表达的

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，"PMN-EC"相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

The Heaven and the Way is the both the changeableness of the universe and the thought to pursue harmony in the world; The Humanity is the thought to pursue harmony in the conflict society by culturing mind, which means a real person must be of disenchantment in thought and self restraint in behavior, and the society is of solicitude for human, all that is the ZhongYong; The Unifying and the Changing is the harmony in evolvement, the essence of the universe and thought;as a thought, it is the Way of communication between human and nature or society; The one is ultimate perfection but that means death, only in its own transcending it becomes the the primary chaos,namely ultimately the Chinese thought.

本文中使用的中国思想区别于一般使用的"中国传统思想"，中国思想具有的自身的本体性意义，它不同于对一般的思想观念的研究，不是研究它所表达的内容的特点和性质，也不是指某种具体思想观念的产生和发展的演变，而是指思想自身作为动力性本质的本体性存在过程；"中国传统思想"则具有它所指称的历史内容，是指中国文化历史进程中具体的思想脉络，当然"中国传统思想"也有概念自身特定的研究意义，但那是建立在全部历史内容的基础上，用来表达中国历史自身的精神特质。

Compared with classical cytotoxic T lymphocytes , NK cells are superior in the following aspects: NK cells are among the first cell types to be activated after intracellular pathogen stimuli, the activity of NK cells peaked between 1 to 3 days, retained during the first 7-10 days, and the T cells response emerged only after 7 days when the NK cell responses begin to decline; though comprising of only 10％-15％ of peripheral lymphocytes, NK cells mobilize most of the cell populations in a strong and high level response to different invasions, and act as key factors at early defense before a specific immune response could mount, while only certain clones were involved in T cell response; NK cells kill virus-infected or malignant transformed cells without pre-sensitization and without restriction by major histocompatibility antigen, which is usually a mechanism of immune escape to T cell recognition by virus-infected or tumor cells, thus NK cell in complementary with T cells play crucial roles in tumor and virus eradication.

NK细胞以其强大的细胞毒活性为特征，与细胞毒性T细胞相比，NK细胞具有如下特点：NK细胞虽然只占外周血淋巴细胞的10-15％，但是对刺激性因素产生应答的过程十分迅速，一次可以调动大多数细胞共同参与，而不是几个克隆的活化，免疫应答的强度高；病毒感染或恶性转化细胞的一个主要特征是细胞表达的MHCⅠ类分子下降或消失，并借助这一机制逃避特异性T细胞应答的识别，NK细胞介导的杀伤活性无需抗原刺激，不受MHCⅠ分子表达的限制，从而与T细胞应答互为补充发挥免疫防御的功能；NK细胞的免疫活性可以被多种细胞因子上调，其中IFNα，IL-2，IL-12，IL-15和IL-18具备更强的作用；NK细胞具有强大的细胞因子分泌功能，对于启动T细胞特异性应答必不可少。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，&PMN-EC&相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

2 Aids to Interpretation In these Terms and Conditions and in the Contract Details, unless the contrary intention appears; words denoting the singular include the plural and vice versa; a reference to any one of an individual, corporation, partnership, joint venture, association, authority, trust or government includes any other of them; the headings are for convenience only and do not affect interpretation; a reference to any instrument (such as a deed, agreement or document) is to that instrument (or, if required by the context, to a part of it) as amended, notated, substituted or supplemented at any time and from time to time; a reference to a Party is a reference to a party to the Agreement and includes that Party's executors, administrators, successors and permitted assigns; a reference to a clause or attachment is to a clause (including sub-clause, paragraph, sub-paragraph or further subdivision of a clause) or attachment of or to these Terms and Conditions; a reference to any legislation or legislative provision includes any statutory modification or re-enactment of, or substitution for, and any subordinate legislation under, that legislation or legislative provision; where an expression is defined, another part of speech or grammatical form of that expression has a corresponding meaning; a reference to "US$" or "US Dollars" is a reference to the currency of the United States of America.

