表达的

In this article, the progress of baculovirus as a expression vector in mammalian cells was reviewed.

本文对杆状病毒作为一种表达载体在哺乳动物细胞中表达的研究进展进行了综述。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞，获得重组杆状病毒rBV-σC；SDS-PAGE和Western-blot分析表明，在sf-9细胞中表达了分子质量约37ku的σC蛋白，该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应，这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

Objective To investigate the significance of expression of p16 and bcl-2 in cervical intraepithelial neoplasia ang invisive cervical carcinoma.

目的探讨p16和bcl-2表达产物在宫颈上皮瘤样病变及宫颈癌中表达的意义。

However, there was a positive relationship between COX-2 gene expression and lymph node metastasis.

COX-2过表达可能通过对CyclinDl和Bcl-2表达的调控。

Methods:Phenobarbital and Valproic acid were added into astrocytes and cultured permanently for 30 days. P-glycoprotein, a product of MDR1, was detected with immunocyto chemistry. Then, 5μg/ml Sibelium was added to resistance cells and nonresistance cells, MTT assay was used to measure reverse multiple and relative reverse efficiency rate.

将苯巴比妥钠和丙戊酸钠分别加入培养的星形胶质细胞内，30天后用免疫细胞化学法检测MDR1的表达产物P-糖蛋白，继之将终浓度为5μg/ml的西比灵分别加入培养的正常细胞及MDR1表达增强的细胞内，用四甲基偶氮唑盐法计算逆转倍数和相对逆转效率，了解西比灵逆转MDR1表达的作用。

The recombinant protein was purified with the different pH. Western blotting testing of the porcine expression of pGEMX POB in E.coli BL21 was positive.

利用非融合表达产品制备抗血清，检测融合表达的重组蛋白，Western-blot为阳性。

Results RECK protein was expressed steadily in transfected HepG2 cells and the amount of activated MMP-9 were decreased significantly.

结果 成功构建了RECK基因真核表达载体并建立了稳定表达的细胞株。

The results suggest that EGFR overexpression, nm23-H1 low expression and negative correlation of their expression may be one of the causes of PTC?s biological feature of lymph node metastasis; serail tests of EGFR, nm23-H1 could be used as the markers to evaluate lymph node metastasis of PTC.

甲状腺乳头状腺癌EGFR的过表达，nm23-H1的低表达，二者表达的失平衡是其易于淋巴结转移的原因之一，EGFR，nm23-H1联用可作为甲状腺乳头状腺癌淋巴结转移的评价指标。

The CCND2 expression cell proliferation, cell cycle and cell apopotosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.

设计、合成CCND2短发夹RNA双链DNA模板，构建表达质粒pGenesil-2／CCND2，转染LP-1细胞，以RT—PCR检测对CCND2表达的抑制作用，以锥虫蓝染色计数和MTI检测细胞增殖，以Annexin V检测细胞凋亡，流式细胞术检测细胞周期。

Almost none of the non tumorous bladder tissues were positive for these genes.

肿瘤组织中6种基因至少有1种表达的频率是75.8%(25/33)，两种或两种以上同时表达几率是69.7%(23/33)。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。