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Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

The vertical distribution of soil water also changed obviously. Soil layer 0-20cm was greatly impacted by the outside conditions and had greater coefficient of variance than the sub-layer. The greater water was consumed the more significant water shortage was showed in surface soil under sodding culture.

此外,稻草覆盖和自然覆被分别抑制了69.10%和36.53%的土壤蒸发,其蒸发抑制作用在午间高温时段表现尤为明显,使得更多的水分以植物蒸腾(稻草覆盖15.71%、自然覆被7.30%)和土壤蓄留的方式支出,并且分别降低了20.55mm和14.33mm的水分支出。

The degradation of pollen tube RNA in vitro by stylar S-RNase at different temperatures was performed to detect the extent of degradation using the agarose gel electrophoresis. The germination and tube growth of self-pollen and the RNA of self-pollen tube were inhibited and degraded significantly greater than cross-pollen respectively at 10℃ and 25℃, but no significant difference when the temperature rose to 35℃.

10℃、25℃时,花柱S-RNase抑制自花花粉萌发和花粉管生长的程度极显著大于抑制异花花粉的程度;花柱S-RNase降解自花花粉管RNA的程度也明显大于降解异花花粉管RNA的程度;温度升高到35℃时,自花和异花间花粉萌发和花粉管生长被花柱S-RNase抑制的程度不存在显著性差异;花柱S-RNase降解自花和异花花粉管RNA的程度也相似。

According to the results, the germicidal efficacy was influenced by the concentration of ethanol, concentration of Tryptone Soy Brothculture medium and contact time of bacatericide. The optimal treatment for inhibition of the growth of pathogenic bacteria bioflims by ethanol was: ethanol concentration of 70%, contact time of 40 min and in 0%TSB.

实验结果表明,乙醇浓度、胰酶大豆肉汤培养基浓度、作用时间均能影响抑制病原菌生物被膜,乙醇抑制生物被膜细菌生长的最佳条件为:乙醇浓度70%,处理时间40 min,TSB浓度为0。

Previous studies show that p33ING1b is involved in growth inhibition, apoptosis, chromatin remodeling, DNA repair, tumor suppression and cellular senescence, but its biochemical function and involvement in cellular senescence is still not fully uncharacterized.

p33ING1b是一个较晚发现的肿瘤抑制基因 ING1的主要表达形式,自从被成功克隆以后得到了广泛的研究,已有的研究表明,p33ING1b参与了细胞的生长抑制、凋亡、染色质重塑、DNA损伤修复、肿瘤抑制和细胞衰老等。

"Recent studies on the autoimmune origin of vitiligo also emphasize the probable implication of the immunosuppressive action of UV therapy in the repigmentation of vitiliginous plaques," the authors note."acrolimus, a new topical immunosuppressive drug developed for the treatment of atopic dermatitis, has shown some interesting results in treating vitiligo in 2 prospective studies."

研究人员指出,最近对於白斑自体免疫来源的实验,也强调了UV疗法的免疫抑制作用对於白斑再色素化可能的应用;Tacrolimus,一种新的局部免疫抑制药物,被开发用於治疗异位性皮肤炎,已经被两项前瞻性试验受瞩目的结果证实可以治疗白斑。

PCL can agglutinate many kinds of erythrocyte. The hemagglutinating activity of PCL on rabbit red blood cells was speelbeally inhibited by a few glycoprotein, but was not inhibited by all mono-, dl, oligo-and polysaecharides that have been tected.

PCL能凝集各种动物来源的红细胞,凝集兔红细胞的作用不能被已测试的单糖、双糖、和聚糖所抑制,仅被少数几种糖蛋白抑制。

The electric capacity coupling matching and inductance coupling match are two kinds of forms that conducted coupling, passing the analysis to constitute the mechanism and principle to them, putting forward repressing a few of their methods: Match the length that can adopt to let up to lead line and enlarge the line to be apart from to the electric capacity coupling ,.

电容性耦合和电感耦合是传导耦合的两种形式,通过对它们构成机理以及原理的分析,提出了抑制它们的几种方法,如对于电容性耦合可以采用减小导线的长度、增大线间距,平衡法,对干扰源和被干扰源进行屏蔽等方式进行抑制干扰;对于电感耦合可以用减小互感,局部形成耦合环,对干扰源进行屏蔽等方式来对干扰进行抑制。

The results showed that the three disparate treated substances have no visible antibacterial effect to E. coli , but a obvious effect to Staphylococcus aureus with inhibition of about 10 bacteriostasis units; have dissimilar effects to 10 kinds of fungi among the total kinds, and have a good inhibitory effect to Cytospora.coccodesHughes with fungistasis rate above 65%. After the gel filtration and activity detection, 50mM ammonium acetate was screened out as the more suitable eluents, the crudes were separated into strikingly unlike parts with obviously different antimicrobial activity.

结果表明,大蜡螟抗菌物质对大肠杆菌没有明显的抑制作用,而对金黄葡萄球菌抑制作用明显,抑菌活性单位在10左右;对10种植物病原真菌均有不同的抑菌活性,其中对苹果树皮腐烂病菌的抑制率达到65%以上;经过凝胶过滤分离,及对分离组分的抑菌活性测定,筛选出较合适的洗脱液为50mM乙酸铵,各分离组分抑菌活性差异明显,粗提物被分成了两个差异显著的部分。

Meanwhile,HDAC1 acetylation level is significantly increased.The overexpression of HDAC1 can promote erythroid cell proliferation and inhibits induced differentiation,the knockdown of HDAC1 or 2 inhibits erythroid cell proliferation and promotes induced differentiation.We also found that HDAC1 affects GATA1 mediated transcription activity.Thus our data revealed that HDAC1 may negatively regulate GATA1 mediated transcription and suggest that the dynamic regulation of GATA1 associated NuRD/MeCP1/HDAC1/HDAC2 complex may determine the onset of erythroid differentiation programs.Moreover,this study will eventually help to design new therapeutic approaches in treatment of leukemia.

研究发现在被诱导分化的小鼠红细胞白血病细胞中与GATA1相结合的HDAC1被乙酰化并失去活性,在血细胞中过表达HDAC1能够促进血细胞的增殖并抑制分化,反之在血细胞中敲除HDAC1或HDAC2能抑制血红细胞的增殖并促进分化,而且HDAC1能够负调控GATA1的转录活性,GATA1与NuRD/MeCP1复合体之间的动态调控,决定血红细胞分化的发生和发展,并为探索白血病的致病机理奠定了理论基础。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?