被膜下的

Several masses of condensed chromatin without nuclear membrane were observed in some treated K562 cells,while chromatin of some other cells tended to marginate in crescents around the nuclear envelope with compacting cytoplasm,normal or slightly expanded endoplasmic reticulum,which presented typical morphology of apoptosis.

电镜下，加药处理组中部分细胞染色质凝集成块，没有核膜包被，部分细胞染色质靠核膜边集呈新月形，胞浆浓缩，内质网结构正常或有扩张，呈现典型的凋亡细胞形态。

Fatsia japonica、 Nandina domestica、 Laurus nobilisl、 Hhypericum Chinense were cultivated under four different light regimes(fully open site,58%,24% and 6% daylight).Their leaf area, leaf mass per unit area, the gas exchange parameters , chlorophyll content, the RPP were measured.

测定了4种光强(100％、58％、24％、6％自然光强)下，地被植物八角金盘、南天竹、月桂、金丝桃的叶面积、叶片气体交换参数、比叶重、叶绿素含量和高温下细胞质膜透性。

The monolayers of LPSQ can be successfully transferred onto hydrophobic surface of silicon wafer or quartz plate to develop a very stable and uniform Y-type Langmuir-Blodgett film when using toluene as the spreading solvent at proper surface pressure.

在适当的表面压下，以甲苯为铺展溶剂该单分子层可以被成功地转移至疏水的硅片或石英片上形成均一稳定的Y-型LB膜，其紫外吸收强度在几个月内均保持不变，说明该LB膜具有良好的稳定性，AFM图像表明其形成了均一致密的膜。

Hyphae, loaded with CTC under pH 6. 8 condition and then grown in pH 8. 0 medium for a little while to destroy the apical acidification, could gave very diffuse fluorescence images of CTC membrame bound Ca〓 and vermiform fluorescence spots due to mitochondrias under pH 6. 8 no longer were clearly distinquished, which implied that besides mitochondrias, other cellular organelles with Ca〓 also were stained with CTC. Thus mitochondrias are not the only one intracellular Ca〓 storage, endoplasmic reticulums and Golgi bodies in the same zone may be also the Ca〓 storages. But the extreme apical zone under 2μm of the growing hyphal tip was still almost devoid of stain under pH 8. 0.The CTC fluorescence was concentrated in the subapic zone beyond about 2μm from the tip and then gradually became lower behind about 40μm from the tip. These results did not support the hypothesis which suggested that the cell wall vesicles in the extreme apical zone were intracellular Ca〓 storages.

将菌丝在pH6.8条件下负载CTC，再置于pH8.0培养介质中短暂生长一会儿以消散菌丝顶端的酸化区域，则CTC膜结合Ca〓呈现弥散的荧光影像，在pH 6.8培养条件下显示的蠕虫状的线粒体荧光斑点不再能够清晰辨认，说明除了线粒体之外，还有其它含Ca〓细胞器，也被CTC染色，线粒体并不是细胞内唯一Ca〓库，还可能包括内质网、高尔基体等细胞器，但菌丝最顶端2μm以前的细胞壁泡囊区域不能被染色，最大荧光强度仍位于菌丝顶端2μm以后区域，约45μm以后荧光变弱，实验结果不支持细胞壁泡囊为菌丝胞内Ca〓库的假设。

Several studies indicate a worse outcome in patients who received fasciotomy compared with those who did not.7, 11 It is felt to be indicated only in a potentially viable extremity that is pulseless due to elevated intracompartmental pressure.18 Fasciotomy was felt to be useful in preventing cases of Volkman's ischemic contracture after the Tangshan earthquake.3 In Israel, fasciotomy was reserved as a treatment of last resort in cases refractory to the use of intravenous mannitol.7

几项研究表明，采用筋膜切开术的患者后果比未手术者更严重。这说明仅在可能的极端情况下筋膜间区压力升高才会导致无脉动现象。在唐山地震后，筋膜切开术对预防前臂缺血性肌挛缩有效。在以色列，筋膜切开术被做为难以采用静脉输注甘露醇病人最后的治疗方法。

Firstly, the acute cytotoxicity of profenofos on FG-9307 cells was studied, it was found that the cell growth rate was markedly reduced by profenofos at all the concentrations tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysosomes with engulfed organelles and numerous vacuoles in the cytoplasm.

