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Functionally known genes were obtained after Blastx analysis, 237 of them were from the subtractive library of stage of proembryo mass and 279 from that of somatic embryo maturation.

成功构建了Y35体细胞胚原胚团阶段和成熟阶段的差异表达基因文库,获得了868个差异表达的UniESTs,其中原胚团阶段400个,体细胞胚成熟阶段468个。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术,构建了斜带石斑鱼生长激素(Growth hormone,GH)基因重组质粒,在大肠杆菌细胞中获得高效表达,亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原,免疫家兔制备特异性抗体,通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交/核糖核酸酶保护试验及化学发光检测技术,研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制,对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Our study displayed that HmgD overexpression mutant died as late third instar larvae and malformed pupae during the larvae-prepupa transition,suggests that overexpression of HmgD has effects rather on the third instar larvae stage than on the first and second instar larvae from the mutant could not pupate.

结果表明,HmgD广泛过量表达突变体幼虫在一龄和二龄期发育正常,而在三龄幼虫化蛹时发育异常,发育停滞在三龄幼虫末期至预蛹的阶段,即HmgD过量表达的幼虫不能正常化蛹。

Objective: to investigate the mechanism about the effect of danshensu on the transforming growth factor beta 1 signaling pathway in Rattus norregicus hepatic stellate cells .

以肝星状细胞株HSC-T6为研究对象,免疫细胞化学法观察丹参素对TGFβRII表达的影响,RT-PCR法检测丹参素对Smad2和Smad4表达水平的影响,检测结果做图像分析处理。

Through seriate genome-wide mRNA expression data, similarity between two genes could be measured.

通过分析酵母细胞的大规模基因表达谱数据,获得不同基因间表达的相关性。

Excluding interference of breeding conditions' distinctness, this trial compared the difference of gene' expressing in liver which closely correlated with Silkie's, normal plumed black-bone chicken's, SuQin96's and Abar acer broiler's unique traits.

本试验分别以1日龄丝羽乌骨鸡、常羽乌骨鸡、苏禽蛋鸡、爱拔益加肉鸡肝脏为研究材料,期望在排除饲养条件等环境因素对基因表达的影响下,比较初生蛋鸡、肉鸡和乌骨鸡基因表达上的差异。

Naphthylamide heavy nitrogen spectrophotography was adopted to detect the content of nitric oxide in the serum and half quantitative reverse transcription polymerase chain reaction was used to detect eNOS gene expression in artery vessels.

采用萘胺重氮分光光度法测定血清NO含量,采用半定量RT PCR方法检测各组动物动脉壁匀浆中eNOS基因的表达,分析造模及各药物组血清NO含量及eNOS基因表达的变化。

The expression of 260 genes were detected in all stages of spermatogenic cells.

利用cDNA微阵列技术分析了1176个小鼠已知基因在不同阶段生精细胞以及成熟精子中的表达谱,发现了多个阶段差异表达的基因。

Similar results have been obtained using spermidine of 10ppm.6BA of 5 ppm promoted female tendency,but that of 10ppm promoted male flower.

1引言外源植物激素、生长延缓剂和有关物质对某些植物性别表达的影响已有报道[4_5_7_11、14、17],但关于青萍性别表达方面的研究极少。

Objective To study the COX-2 and PINCH Protein expression in oral squamous cell carcinoma and the relationship between them.

目的研究COX-2及PINCH蛋白在口腔鳞状细胞癌中的表达情况,探讨二者在口腔鳞状细胞癌中表达的相关性。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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