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In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection.

本研究采用RT-PCR和Western Blotting技术,检测正常培养和感染HCMV的神经胶质瘤(U251)细胞NGF的表达,探讨人巨细胞病毒对神经胶质瘤细胞内源性神经生长因子表达的影响。

In this dissertation we present our results on the regulatory mechanism of expression of nodulation genes, mainly focusing on the bacterial RNA polymerase and transcriptional factors.

这些基因的表达受到了严格的调控。本文中我们从RNA聚合酶和转录调控因子两个方面研究了结瘤基因表达的调控机制。

ObjectiveTo investigate the expression and correlation of survivin and matrix metalloproteinase 9(MMP-9) in cholangiocarci- noma,and evaluate their correlation with invasion and metastasis in cholangiocarcinoma.

小 目的研究存活素及MMP-9在胆管癌组织中的表达情况及其相关性,了解胆管癌组织中存活素及MMP-9表达的相关性及其与胆管癌浸润转移的关系,并讨论其临床意义。

The results of preliminary activity identification indicated that this recombinant fusion protein can specifically kill CD28 positive human T lymphoma cell lines Jurkat, but have none effects on CD28 negative human leukemia cell lines Raji.

初步的活性测定显示,该重组融合蛋白能有效特异性地杀伤高表达CD28的人T淋巴瘤细胞系Jurkat,而对CD28阴性表达的人白血病细胞系Raji则无任何杀伤。

Among 55 gastric carcinoma tissues by means of operation, the positive expression rate of Fas was 41.18% in 34 cases with lymph node metastasis and 71.43% in 21 cases without lymph node metastasis.

通过对不同病理类型,有无淋巴结转移和胃癌术后是否达到5年生存期的胃癌组织中P27、Fas表达以及二者之间表达的相互关系的研究发现:1。

Computer image analysis was used to measure the average optical density of synaptophysin.

方法采用免疫组织化学方法,观察突触素在不同周龄的人胎儿海马中的表达水平,利用计算机图像分析技术测量不同周龄阶段海马突触素表达的平均光密度。

The permillage of the positive Cardiomyocytes of the expression of Bcl-2 protein began to increase at 1h group and then dropped gradually ,achieved the least at 4h , increased to the normal gradually at 8h and higher than the normal at 12h.

3阳性表达的Bcl-2蛋白主要也聚积于胞浆;心肌细胞Bcl-2蛋白表达阳性的细胞千分率在冠脉结扎后1h先略升高,然后逐渐下降,到4h时降至最低水平,之后逐渐上升,8h时已上升至稍高于假手术组,到12h时达到比假手术组还高的水平。

The expression level of integrin β1 on the surface of K562 cells was evaluated by flow cytometry; the growth of K562 cells treated with phycocyanin was measured by MTT assay; the expression level of FAK mRNA was analyzed by relatively quantitative RT-PCR after four-day culture of K562 cells with phycocyanin of 40 μg/ml,80 μg/ml and 160 μg/ml,respectively.

本 研究观察不同浓度藻蓝蛋白对K562细胞增殖的影响,并检测藻蓝蛋白处理后细胞表面整合蛋白β1表达和胞内黏着斑激酶基因表达的变化,探讨整合蛋白β1在藻蓝蛋白抑制K562细胞增殖中的可能作用机制。

Objective To evaluate heat shock protein 72 (HSP72) mRNA expression in myocardium of non cyanotic patients of congenital heart disease during cardiopulmonary bypass surgery for correction of congenital heart disease, to investigate if tetramethylpyrazine pre-conditioning can modify the expression of HSP72 during CPB ,and if TMP pre-conditioning can attenuate CPB-induced myocardial ischemia reperfusion injury through a self-protective mechanism.

目的:研究体外循环前后非发绀型先天性心脏病矫治术患者心肌组织中热休克蛋白72(HSP72)的表达和川芎嗪预处理对其表达的影响,并探讨川芎嗪预处理的心肌保护机制。

Objective To study the mechanisms and treatment of ischemia /reperfusion injury, expression of intracellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were measured, the effect on suppression of ICAM-1 and VCAM-1 by the pyrrolidine dithiocarbamate were investigated.

目的 为研究心肌缺血-再灌注损伤的机制和治疗途径,检测血管内皮细胞缺氧/再给氧后细胞间黏附分子- 1(intracellular adhesion molecule1,ICAM- 1)和血管细胞黏附分子- 1(vascular cell adhesion molecule1,VCAM-1)的表达,探讨抗氧化剂吡咯烷二硫氨基甲酸酯(pyrrolidine dithiocarbamate,PDTC)对血管内皮细胞表面细胞黏附分子表达的抑制作用。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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