表达的

And its developmental expression pattern showed that AmphiRab23b was expressed in the differentiating neural plate and alimentary canal, as the same as the expression pattern of the homologous vertebrate genes, which suggested that AmphiRab23b may function in the development of nervous system and alimentary canal.

时空表达的研究结果进一步显示，AmphiRab23b基因在胚胎发育中的神经板和消化道中表达，与其脊椎动物同源基因的表达模式相似，这说明该基因可能对文昌鱼神经系统和消化道的发育有重要作用。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的，尽管硫氧还蛋白有很强的助溶作用，该蛋白依然不可溶形式存在；NS5B—dc21蛋白通过C末端疏水序列的删除，获得了可溶表达；NS5AB蛋白以不可溶形式表达，需要复性。

Owing to the common expression of EGF in various organs of mouse,it could be deduced that the role of EGF was mainly autocrine and paracrine actions,and its endocrine role was also not excluded owing to its high expression abundance in submaxillary gland.

由于EGF在小鼠各器官中表达的普遍性，可以推测EGF的作用以旁分泌和自分泌作用为主，但它在颌下博士学位论文"TnEGFAlelittin"嵌合基因构建及有关基因重组表达的研究等器官中的高表达表明其也具有内分泌作用。

In this study, real-time PCR technology was used to dectect the expression of PRL mRNA and its receptor in the reproductive tissues,which is hypothalamus-pituitary-ovary-oviduct four important tissues. The aim was to compare the difference of PRL mRNA expression in the different state of ovulatory, broody and after broody, and to analyse the relation between expression and reproduction.

本试验利用real-time PCR技术检测雪山草鸡下丘脑、垂体、卵巢、输卵管四个繁殖轴组织中PRL基因及其受体的表达量，以确定处在生产、抱窝、醒抱三个不同状态母鸡PRL基因表达的差异，并分析基因表达量的差异与生产性能的相关性。

After 72 h induced by Progesterone and Glucocorticoid as well as Insuline,biological activities of IL-2 were assayed with morphological docimasia method.

结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3 OVP—IL2的输卵管上皮细胞表达产物在1：256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

After 72h induced by Progesterone and Glucocorticoid as well as Insuline, biological activities of IL-2 were assayed with morphological docimasia method. The results showed that the plasmids of pcDNA3-QVP-IL2 and pcDNA3-IL2 could express IL-2, and the IL-2 could augment T cell proliferation.

结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3-OVP-IL2的输卵管上皮细胞表达产物在1：256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

LX2 cells were transfected at the adenovirus tite pfu100, and were harvested at different times (24,48,72h), then western blotting was executed to detected the expression of ectogenous ampk.

对凋亡信号通路的进一步研究发现，Bcl-2在LX2中是低表达的，而Bax在AMPK过表达之后，表达增加，而用RNAi方法敲低Bax之后，细胞再用携带AMPK基因的腺病毒感染，LX2细胞不再发生凋亡，说明Bax参与了AMPK诱导LX2凋亡的信号通路。

The mice were used as experimental animals in this study,through the experiment in vitro and in vivo,systemic invested the dynamic changes in expression and localization of FGF7,FGF10 and its receptor in mammals development,lactation and involution;revealed the relationships between the expression of FGF7,FGF10 and its receptor and the function;clarified the function of FGF7 and FGF10 in mammary gland development,lactation and involution,and the effect of mammogenic hormones on expression of FGF7 and FGF-10 and its receptor in different periods in vitro.

本研究主要以小鼠为实验材料，通过体外和体内实验，系统研究FGF7、FGF10及KGFR在哺乳动物乳腺发育、泌乳及退化过程中表达定位的动态变化，揭示FGF7、FGF10及KGFR表达变化与乳腺发育及泌乳功能间的对应关系，阐明FGF7、FGF10在乳腺发育、泌乳及退化过程中的功能以及乳腺发育激素对不同时期乳腺FGF7、FGF10及KGFR表达的影响。

Our results suggest that several signaling pathways are involved in regulating Tbx6 expression, and pave the route to reveal the molecular mechanism of how myogenesis is initiated.

Xnot对Tbx6早期起始以及晚期维持表达都没有影响，提示其它基因参与限制Tbx6的空间特异性表达。我们的结果揭示了可能参与调控Tbx6表达的上游基因，为进一步揭示肌肉发生起始的机制提供了研究基础。

Methods: The human KDR promoter (-225 bp~+127 bp) was cloned by PCR. Subsequently, a recombinant adenoviral plasmid carrying KDR promoter for the HSV-tk gene was constructed with the AdEasy system. As a control, the plasmid pAdCMV-tk carrying CMV promoter for the HSV-tk gene was also constructed. 293 packaging cells were transfected by both newly-constructed plasmids and the infectious viruses AdKDR-tk and AdCMV-tk were generated. Then the KDR-producing cells and the KDR nonproducing cells (HepG2) were infected with AdKDR-tkand AdCMV-tk, followed by ganciclovir administration.

应用PCR克隆出人KDR基因启动子序列－225bp~+127bp，以AdEasy system为载体，构建携带受KDR启动子或CMV启动子调控tk基因表达的2种重组腺病毒质粒pAdKDR-tk和pAdCMV-tk，在293细胞中包装、扩增后，体外感染表达KDR的脐静脉血管内皮细胞系HUVEC和不表达KDR的肝癌细胞系HepG2，并给予不同浓度的丙氧鸟苷处理，5d后收集存活细胞并计数。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。