1.2艾滋病的解释在这些条款和条件，并在合同中的细节，除非相反的意图似乎；的话指的奇异包括复数，反之亦然；提及的任何一个个人，公司，合伙，合营，协会，主管部门或政府的信任，包括任何其他人；标题只是为了方便起见，并且不影响解释；一个参考的任何文书，是该文书的（或者，如果所要求的背景下，一个它的一部分）修订， notated ，取代或补充，在任何时间，从不时；一个参考一党是一个参考，以协议的当事方和包括党的执行者，管理者，继承人和允许的受让人；一个参考的条款或附件，是一条第（包括子第，段，分段或进一步细分的一个条款），或扣押，或这些条款和条件；一提到任何立法或立法条文包括任何法定修改或重新制定的，或替代，以及任何附属法例下，即立法或立法条文；在1表达的定义是，另一部分言论或语法形式的表达有一个相应的意义；中提到&美元&或&美元&是一个参考的货币美利坚合众国。

The fourth was astounding.I saw a woman disguised in her maid's clothes hear her husband speak the first tender words he's offered her in years.Simply because he thinks she is someone else.I heard the music of true forgiveness filling the theater conferring on all who sat there, perfect absolution.God was singing through this little man to all the world.Unstoppable.

那段二重唱是很美丽，但我还是喜欢第四幕里gente，gente， all'armi， all'armi那一段，那是我在米洛斯福尔曼的传记电影莫扎特里感染到的，在最后一幕里费加罗的未婚妻装扮成女仆然后她的未婚夫-当然就是费加罗当着她的面在说他有多么的爱他的未婚妻，因为他重来没有尝试这表达，而他今天表达的只应为他并不知道女仆就是她的未婚妻。

METHODS: Spleen cells of 1, 2, 4, 6, 8, 10, 12, 16, 20 and 24 h in the coculture system were observed and the expression of RNA editase was detected by semiquantitative PCR and was contrasted with the mouse spleen cells in a singleculture system. The mouse model with the immune system restrained by FK506 was made, its spleen cells were cocultured with stimulated cells and then the effect of FK506 on RNA expression of editase was detected by semiquantitative PCR.

观察共培养1，2，4，6，8，10，12，16，20及24 h的小鼠脾细胞，用半定量PCR的方法检测RNA编辑酶表达的变化，并以相同时段单独培养的小鼠脾细胞RNA编辑酶的表达情况进行对照；制备免疫功能受FK506抑制的小鼠模型，取其脾细胞与刺激细胞进行相同时段的共培养，用半定量PCR的方法检测FK506对脾细胞RNA编辑酶的影响。

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示，Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域；与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠，在RPE未检测Kir2.1蛋白的免疫活性存在；Kir4.1蛋白集中分布于Müller细胞的内侧突起，与GS蛋白双重标记显示其分布特性重叠，RPE未检测Kir4.1蛋白的免疫活性存在；Kir7.1主要分布于RPE游离面及其突起的全长，与特异性标记RPE突起的Ezrin蛋白完全重叠，Na〓-K〓ATP酶在不同部位的RPE表达不同，Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

RESULTS:Berberine inhibited IL-1, TNF-induced HUVEC adhesion for PMN when pretreated HUVEC and antagonised IL-1, TNF-induced upregulation of ICAM-1 on HUVEC. Meanwhile, TNF-stimulated PMN adhesion for HUVEC and CD18 upexpression on PMN was diminished in the presence of berberine.

结果：小檗碱与IL-1或TNF共同处理HUVEC，能抑制IL-1、TNF诱导的PMN与HUVEC间的粘附增强，且能下调由IL－1、TNF诱导的HUVEC表面粘附分子ICAM-1表达的增高；小檗碱与TNF共同处理PMN，能抑制TNF诱导的PMN与HUVEC的粘附增强，亦能降低TNF诱导的PMN表面粘附分子CD18的表达。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。