丙溴磷对FG-9307的急性细胞毒性研究结果发现：1）在所有实验浓度下，细胞的生长速度均受到抑制。2）细胞的超微结构被丙溴磷所改变，最明显的特征则是线粒体损伤严重，嵴肿胀，部分溶解破裂；核膜肿胀，有溶解；细胞内的电子密度高的溶酶体增多；内质网的膜溶解，核糖体从粗面内质网上脱落下来，游离于胞质中。

The assembly of journal and plain bearing liner space is too little, force of organic still oil pressure is small it is the crucial index that affects lubricant result, make lubricate because of lacking engine oil between crankshaft and plain bearing liner and bite dead invalidation, include to be pulled badly, lack lube, layer of alloy of dry attrition generation is fused, grind sliding surface to did not maintain the oily film of certain ply, and all sorts of local overload exhaustion are destroyed, cavitation erosion and V form are destroyed etc, through technology of him director diesel locomotive old experience reachs the analysis of pair of its breakdown reasons, put forward precautionary measures especially. As a result of,the burns caustic mechanism plain bearing liner to burn caustic mechanism of 1 plain bearing liner is the lube film burst between journal and plain bearing liner, cause plain bearing liner and crankshaft generation clash, below high temperature, high pressure and high rotate speed, the wear-resisting alloy layer of plain bearing liner wears away prematurely or fuse, cause plain bearing liner and crankshaft to stick thereby bite, can make the steel back of plain bearing liner and Kong Zhong produces whirligig, inlet port is jammed, carry on the back by steel again with the friction of aperture causes high temperature to make plain bearing liner burned-out further, can cause bolt to break off, happen swing a malign accident that pounds cylinder body of bad internal-combustion engine.

轴颈和轴瓦的装配间隙太小，还有机油压力低是影响润滑效果的关键指标，使得曲轴和轴瓦之间因缺少机油润滑而咬死失效，包括严重拉伤，缺少润滑油，干摩擦产生合金层熔化，磨滑面没有保持一定厚度的油膜，以及各种局部过载疲惫破坏，气蚀和V形破坏等，通过本人主管内燃机车技术多年经验及对其故障原因的分析，特提出预防办法。1轴瓦的烧损机理轴瓦烧损的机理是由于轴颈和轴瓦之间的润滑油膜破裂，导致轴瓦和曲轴产生摩擦，在高温、高压和高转速下，轴瓦的耐磨合金层过早地磨损或熔化，从而引起轴瓦和曲轴粘咬，会使轴瓦的钢背和座孔中产生旋转运动，进油孔被堵塞，再由钢背和座孔的磨擦进一步引起高温使轴瓦烧坏，并会造成螺栓折断，发生甩出捣坏内燃机缸体的恶性事故。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

The result using inhibitors of Ca〓 channels supported above observation. When Ca〓 channels in plasma membrane were blocked by LaCl〓, verapamil or nifedipine, and the Ca〓 channels in endomembranes opened naturally, extracellular CaM couldn't induce 〓 increase. When Ca〓 channels in endomembranes were blocked by ruthenium red and the Ca〓 channels in plasma membrane opened naturally, extracellular CaM induced Ca〓 increase in guard cells, but the extent of Ca〓 increase was lower than that of control.

使用质膜Ca〓通道抑制剂LaCl〓、异博定、尼群地平及细胞内Ca〓通道抑制剂钌红的结果支持这种推测：在质膜Ca〓通道被阻断、细胞内Ca〓通道仍正常开放的情况下，保卫细胞〓不能被CaM诱导增加；细胞内Ca〓通道被抑制、质膜Ca〓通道正常开放的条件下，细胞外CaM诱导保卫细胞内〓升高的幅度不能达到不使用Ca〓通道抑制剂的水平。

Result vitamin A and 4HPR fall to Hela cell collect form have apparent inhibition, can restrain Hela cell to be opposite the action causing tumor of naked rat (the curb tumour action of P.01); vitamin A issues visible cell solid by force to shrink at 4HPR(P.01); report lens, nuclear film is screwy, nuclear chromosome gathers agglomerate stand by the wither such as nuclear film to die DNA of cell of cellular feature; by degradation, a collection of illustrative plates of typical " of shape of " a flight of stairs appears in electrophoresis of agaragar candy gel.

结果维生素A和4HPR对Hela细胞集落形成有明显抑制功能，并可抑制Hela细胞对裸鼠的致瘤功能(P.01)；维生素A的抑瘤功能强于4HPR(P.01)；电镜下可见细胞固缩，核膜扭曲，核染色体聚集成块并靠近核膜等凋亡细胞特征；细胞DNA被降解，在琼脂糖凝胶电泳中呈现典型的&阶梯状&图谱。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。