表达的

In order to investigate the role of ICAM-1 and VCAM-1 in the pathogenesis of glomerulonephritis, ICAM-1 and VCAM-1 expression were evaluated from both protein and gene level by in vivo and in vitro study, We have conducted (1) immunocytochemical analysis and in situ hybridization to examine the alteration in expression of ICAM-1 and it's relationship with interstitial infiltrating cells and TNFα; A total of 64 renal biopsies were classified in three groups according to the degree of cellular proliferation and infiltration in glomerulus;(2) indirect immunofluoresence and immunoblotting. methods to detect the VCAM-1 level both in renal tissue and in serum from the patients of lupus nephritis (17cases) and crescentic nephritis (4 cases);(3) Cell ELISA and northern blot technique to study the effects of TNFα and IL-1β on ICAM-1 and VCAM-1 surface expression and gene expression by cultured human mesangial cells .

为了探讨ICAM-1和VCAM-1在肾小球疾病中的作用，本文从蛋白质和基因两个水平，整体（研究ICAM-1时根据肾小球内细胞增生和浸润程度，将64例病人分为A.B.C三组）和细胞培养两个方面，做了如下工作，（1）利用免疫细胞化学和原位杂交技术观察了ICAM-1在64例肾小球疾病患者中的表达及其与间质浸润细胞、TNFα之间的关系；（2）利用间接免疫荧光和膜免疫印迹方法检测了VCAM-1在17例狼疮肾炎和4例新月体肾炎病人肾组织及血清中的表达；（3）利用细胞ELISA和Northern杂交技术研究了IL-1β或TNFα对体外培养的人肾小球系膜细胞ICAM-1和／或VCAM-1表面表达及mRNA表达的调节作用。

Accordingly, a new plasmid Cm1301 was constructed containing osGP fragment (-197～-56) from the osRACD promoter with the minimal promoter,-90 region of CaMV 35S which confers Gus activity detectably only in roots. In the transgenic plants containing Cm1301, GUS activity was detected mostly in the anther.

由于35S基本启动子是一个根特异表达的启动子，因此将包含osDP5区段的osGP片段（-197～-56）与35S基本启动子连接，构成植物表达载体Cm-1301并转化烟草，研究发现，osGP区段使gus基因在雄蕊中表达水平显著提高。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

The positve rates in serous carcinomas and borderline tumors were 39.3% and 55.6%, significantly lower than that in benign tumors and normal ovaries (P.05), but the statistical differences were not found between borderline tumors and serous carcinomas, benign tumors and normal ovaries.2 WWOX mRNA and protein expression in ovarin seruous tumors2.1 WWOX mRNA expression in ovarin seruous tumors The result of RT-PCR showed that WWOX mRNA expression were detected in 29 of 32 ovarian serous benign tumors, 12 of 16 ovarian serous borderline tumors, and 21 of 45 ovarian serous carcinomas respectively.

阳性条带密度扫描定量结果，卵巢浆液性癌中PTEN蛋白表达相对量为0.34±0.12比正常卵巢和浆液性囊腺瘤和交界性囊腺瘤明显降低(p.05)，其余各组间无统计学差异。3.2.2 PTEN蛋白FCM检测结果FCM结果，卵巢浆液性癌PTEN蛋白表达的FI值(0.908±0.023)明显低于正常卵巢、卵巢囊腺瘤和交界性囊腺瘤的FI值，差异有统计学意义(P.05)。3.2.3 PTEN蛋白免疫组织化学结果35例浆液性囊腺瘤、18例交界性囊腺瘤和56例浆液性癌中，分别有31例、15例和29例呈阳性表达。

To explore (1)whether the EPO is present in gastric carcinoma ;(2)the possible mechanism of its expression;(3) the role of EPO in tumor angiogenesis and its results, we investigated the expression of EPO, HIF-la in gastric carcinoma and marked EC with CD105( monoclonal antibody MS1290 )and CD34 on paraffinic slides. Analysis of the realationship between EPO MVDand HIF-la in human gastric carcinoma.

本实验检测人胃癌及癌旁组织中EPO、HIF-1a的表达，同时进行了小鼠抗人CD105单克隆抗体(MS-1290)标记和CD34标记，分析EPO与微血管密度及HIF-1a表达的关系，以期明了①胃癌组织中是否存在EPO的表达、②表达的途径和机制、③EPO与肿瘤血管生成的关系和可能的后果。

Terminal oxidative inhibitors, such as KCN, Salicylhydroxamic acid , antimycin A and NaN〓, can cut off electron transport chains of cytochrome pathway and alternative pathway, marked decreased BADH protein levels. Oxidative phosphorylation uncoupler 2, 4-dinitrophenol had a similar negative effect on BADH gene expression, and exogenous ATP and ADP increased the BADH levels. These results, in conjunction with the terminal oxidation results, indicated that ATP could be a critical component in BADH gene expression.

2.2 末端氧化抑制剂KCN、SHAM、抗霉素A和NaN〓能打断呼吸链，阻断电子传递，使BADH蛋白的表达量明显降低，说明末端氧化同样参与BADH基因表达的调节：末端氧化影响基因表达的实质就是ATP的影响，线粒体磷酸化解偶联剂DNP明显降低BADH蛋白的含量，而外源的ATP和ADP对BADH基因表达的正效应作用最大。

The expression of the unfused protein in the strain E.

将PoIFN-α1a插入到原核表达载体pGRW，转化大肠杆菌DH5α，得到的重组菌株经诱导有以包涵体形式表达的目的蛋白条带出现，表达量约10％。

Maybe it is a new member of b-galactosidase family. bin2a mrna is upregulated by androgen and also epididymis-specific, majorly in caput epididymides.

所以，bin2a可能是b-半乳糖苷酶家族的新成员。bin2a表达受雄激素上调，其表达的组织专一性也很高，只在大鼠附睾有表达，并主要在附睾头部。

In order to get the highly expressed recombinant NGF, the insect expression system was selected and the NGF gene with signal peptide was transected into insect cells.

为了获得高表达的重组NGF本文选择了昆虫表达系统将带有信号肽的NGF基因转染至昆虫细胞内，48小时后收取表达上清，蛋白电泳及活性检测。

There was a positive correlation between the expression of VEGF in glomeruli and 24 h urinary protein excretion.

结果 在先兆子痫大鼠中，尿液中VEGF含量（以尿VEGF/尿肌酐表示）升高且与肾脏局部VEGF的表达强度呈正相关，肾小球局部VEGFmRNA和VEGF蛋白表达均增强，且表达的强度与24h尿蛋白排泄量呈正相关。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。