表达的

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子，我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异，发现ZF(HCF-binding transcription factor，Zhangfei)和SH3GLB1（SH3-domain GRB2-like endophilin B1）在这三种不同来源的红细胞内具有显著的表达差异，并通过实时定量PCR方法验证了差异的真实性。

Objective To investigate the effects of the Chinese drug Zilongjin and hexamethylene bisacetamid on expression of oncogenes(c-ras,c-myc),and tumorsuppressor genes(Rb,p53,p21)of MGc803 cell in cell cycle.

目的 探讨中药紫龙金与环六亚甲基二己酰胺对人胃癌MGc80 3不同周期细胞癌基因c ras、c myc、抑癌基因Rb、p5 3、p2 1以及蛋白激酶PKC a基因表达的影响。方法采用Northernblot印迹法检测基因表达情况。

Results:The positive rates of TSP- 1 mRNA in hypertrophic scars and keloid were lower than those in normal skin and surgical scar. MVC was higher in the cases with negative expression of TSP - 1 mRNA than the ones with positive expression of TSP - 1 mRNA. MVC was higher in the hyperstrophic scar and keloid than in normal skin and surgical scar.

结果TSP-1 mRNA在增生性瘢痕和瘢痕疙瘩中表达的阳性率低于在正常皮肤和外科瘢痕组织中的表达(P＜0.05)；TSP-1 mRNA表达阴性病例组的MVC显著高于TSP-1 mRNA阳性病例组的MCV(P＜0.05)；增生性瘢痕和瘢痕疙瘩组织中微血管数多于正常皮肤和外科瘢痕组织的微血管。

In o rder to explore the relationship bet ween HSP70and P-g p,the human hepatocarc inoma line HepG2cells was induced by heat shock in vitro,and the inhibiting effect of quercetin on them was obs erved at the same time to seek the method incre asing the effectiveness of hyperthermal therapy on hepatocarcinoma.

本研究拟通过热休克诱导人肝癌HepG2细胞表达HSP70和P-gp，并观察槲皮素(quercetin，Qu)对二者表达的抑制效应。

Thyroid hormone responsive spot 14, expressed in lipogenic tissues, is suggested as a transcription factor to regulate gene expression of rate-limiting enzymes in lipogenesis. Two THRSP isoforms, THRSPα and THRSP β, were detected at cDNA levels in chickens and ducks.

甲状腺激素应答Spot 14 (Thyroid hormone responsive spot14， THRSP)是一个参与多种脂肪合成限速酶基因表达的转录调控因子，在动物肝脏、乳腺和脂肪组织中高度表达。

Results:Comparing to the control group, the proliferation of overexpressed p16 QBC939 cells was inhibited and p16 overexpress induced G1 phase blockade.

结果：与对照组相比，p16高表达的人胆管癌细胞QBC939的增殖受到明显抑制；流式细胞光度术表明p16的高表达导致了较为明显的G1期阻滞作用，但对细胞周期的其他时相并无影响；Western免疫印迹分析结果表明癌基因c-myc的蛋白水平表达下降。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

To construct a expression vector induction by RU486 and confirm the regulatable effect in vitro.

构建可经RU486诱导表达载体，并证实其对基因表达的调控作用。

To construct a expression vector induction by RU486 and confirm the regulatable effect in v/tro.

构建可经RU486诱导表达载体，并证实其对基因表达的调控作用。

The ability to modulate the expression of multiple genes by tetracycline and its derivatives is likely to regulate the timing and level of gene expression, quantitatively induce the expression of toxic protein, increase therapeutic efficacy and safety, and provide a safe and regulatable way for gene therapy.

CHO-Tet-On细胞株的建立，可利用四环素及其衍生物调节多种外源基因的表达，有效调控基因表达的时间和水平，定量诱导毒性蛋白的表达，增加治疗的疗效和安全性，有望为基因治疗提供一条可控的安全途径。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。