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Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P<0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P <0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

Fiber formation and lignification were also decreased.

同时纎维化和木质也被抑制

Sessile marine coelenterates including solitary and colonial polyps; the medusoid phase is entirely suppressed.

座生的海洋腔肠动物,包括居独和群居的水螅;水母阶段完全被抑制

For example, for terminal A's connection, signal output to terminals B and C is suppressed.

例如,对于终端A的连接,输出至终端B和C的信号将被抑制

Playing time before going to bed too long, over-excited; or frightened during the day, feelings of anxiety, so that the spirit can not be inhibited down.

疲劳过度。睡前玩的时间太长,兴奋过度;或白天受了惊吓,心情焦虑,使精神不能很好地被抑制下来。

She knew she loved Chris that much because of her pent-up love and terror for Jerry.

她知道她这样地爱克里斯是来自于她被抑制住的对杰里的爱和对杰里的担心)。

Implementing good solid phase control, giving full play to peridium inhibitability of polymerized substance driven well fluid, keeping good rheologicability, sand carrying ability are considered to satisfy the requirement under well, reduce drilling resisting exte...

认为:在目前尚无更适合该地区新型钻井液体系的情况下,只要很好的实现固相控制,充分发挥聚合物钻井液的包被抑制性,保证钻井液具有良好的流变性、携砂性和滤失造壁性,也能较好地满足井下要求,降低阻卡程度,减少井内事故。

The optical properties, fluorescence quantum yields and electrochemical properties of these polymers were investigated. It was found that the optical properties of the polymers did not show obvious dependence on the alkyl length on phenylene.

苯环上烷基侧链长度的改变对取代共聚物的光谱、电化学和发光效率等影响很小;而随着苯环上烷氧基侧链长度的增加,聚合物的光谱稳定性逐渐增强,荧光发射光谱中的0-1转变逐渐被抑制,荧光发射半峰宽减小。

By projecting signals with different frequencies into the subspace I, the signal with the supply frequency has the maximum value. Consequently, in the spectrum of the reciprocal of the projecting value, the supply frequency component is cut off and the fault feature component emerges in the spectrum.

把不同频率的信号投影到子空间I,基频信号在该空间的投影将远大于其他的频率分量,因此在投影的倒数谱中,基频分量被抑制,凸显出了故障频率分量。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?