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This thesis conclude 4 parts: 1 、 The change and effecting factors of plasma adiponectin level in old subjects with abnormal glucose tolerance; 2、 The distribution of adiponectin receptors in Wistar rat; 3、 The effect of fat-fed and pioglitazone on mRNA expression of adiponectin with its receptors...

本研究共包括以下四部分内容:1、老年糖耐量异常人群中脂联素的改变及其影响因素;2、脂联素受体mRNA在Wistar大鼠体内的分布;3、高脂饲养以及吡格列酮对大鼠脂肪组织脂联素及其受体表达的影响;4、吡格列酮对不同浓度脂肪酸培养的HepG2细胞脂联素受体mRNA表达的影响。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO,在斑马鱼受精卵的单细胞期,进行不同浓度的显微注射,并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验,验证tbx2-MO抑制tbx2基因表达的有效性和特异性;基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA,来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

The study on the expression of TGF β1 gene in Escherichia coli, not only can provide great amounts of TGF β1 for its clinical and basic research, and also will bring some new ideas and means for the research of other dimeric growth factors.

开展TGFβ1基因在大肠杆菌中表达的研究,不仅有可能获得大量具有生物学活性的TGFβ1产品,从而为TGFβl深入的基础和临床研究提供可能;而且可能为其他双体生长因子的原核表达提供新的思路和方法。

The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell comprising a gene coding for the protein, the gene is expressible in said cell, cultivating the cell under conditions where the gene is expressed, isolating the protein from the cell without reducing it, and subjecting the isolated protein to a folding treatment.

本发明涉及一种产生功能性免疫球蛋白超家族蛋白的方法,此蛋白质在有功能时具有至少一个二硫键,此方法包括如下步骤:提供一种包含编码此蛋白质的基因且此基因可在其中表达的细菌细胞;在使此基因表达的条件下培养此细胞;从该细胞中分离出此蛋白质而不还原它;以及使分离出的蛋白质经受折叠处理。

Low expression of PTEN had a significantly positive correlation with the degree of differentiation, infiltrative deepness and lymph node metastasis of carcinoma, suggesting that PTEN plays an important role in carcinogenesis and progress of esophageal cancer.

结论PTEN蛋白表达的降低在食管癌的发生发展中可能起重要的作用,PTEN蛋白表达的检测有望成为食管癌辅助诊断和判断预后的参考指标之一。

It shows that Chp has relation to the extent of ASO. The expression of Chp DNA depends on the lesion of artery, the distal artery of obliteration and the extent of lesion in laterad artery.3. The clinical significance of the Chp detectionThe macrolide antibiotic is a kind of drug that is used to treat Chp infection.

2· 二人hp与ASO严重程度的关系本研究表明,ChPDNA的阳性表达率随ASO的严重程度而升高,说明ChP与ASO病变程度相关,即ChpDNA表达的变化取决于动脉本身病变、闭塞远端血管及侧枝血管内皮细胞的损伤程度。

Methods: The expression level of NOS and NGF was detected in pterygopalatine ganglion of the allergic rhinitis rats using histochemieal and immunofluorescence staining methods respectively.

建立AR大鼠模型,用HE染色方法观察鼻黏膜内腺体的变化;用组织化学的方法观察翼腭神经节内NOS表达的变化;用免疫荧光的方法观察翼腭神经节内NGF表达的变化。

After being induced to differentiate in vitro, cells with various morphologies showed β-gal activity, including nerve cells, neuroglial cells, epithelial cells. We also detected β-gal activity in a wide variety of tissue elements in solid tumors made by injecting the MC15 and MA34 cells into syngenic mice. Then we produced chimeric embryos by injecting the MC15 cells into Kunming blastocysts and recovering the embryos at various developmental stages.

SiCMVIE(Simian Cytomegalovirus Immediately Early)启动子是一个强启动子,本文首次将SCMVIE启动子应用到ES细胞途径上制作嵌合鼠,并通过lacZ基因在ES细胞和早期嵌合胚胎中的表达,表明该启动子是一个能在小鼠多数组织表达的启动子,这与1993年Peppel等人用原核显微注射法获得的转基因小鼠上所得的有关结果是基本上是一致的。

The proposal of"womanism"represents the maturation of her black female consciousness, which is embodied both in the ideology and in the art of her writings.

这种意识一方面体现在作品的创作思想中,另一方面体现在作品的创作艺术中,沃克文学创作的特征就在于她致力于表达的黑人女性意识和与此相关的文学表达方式。

He accomplished this by getting rid of the irons with which his wrists and ankles were shackled, cutting through a double grille of oak and iron bars, descending twenty-five feet by a knotted sheet and blanket, and climbing a twenty-two-foot wall.

翻译长句时,首先要弄清原文的句法结构,找出主句和从句,以及它们之间的逻辑关系,同时,还要从词、句的字里行间捕捉作者所表达的思想、感情、笔锋、神韵,然后再按汉语的表达习惯加以翻译。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。