antibodies are obtained by utilizing syntactic quinoxalone antibiotics multi-cluster antigen immunization; the coupling compound of the half antigen of the quinoxalone antibiotics and an egg-white protein OVA is taken as an envelope antigen; the quinoxalone antibiotics is taken as a standard; the indirect competitive enzyme-linked immune detection method for the quinoxalone antibiotics in animal food is established; a rapid and high efficient detection means is provided for the residues of the quinoxalone antibiotics in the animal food; because the polyclonal antibodies are adopted, the cost is low, and the stability and the repeatability are good; the detection limit (IC is smaller than 90) is 0.0126 ng/ml, the median inhibitory dose (IC is smaller than 50) is 0.52 ng/ml, and the detection range (IC is smaller than 20 to 80) is 0.04467 to 14 ng/ml.

本发明利用合成的喹诺酮类抗生素多簇抗原免疫得到多克隆抗体,以喹诺酮类抗生素半抗原与卵清蛋白OVA的偶联物作为包被抗原,以喹诺酮类抗生素为标准品,建立动物性食品中的喹诺酮类抗生素的间接竞争酶联免疫检测方法;为喹诺酮类抗生素在动物性食品中的残留检测提供了快速高效的检测手段,由于采用的是多克隆抗体,费用较低并且稳定性和重复性较好,检测限(IC 90 )为0.0126ng/ml、半数抑制量(IC 50 )为0.54ng/ml和检测范围(IC 20 ~IC 80 )为0.04467~14ng/ml。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。