表达的

The expressions of DNA-PKcs and Id2 in SP cells were strikingly stronger than those in non-SP cells, The localization of DNA-PKcs in SP cells was in whole nucleus but that of the protein in non-SP cells was only in nuclear membrane and nucleoplasm nearby.

SP细胞DNA-PKcs表达的阳性强度明显高于非SP细胞；SP细胞Id2表达的阳性强度明显高于非SP细胞；SP细胞的p16表达的阳性强度明显低于非SP细胞；SP细胞FHIT的表达为阴性或微弱阳性；SP细胞NFκB/p65表达的阳性强度明显低于非SP细胞。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

The research work involving the expression manner of doxA gene in S. lividans TK24 with plasmid pYG57 implied that the promoter PermE may control its expression constitutively, the time for maximum expression emerged from 48 to 60 h after inoculation and cultivation and the expression level was kept relatively stable, furthermore, the recombinant hydroxylase existed mostly in mycelium cell but little in broth.

而对Tm4（pYG57）菌株中doxA基因表达方式的研究表明， dd基因在 PermE启动子控制下可能是一种组成型控制表达，其表达量在接种后培养到 48－60 h左右最高并且维持相对稳定，并且表达的柔红霉素 Cl4羟化酶主要存在于菌丝体细胞内，很少分泌到细胞外。

It was also found that some genes which were expressed at high level in heterotic hybrid were underexpressed or expressed at low level in nonheterotic hybrid.

杂种Ⅰ与双亲间基因的差异表达类型较杂种Ⅱ丰富，且发现许多杂种Ⅰ高水平表达的基因在杂种Ⅱ减弱表达或偏低亲表达。

Opportunistically expressed (usually at the basal levels of transcription), but still permit the continued expression of genes that are regulated by strong promoters/enhancers (such as housing-keeping gene), the expresison of these genes might play a key role in the embryonic development; 5 The sum of these two opposing processes-activation and repression-would dictate the final pattern of gene expression that manifests itself as a dramatic reprogramming of gene expression during the maternal-to-zygotic transition and support further embryonic development.

这些基因的表达对于胚胎的后继发育可能是至关重要的；5）通过胚胎基因表达的激活与抑制：这两个作用相反的机制依不同时间顺序协同作用于胚胎基因，从而在指导胚胎发育的控制权由母源性表达产物移交到胚胎特异的基因表达产物的过程中&再程序化&胚胎基因的表达，形成特定的表达模式，以支持胚胎的后继发育。

Levels of expression significantly increased after day 4 posttransplantation and persisted through the whole course of rejection, but levels of expression were obviously lower in the peripherial blood than those in portal vein blood. In contrast, the control group showed no expression of mRNA for perforin and granzyme B in portal vein and peripherial blood.

结果 急性排斥肝移植组术后3天门静脉和外周血中均有穿孔素和颗粒酶B基因mRNA表达，4天后表达明显增强，并贯穿整个排斥反应的全过程，但外周血表达的强度明显较门静脉血为弱，同基因对照组术后14天内无穿孔素和颗粒酶B基因表达。

Results In RT-PCR analysis of the peripherial blood, 45%(18 out 40) of CRC patients, 10%(2 out 20) of CRP patients were positive for CEA mRNA, respectively. All 40 healthy individuals were negative for CEA mRNA expression. The detection of CEA mRNA was significantly correlated with TNM stage of CRC patients, while there were no significant relationship between the CEA mRNA expression and the malignant extent of cell differentiation.

结果 40例结肠癌患者外周血中CEA mRNA表达的阳性率为45.0%(18/40)，而健康对照组均为阴性，两者比较有显著性差异(P.01)；20例结肠息肉患者中CEA mRNA表达阳性率为10%(2/20)；CEA mRNA阳性表达率与肿瘤分期相关，而与肿瘤细胞分化程度并不相关。

Results The rejection group began to show the mRNA of perforin and granzyme B up-regulation on day 3 posttransplantation, when no histologic evidence of rejection was found. Levels of expression significantly increased after day 4 posttransplantation and persisted through the whole course of rejection, but levels of expression were obviously lower in the peripherial blood than those in portal vein blood. In contrast, the control group showed no expression of mRNA for perforin and granzyme B in portal vein and peripherial blood.

结果 急性排斥肝移植组术后3天门静脉和外周血中均有穿孔素和颗粒酶B基因mRNA表达，4天后表达明显增强，并贯穿整个排斥反应的全过程，但外周血表达的强度明显较门静脉血为弱，同基因对照组术后14天内无穿孔素和颗粒酶B基因表达。

The newly discovered endogenous vasodilating and diuretic peptide adrenomedullinwas considered to be of attractive value in clinical treatment of hypertension and congestive heart failure.

为探索通过体内表达肾上腺髓质素(adrenomedullin， AM)治疗高血压和慢性心衰的可能性，本实验构建了重组AM真核表达载体，并在无内源性AM表达的凡62细胞株上进行了体外表达实验。

Methods Using the long-distance RT-PCR and a mammalian expression system, the full length cDNA of the α1D was cloned from cochleae and then expressed in HEK293 cell line.

以耳蜗基底膜中组织总RNA为模板，利用长距离RT-PCR克隆耳蜗毛细胞表达的电压门控钙通道α1D剪切异构体，并利用异源表达系统在哺乳动物细胞系进行表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。