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Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础,构建了原核表达载体PQE30a/FeSOD,经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后,再转化入大肠杆菌M15中,通过IPTG诱导,得到高效体外表达,经SDS-聚丙烯酰胺凝胶电泳检测,表达的目的蛋白占总菌体蛋白的37%,可溶性和包涵体两种形式均有存在,上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析,表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Results The experiment of immunohistochemical double-staining showed there were cardinal red granules in violet cytoplasm of VEGF-C positive TAM, and VEGF-C was weakly positive in intratumoral tissue, and strongly positive in peritumoral tissue.

结果 免疫组织化学双重染色显示:VEGF-C阳性表达的TAM中可见在紫黑色胞浆内有深红色颗粒存在,且VEGF-C在肿瘤内呈弱阳性表达,而在肿瘤周围间质的TAM中表达呈强阳性。

Objective To construct the eukaryotic expression vector for HPC2 for expression in HEK293 cells.

目的 构建在哺乳动物细胞中表达的HPC2真核表达载体,并分析其在HEK293细胞中的表达。

Objective To study the effect of human β-globin matrix attachment region on transgene expression in stably transfected CHO cells.

目的 研究MAR在CHO细胞中对转基因的表达调控作用,为建立稳定高效表达的CHO表达系统奠定基础。

In different embryo developmental stages, its expression was decline gradually . This result indicated that the survivin may play important role in oogenesis and early embryogenesis. One PABP gene was cloned from the subtractive cDNA library.

RT-PCR分析表明该基因主要在卵巢中表达,随着胚胎发育表达逐渐减少,这可能表明该基因在半滑舌鳎卵子形成和早期胚胎发育中起到重要作用。ePABP是特异的在卵巢和早期胚胎表达的Poly结合蛋白。

The recombinant proteins form inclusionbody in cytoplasm. Dissolve the inclusionbody with urea and refold it with metal affinity chromatography. The refold recombinant protein has certain antibacterial activity.

对表达产物进行复性,结果显示无论是融合表达的VHb-Fetidin还是非融合表达的Fetidin都无法用稀释法和尿素浓度梯度透析法成功复性,只有VHb-Fetidin可以通过金属亲和层析柱成功复性。

Those expression plasmid were respectively transformed into E.coli DH5a, thenthe expression strains were induced for 5h under 42℃, SDS-PAGE confirmed that interestprotein was expressed by pBV220-R- IFN-γ_m strain and was 16kd in size. Its contentaccounted for 38.6%of the thalline protein.

分别转化大肠杆菌DH5α,42℃诱导表达4h,经SDS-PAGE电泳鉴定,原核表达质粒pBV220-R-IFN-γ_m表达的目的蛋白大小为16Kd,占细菌总蛋白的38.6%。

We found for the first time that the epithelium of bronchus of normal broilersexpressed iNOS mRNA.iNOS activity in the epithelium of bronchus of preascitic andascitic broilers were higher than normal broilers,but there was no iNOS activity inpulmonary vessels of all the three types broilers.The results indicated that the increasedNOS expression in pulmonary vessels of preascitic and ascitic broilers was not iNOS.

首次发现在肉鸡肺动脉高压发展过程中,肺血管NOS的表达是增加的,尤其是正常鸡无NOS表达的肺小血管内皮和平滑肌在腹水鸡却呈高水平表达,且NOS活性增加的肺小血管也同时存在管壁平滑肌层增厚或无肌型血管肌化的结构重建现象,结果提示NOS表达增强可能与肺血管中膜增厚有关。

Three genes specified expressed in hybrids and one gene over-expressed in hybrid were found. By cloning, sequencing and BLAST analysis, one specific expression cDNA band was cytosolic isocitrate dehydrogenase gene, the other two were new genes which function were unknown, and the over-expressed cDNA band was proteosome subunit p112 gene.

发现了 3 个杂种鸡特异表达的基因和 1 个杂种鸡表达增强的基因,进一步克隆、测序和比对分析初步表明:杂种特异表达的 cDNA 片断分别为鸡细胞质异柠檬酸脱氢酶基因和 2 个未知功能的新基因;杂种表达增强的 cDNA 片断为鸡蛋白酶体亚基 p112 基因。

To this end,we used the Transwell system to select two highly invasive subcell lines SNU5-P3 and PAMC82-P3 from minimally invasive parent cells,and compared gene expression in paired cell lines wit...h high and low invasive potentials.38 genes were commonly upregulated in the two highly invasive subcell lines.

通过全基因组芯片比较SNU5-P3和PAMC82-P3与其亲本细胞的基因表达谱,获得了38个在两张芯片共同上调表达的差异基因,其中16/38(42%)已报道是与侵袭转移功能相关的基因。LOXL2是一个在两张芯片共同上调表达的基因,通过生物信息学分析和细胞表达谱分析,表明LOXL2可能是胃癌侵袭转移密切相关的功能基因。

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