被抑制的

Additionally XBP1 mRNA which functioned as centrally regulating molecule during ER stress was spliced partially,while it was completely spliced in positive control cells and unspliced in H7721.These results gave the proof that blocking expression of GnT-V caused H7721"s ER stress and response was more weak than that caused by DTT. It may be the chronic process.Additionally,previous studies reported that the ds-RNA may activate the kinase PKR which phosphorylated eIF2 α and inhibited synthesis of proteins.In this paper the antisense cDNA of GnT-V was integrated with GnT-V-AS/H7721"s gene and functioned through the binding of antisense RNA and mRNA of GnT-V.Additionally,the integration of GnT-V cDNA in genome also may be a non-specific factor.

对这三种ER stress关键分子的检测发现：和阴性细胞相比较，实验细胞中BIP的表达无论是蛋白水平还是转录水平都有明显的上调，但上调程度都要低于DTT处理的ER stress阳性细胞；XBP-1 mRNA在实验细胞中部分被剪接，在阳性细胞中XBP-1 mRNA完全被剪接，而在阴性细胞中其以非剪接形态存在；此外和DTT处理的ER stress阳性细胞相似，实验细胞中的PERK发生磷酸化，表明ER stress过程中通过磷酸化eIF2α抑制蛋白合成机制的活化，这和芯片所检测到的GnT-V-AS／H7721细胞蛋白合成系统水平下调相一致。

A thermoplastic layer material has at least one odor-reducing surface which is wettable to aqueous liquids and capable of controlling a wide variety of malodors.

一种热塑性层状材料，具有至少一个能减弱臭味的表面，该表面能被含水液体润湿，并能抑制多种臭味。

We speculated that ZAD domain may be related to some special physiologic or metabolic processes in insects. The distribution of C2H2 ZFP genes in the genome was investigated.

除了具有锌指结构域外，部分锌指蛋白还含有其它类型的结构域，被称为锌指偶联结构域，它们能辅助锌指蛋白激活或抑制目标基因表达。

Inactivation of the enzyme folioed an elhance of GndHClconcentlation. Half of the ovelall reaction activity was lost within l.5 Ininuthe in 0.54Inol/LGndHO, and 5% achvity remained at the end of inachvation.

在低浓度盐酸胍溶液中该酶的全反应活性和其中两个还原部位单独活性被同步可逆抑制，随着胍浓度增高，出现不可逆失活且程度和速度均迅速提高，在0.54mol／L胍中该酶全反应活性在1.5分钟内已有一半不可逆丧失，终态只余约5％活性。

It has been hypothesized to be a risk factor for anxiety disorders.

这种行为抑制表现被认为是焦虑障碍的一个危险因素。

At the present time,some genes ,such as luteinizing hormone gene and inhibin gene,have been found to be associated with the susceptibility to POF.

现在，黄体生成素及抑制素基因等一些基因已被发现与POF的易感性有关。

C released ratio from sowing and transplanting rice ecosystems were 58\^0%～75\^0%,most of which was in form of volatilizing 14 C, a part of which existed in soil, and only a small part was absorbed by rice seedlings and inhibited the growth.

在 1、2、3、4、6μg/ g土14C BR0 中，于直播水稻和移栽苗(4μg/ g土14C BR0 )分别经 3 9d和 2 9d后，14C释出率分别达 75 0 %、68 7%、62 6%、5 8 0 %及 70 4 %。其中绝大部分以挥发性态14C逸走，其次存留在土壤中，少量被秧苗吸收，并能抑制秧苗的发育生长。

RESULTS: The absorption of emodin was reduced by aloe-emodin and physcion conspicuously.

结果：大黄素的吸收被芦荟大黄素、大黄素甲醚显著地抑制。

Once the connection between chloramphenicol and the procaryotic ribosome was discerned, it made sense that under certain conditions, chloramphenicol might inhibit eucaryotic cells.

抗生素和原核性核糖体的关系被发现后，马上就使人意识到在某种情况下，氯霉素也许能抑制真核细胞。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。