表达的

Gotoh Y, Nishida E, Yamashita et al. Microtubule associated protein kinase activated by nerve growth factor and epidermal growth factor in PC12 cells: identity with the mitogen-activated protein kinase of fibroblastic cells. Eur J Biochem, 1990, 349: 661～669.[7]Cook SJ, Beltman J, Cadwallader KA et al.

本文观察到，在AngⅡ介导的心肌细胞肥大反应中，伴随着MAPK的激活， MKP-1蛋白表达增加，若以AT1受体阻断剂CV11974或MAPK激酶特异性抑制剂PD098059抑制MAPK激活，同时也抑制了MKP-1蛋白表达，提示MAPK的激活可能是MKP-1蛋白表达的诱导因素之一。

Up-regulated proteins under salt stress include an ABC transporter permease, glycerol-3-phosphate permease, pyrimidine nucleotide transporter, formate dehydrogenase, and down-regulated proteins include succinate dehydrogenase iron-sulfur subunit, flavoprotein subunit, cytochrome b-556 subunit, and a hypothetical membrane protein similar to charperone DnaJ. Some of these changes were further verified by enzyme activity assay.

盐胁迫诱导上调表达的蛋白包括ABC型转运蛋白，3-磷酸甘油透性酶，嘧啶核苷转运蛋白和甲酸脱氢酶；下调表达的蛋白包括琥珀酸脱氢酶铁硫亚基、黄素蛋白亚基、细胞色素b556亚基，以及分子伴侣DnaJ的同源蛋白，酶活力测定结果表明胁迫条件下上述蛋白的活性变化与表达量变化相一致。

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子，本研究构建了带报告基因的载体并检测了其启动子，增强子和减弱子的功能，以此为基础，进一步构建了MBP异位表达载体，为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

Due to instantaneousness and interactivene ss of everyday communication , vague expressions in everyday conversation are quite different from those in written forms .

英语日常口语交流中存在大量的模糊表达现象，作为口语重要的一部分，其特点及功能因为口头表达的即时性与互动性与书面表达存在着很大的不同。

Wash my clothes very cleanly and I have plap ping Pong foot ball. The end I do myhomework and go to bed!

这样做虽然不及参考答案水平高，但降低了书面表达的难度，既能提高书面表达的信心，也能提高书面表达获得更高考分的可能性。

Sp were evaluated by computer-assisted image analysis software, and the content of OPG determined by immunohistochemistry, which included mean optical density 、positive area density in bone trabecula and total positive area of cancellous bone, were evaluated by computer-assisted image analysis and semiquantitative analysis.

Sp，免疫组化方法结合图像分析半定量分析OPG在胫骨的表达，测定平均光密度、OPG在骨小梁中阳性表达的面积密度和OPG在松质骨骨组织阳性表达的总面积。

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

Using functional clustering and statistic methods, three gene clusters and 47 genes highly expressed in Landrace heart tissue were found.

通过比较分析长白猪4种组织的基因表达，找出了长白猪心脏组织中3个显著高表达的功能群和47个显著高表达的基因。

The functionof seed specific promoter Napin cloned by our lab was confirmed in Arabidopsis, it can drivegene expression in the seed derived cotyledons and hypocotyl in seedling stage, and in theskins of siliques in the mature stage.

在拟南芥中检验了本实验室克隆的油菜种子特异表达的启动子 Napin，表明 Napin 驱动基因在幼苗的子叶和下胚轴表达，在成熟期驱动基因在角果皮中表达。

The expressions of thedifferentially expressed genes (gamma-A-crystallin and neural tropomodulin) werefurther analyzed in the retinae of rds, rd and C3B mice using real-time quantitativeRT-PCR assay. Based on the sequence of ESTs from the differential display, thefull-length genes were amplified using the RACE method.

实验方法：以生后第12天、第25天和第37天为研究时段，应用mRNA差异显示与cDNA表达阵列等方法分析rds小鼠发病过程中视网膜差别表达的基因，然后用Northern blotting和实时荧光定量PCR等方法，分析差别基因在rds小鼠和rd小鼠视网膜的表达，所分析的时间为小鼠整个生命周期。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。