表达的

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

In addition, we presume that the role of different MMPs is distinct in various stages of EMs development. The difference of subjects which is resulted by the genetic difference of MMP-1 has the association with the risk of EMs, and the abnormal expression of protein may have relation with the development of EMs; The abnormal expression of MMP-3 has a very important role in the development of EMs; As a protein of endothelial expression, MMP-7 may participate in the development of EMs, endometrial cells with high-expression MMP-7 easily take place ectopic implantation in the blood of menstruate countercurrented and increase the risk of EMs; It is not confirmed in our study that there are the association of MMP-9 with the risk of EMs, but in view of other study, we will further evaluate the role of MMP-9 in the development of EMs.

且推测不同的MMPs基因在子宫内膜异位症发生和发展的不同阶段作用不同，MMP-1基因的遗传差异所导致的个体差异与子宫内膜异位症发病风险的个体差异相关，其蛋白的异常表达还可能与子宫内膜异位症的进展相关；MMP-3基因的异常表达在子宫内膜异位症的发展中起重要作用；MMP-7作为一个上皮性表达的蛋白，可能对子宫内膜异位症的发生起重要作用，带有MMP-7高表达的在位子宫内膜细胞更易在经血逆流中"异位"种植，增加个体子宫内膜异位症的发病风险；MMP-9虽然在本研究中没有被证实与子宫内膜异位症的发生和发展有相关性，但鉴于其它相关研究，有待于从其它方法证实MMP-9在子宫内膜异位症进展中的作用。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的：研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB／c小鼠和HBV转基因小鼠后，其免疫预防作用（接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律）以及免疫治疗作用（血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化）。

On the basis of Earth surface space using reference ellipsoid as height basis, map space using Gauss-Kruger projection plane as height basis, a viewpoint is proposed that map projection actually establishes corresponding relationship between the height bases of two different spaces above. From the viewpoint of projection and visible representation, the relationships among earth surface space, RS imagery space and map space are analyzed; the differences among different representations of earth surface space are identified theoretically; virtual reality is applied to the representation of land surface space, and a prototype of real 3D virtual reality system based on spatial information is developed. By means of digital simulation, virtual reality representation of land surface space in visual image space is realized.

在以参考椭球面为高程基准面表征地表空间、以高斯-克吕格地图平面为高程基准面表征地图空间的思想基础上，提出了地图投影实质上是建立两个空间的高程基准面之间关系的观点；从投影和可视表达的角度，分析了地表空间、遥感图像空间、地图空间之间的关系，从理论上区分了对地表空间的不同表达方式之间存在的差别；将虚拟现实技术应用于地表空间的表达，研制了空间信息三维虚拟现实系统原型，利用数字模拟技术在视像空间实现了对地表空间的真三维虚拟现实表达。

Asymmetric over-introgression of the indica alleles on chromosome 7 and many specific regions on other chromosomes in the japonica genetic background is consistent with the general trend observed in the RIL and F_2 populations of the same cross, suggesting a high genetic load in the japonica gene pool, and japonica genome experienced a very severe bottleneck by artificial domestication.2 Genetic background effect on QTL expression of grain weight and its related traitsGenetic background effect on main-effect QTL and digenic epistatic QTL mapping for GWT and its related traits including GL, GW and GT was dissected by a large set of reciprocal introgression lines and recombinant inbred lines derived from Lemont and Teqing in Beijing environment.

籼粳相互导入具有不对称性，粳稻向籼稻背景的平均导入频率与期望值相仿，相反籼稻向粳稻背景的平均导入频率大大高于理论预期，位于第7染色体和一些其它染色体的特殊区段存在籼稻等位基因的超导入现象，在来自相同亲本的重组自交系和F_2群体中也观察到同样的导入趋势，表明粳稻基因组具有较大的遗传负荷，粳稻品种在人工驯化过程中曾经历过一个非常剧烈的遗传瓶颈。2水稻籽粒相关性状的主效和互作QTL表达的遗传背景效应利用Lemont和特青培育的双向导入系和重组自交系群体，在北京环境下定位影响千粒重及其相关性状如粒长、粒宽和粒厚的主效和互作QTL，分别在特青背景导入系、Lemont背景导入系和重组自交系下检测到影响千粒重及其相关性状的主效QTL分别为33个、27个和13个，互作QTL分别为4个、8个和23个，其中仅有6个主效QTL(QGwt5，QGl1，QGl3b，QGl4，QGw7和QGw8)在籼粳两个不同背景下共同表达，3个主效QTL(QGwt5，QGwt7a和QGt5)在导入系和重组自交系中共同表达，分别占检测到影响这些性状QTL总数的11.1%和4.3%，未检测到在3个背景下相同表达的互作QTL。

There were 6 histidines linked to the N-terminal of targeted protein which was expressed by pRSETA-B7-2-PE40KDEL vector, which facilitated the purification process by using Ni-NTA affinity chromagraphy.

由于本研究构建的pRSETA-B7-2-PE40KDEL表达载体所表达的目的蛋白N端融合有6个组氨酸残基标记，这十分利于通过Ni-NTA金属鏊和层析来进行表达产物纯化。

Results P63 gene did not express in the pancreatic duct adenocarcinoma,but expressed highly in the adenosquamous carcinoma.

目的 研究P53、P63基因在胰腺肿瘤中的表达及其意义；P63基因表达与P53、增殖细胞核抗原、P16表达的关系及其意义。

We also use rank sum test to analyse the differe- nce between the light pre-eclampsia group and the severe pre-eclampsia group (P=0.000),the early onset pre-eclampsia group and the late onset pre-eclampsia group (P=0.003). All of the results give the same conclude that the differences within the three groups are significant (P.05). Conclusion: We conclude that HLA-G proteins are normally expressed in invasive trophoblasts and HLA-G proteins expression is defective in most pre-eclamptic placenta.We propose that trophoblasts lacking HLA-G proteins are vulnerable to attack by the maternal immune system.

结论HLA-G分子特异性分布于胎盘EVCT的胞膜及胞浆中，生理妊娠组、子痫前期组间差异性存在表明其表达量的下降与子痫前期的发病相关；轻、重度子痫前期组间显著差异表明HLA-G分子表达量的下降与子痫前期病情严重程度相关，早发型重度子痫前期患者胎盘组织中HLA-G分子的表达明显低于迟发型组，提示我们HLA-G分子表达的降低程度还与发病早晚有关。

Expression of recombinant mature arctic fox interferon gamma in bacterial system was confirmed by SDS-PAGE and Western blotting analysis.

应用原核表达系统高效表达北极狐g-干扰素成熟肽蛋白， SDS-PAGE和Western blotting分析表达的融合蛋白分子量约为19 kD，以不溶性的包涵体形式存在。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。