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Meanwhile, in order to explore the stability of gene expression and the properties of gene-modified bone marrow cells further, we have observed the capability of reconstituting hematopoietic system of gene-modified and the expression of transduced gene in vivo by using of BMT to engraft lethally irradiated mouse with the gene-modified bone marrow cells.

同时,为进一步探讨转导细胞基因表达的稳定性以及生物学特征,本实验通过骨髓移植的方法,将基因标记骨髓细胞移植给受致死量照射小鼠,观察了基因转导细胞重建体内造血的能力,以及造血重建后外源基因在脾结节及骨髓细胞的表达。

But there are no correlations between gene expression and gene expression specificity or gene expression level.

有趣的是,基因的组织特异性和基因的表达量与基因表达的进化则可能没有相关性。

RESULTS Expression rate of MHCI antigen of cultured smooth muscle cells and fibroblast was(88.7±4.3)%,(84.8±2.0)%respectively;and that of the revived smooth muscle cells and fibroblasts of aortic homografts after cryopreservation was(89.4±3.5)%,(81.9±2.7)%respectively.

结果 正常培养的大鼠主动脉平滑肌细和成纤维细胞MHCⅠ类抗原表达的阳性率分别为(88.7±4.3)%和(84.8±2.0)%;液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHCⅠ类抗原表达的阳性率分别为(89.4±3.5)%和(81.9±2.7)%。

RESULTS Expression rate of MHCI antigen of cult ured smooth muscle cells and fibroblast was (88.7±4.3)%,(84.8±2.0)%respectively; and th at of the revived smooth muscle cells and fibroblasts of aortic homografts after cryoprese rvation was (89.4±3.5)%,(81.9±2.7)%respectively.

结果正 常培养的大鼠主动脉平滑肌细和成纤维细胞MHC Ⅰ类抗原表达的阳性率分别为(88.7±4.3)%和(84.8±2.0)%;液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHC Ⅰ类抗原表达的阳性率分别为(89.4±3.5)%和(81.9±2.7)%。

The expressions of VEGF in rats' skeletal muscles induced by acute exercise and/or hypoxia belong to the effect of immediate-early, with existing intensity-threshold, which recovery speed is inversely proportional to the expression amplitude;"training low-living high" may be able to enhance the adaptation of skeletal muscles to sports.

急性运动和/或低氧诱导的大鼠骨骼肌血管内皮生长因子的表达属早期速发效应,且存在强度阈值,其恢复速度与表达的幅度成反比,低练高住可能能增强骨骼肌对运动的适应。关键词:血管内皮细胞生长因子;低氧;低练高住;运动强度

They expressed prominently in the forebrain, mid-hindbrain boundary, branchial arches, somites, limb bud and tailbud of mouse embryos. These expression profiles indicate that Fgf8, Spry2 and Sef belong to a synexpression group and suggest that these genes may functionally interact during embryonic development.

在小鼠8.75天胚胎上(E8.75),这些基因在前脑,中脑和后脑的交界处,鳃弓,体节及尾芽等处有明显的表达,这些表达的相似性提示了它们可能在胚胎早期发育过程中的相互作用。

The construction of A83 eukarotic expression vector will play an important pole in researching the effect on HBeAg expression and disease mechanism when A83 mutation in HBV signal peptide reg

HBV A83点突变真核表达载体的构建为体外研究该点突变对HBeAg表达的影响以及HBeAg阴性的乙型肝炎病毒感染的致病机理奠定基础。

For the E. coli lac operon, write the partial diploid genotype for a strain that will produceβ-galactosidase constitutively and permease by induction.

以大肠杆菌的乳糖操纵元为例,请写出一菌株的部分二倍体基因型:β-半乳糖苷酶是组成型表达的,透过酶是诱导表达的

A comparative genome analysis was conducted on short-day induced gene expression to determine the increase of transcript abundance during senescent process in soybean plants.

对大豆短日照诱导的基因表达差异进行了比较基因组分析,在细胞分子水平上从基因差异表达的角度研究短日照诱导衰老过程中基因转录物丰度的变化,探索大豆衰老的复杂机制。

Objective To study the mechanism of the inbitory effect of matrine on central nervous. Methods In the present study, incubation of postnatal Wistar rat cortex neurons in culture with matrine at 0.5, 1.0, 2.0mmol/L was carried out to have knowledge of the effect of matrine on the expressions of GABAR.

目的 观察苦参碱对鼠原代培养皮质神经元γ-氨基丁酸受体蛋白表达的影响,探讨苦参碱抑制中枢的作用机制方法对Wistar新生大鼠原代培养皮质神经元分别给予剂量 0.5、1.0、2.0mmol/L培养基的苦参碱进行药物干预,4h后用细胞免疫组化方法测定GABAR的蛋白表达。

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