表达的

The hyperexpression of Survivin in tissue can reflex the biological behavior of HCC. The specificness of survivin protein expression make it to be new target of tumor diagnosis and treatment.

Survivin基因蛋白在HCC组织中过表达是反应HCC生物学行为的有效指标，Survivin基因蛋白表达的特异性有望成为HCC基因诊断与治疗的靶点。

The expression changes of ICAM-1 and p65 mRNA level were detected by reverse transcription-polymerase chain reaction and the expression of p65 protein in AR42J cell lines were detected with Stept Avidin-Biotin complex respectively, and the changes of amylase in culture medium with chromatometry.

逆转录－多聚酶链反应半定量法观察细胞间黏附分子(ICAM-1)和p65亚单位mRNA表达的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达；人工碘比色法观察培养液上清中淀粉酶的活性改变。

The expression changes of TNF-α and p65 mRNA expression were detected by reverse transcription-polymerase chain reaction, the TNF-α level in the supernatant was determined by ELISA, and the expression of p65 in AR42J cell lines was detected by Stept Avidin-Biotio Complex.

逆转录－多聚酶链反应半定量法观察肿瘤坏死因子/p65亚单位mRNA表达的变化；ELISA法检测TNF-α在细胞上清中质量浓度的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达。

Total RNAs from the leaves treated 24hrs with 430mM NaCl and controlwere extracted in wild barley Hordeum brevisubulatum (Trink. Link..The difference of mRNA transcriptions was detected using suppression subtract hybridization.

本研究以野生二棱短芒大麦为试材，运用SSH的方法比较了正常培养和430mM NaCl盐溶液胁迫处理24hrs基因转录水平上的差异，克隆出差异表达的cDNA片段449个，构建了差异表达cDNA文库。

This experiment was designed to explore the effect of glutamine/ lipopolysaccharide on expression of HSP70 in tissues of Trionyx sinensis and the relation between the function of Gln and it induce the expression of HSP70 in tissues. Twenty-four healthy Trionyx sinensises with similar weight were randomly divided into four groups.

本试验通过研究谷氨酰胺/脂多糖对中华鳖肝脏、脾脏和肠道组织热应激蛋白HSP70表达的影响，考察Gln对中华鳖免疫应激的保护作用与其诱导组织HSP70表达的关系。

Furthermore. the bilirubin oxsidase activity was detected in the supernatant of cell lysates from IPTG induced culture of E. coli containing pEI-3a/BOX These results demonstrated that bilirubin oxidase cDNA from Myrothecium Verrucaria has been successfully expressed in E. coli.

这些结果表明，疣疱漆斑菌胆红素氧化酶cDNA首次成功地在大肠杆菌中获得了表达，所表达的可溶性产物具有与天然蛋白质基本相同的分子量、相似的免疫性和酶催化活性

Methods The ARPE-19 cells were put into normal and hypoxic chamber respectively.After 0hr、8hr、24hr、48hr,the proliferation of ARPE-19 cells was evaluated by[3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromid, MTT]-test.

第一部分缺氧人视网膜色素上皮细胞株ARPE-19表达特性目的观察缺氧对人视网膜色素上皮株ARPE-19的细胞形态、增殖以及对其分泌的血管内皮生长因子和色素上皮衍生因子表达的影响。

The connotation of such expression should be expurgated, the protection subject and object should be clarified,and the protection model should be designed.

这需要廓清民间文学艺术表达的内涵，明确保护的主客体，设计合理的民间文学艺术表达保护模式。

However, an efficient expression system is not limited to secreting protein, keeping the ability of genetic and replication, it also has high expressive element.

当然，一套好的表达系统不仅要能分泌蛋白质，保持遗传和分裂的稳定性，还应该有一些高效表达的元件。

Out of 20 clones integrated with ibeB, two clones were investigated in detail.

对其中的2个进行插入基因表达的鉴定，为ibeB基因表达的阳性克隆。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。