表达的

From the calculation of two different similarity measures—Pearson correlation coefficient and Spearman correlation coefficient, we found that about 94 percent of gene expression regulatory pathways are correlatively expressed in more than 4(including 4) sets of expression profile data, and it presents additional evidence for the correlation between gene function and its expression in the view of gene expression regulatory pathways.

通过Pearson相关系数和Spearman相关系数两种相似性测度的分析，我们发现有94%（15条）的基因表达调控通路内的基因在大于等于4套的表达谱数据中是共表达的，以上结果从基因表达调控通路的角度，证实了基因功能与基因表达之间存在着一定的相关性。

Whole protein patterns during early stage of gynogenesis were first studiedthrough 2D electrophoresis. 11 specific protein dots were found, among which3(A1 ～A3 )were suggested to be the kind of inverse function factors which suppressgenes activating megagamete division, 8 (Bl～B8)were suggested to be closelyrelated with megagamet division activation.

通过双向电泳技术研究了黄瓜雌核发育早期的特异蛋白质表达，并发现了11个特异多肽点，其中3个(Al～A3)可能是开启分裂基因表达的一类反式作用因子，起抑制该基因表达作用。8个(B1～B8)可能是启动雌配子分裂密切相关的基因表达产物。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

ERα is the major subtype of estrogen receptor in vaginal tissue of rats. ERα and ERβ expressions are down-regulated in the vaginal tissues after ovariectomy. Our findings show that estradiol valerate can up-regulate the expression of ERα but ERβ.

ERα是大鼠阴道优势表达的雌激素受体亚型；去势后阴道ER亚型表达下调，以ERβ明显；戊酸雌二醇可明显上调去势大鼠阴道ERα的表达，但不上调ERβ的表达。

Observation with transmission electron microscope showed that interalveolar septa were widened with increased amount of collagen in the MP infected rats while there were no obvious abnormalities in the other two groups.(2) Positive expression of bFGF mRNA were found in alveolar walls of MP infected rats. No expression of bFGF mRNA was found in control animals. In the rats infected with MP but treated with erythromycin positive expression of bFGF mRNA was found to be scarcely distributed in alveolar walls.

2感染组动物肺泡壁见明显的bFGF mRNA阳性表达颗粒，对照组基本未见bFGF mRNA阳性表达，感染加红霉素治疗组仅见散在分布的少许bFGF mRNA阳性表达；定量分析结果表明，感染组bFGF mRNA阳性表达的积分吸光度为41.32±10.44，与对照组(0.30±0.13)和感染加红霉素治疗组(6.03±2.41)比较，差异有显著性( P ＜0.01)。

With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.

随着诱导时间的延长，表达的融合蛋白产量逐渐增加，表达蛋白总量最高可达到菌体总蛋白的39.14％；在15℃、25℃和37℃三个不同的诱导温度下，均能诱导产生融合蛋白，而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加；在菌体的各个部位中，融合蛋白主要在细胞质中以可溶的形式进行表达，有少量在外周质中表达或以包含体形式在细胞质中表达。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Bin2a mRNA is upregulated by androgen and also epididymis-specific, majorly in caput epididymides.

Bin2a表达受雄激素上调，其表达的组织专一性也很高，只在大鼠附睾有表达，并主要在附睾头部。

Resells The expression of Survivin in ectopic lesion of adenomyosis group was significantly higher than that of eutopic endometrium in adenomyos

结果生存素在研究组异位病灶中广泛表达，表达的强度显著高于其在位内膜和对照组子宫内膜，增殖期高于分泌期；研究组在位内膜的生存素表达也高于对照组内膜；无论是研究组在位内膜还是对照组子宫内膜中生存素的表达均不随月经周期变化。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了