表达的

AIM: To detect the differentially expressed genes in the process of esophageal carcinoma induced by nitrosamine, and analyze the property of gene expression at different stages, especially the relationship between differentially expressed genes and DNA methylation.

摘要目的：通过基因芯片技术检测亚硝胺诱导食管癌变过程中，食管组织基因表达的差异，分析不同阶段基因表达的特点以及基因差异表达与DNA甲基化的相关性。

Ore-binding factor alpha 1 plays an essential role in bone formation and osteoblast differentiation and serves as a master gene regulating osteoblast-specific gene expression. The gene is expressed in cells of the osteoblast lineage only, and when expressed in non-skeletal cells, the cells assume many of the characteristics of an osteoblast.

bfal在骨形成和成骨细胞分化过程中起着重要的作用，是调控成骨细胞特异性基因表达的主控基因，该基因只在骨形成相关细胞中表达，如果使其在非成骨细胞中表达，该细胞将出现成骨细胞的特征。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

Among these 35 peaks, 21 of them expressed much more strongly in triploid abalone while 14 of them expressed more strong in diploid one.

二维液相色谱共获得35个差异表达的蛋白质组分，3n中表达量显著高的21个、2n中表达量高的14个。

The p53 gene structural abnormality and mdm2 protein overexpression might be two factors causing p53 protein overexpresion in NPC. The function of p53 proteins produced by various structural anormality of p53 gene might be different in activating MDM2. The overexpression of p53 or mdm2 protein, particularly both cooperation, might play an important role during NPC genesis.Key words: Nasopharyngeal neoplasms; p53 gene; p53 protein; mdm2 protein

p53基因结构改变和mdm2蛋白过度表达是影响NPCp53蛋白过度表达的两个因素；不同类型p53基因结构改变所产生的p53蛋白激活MDM2的功能并不相同；p53和mdm2蛋白过度表达，尤其两蛋白协同作用，可能在NPC发生中起重要作用。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

The analysis of 99 Tcm-MIBI scinti-photo graphy may be a sensitive index in detecting P-gp expression in vitro.

(1)99Tc-MIBI转运分析可以作为体外检测P-gp表达的敏感指标，P-gp高水平表达的乳腺肿瘤组织其晚期相摄取比值及滞留指数明显低于无P-gp表达的肿瘤。

Results The expression plasmid of pVAX1 IL-18 HN was contructed successfully confirmed by enzyme digestion identification. The expressions of IL-18 and HN chimeric genes were confirmed, and the expressed HN protein had higher hemagglutinative titer.

结果：酶切鉴定证实正确地构建了pVAX1 IL-18 HN嵌合表达质粒，Western blotting和血凝试验检测结果表明，嵌合后的IL-18和HN基因均能够表达，且表达的HN蛋白具有较高的血凝活性。

There is Cx43 expression in the human thyroid epithelial cells, and there are heterogenicity in levels of Cx43 expression in autoimmune thyroid diseases.

Cx43可在人甲状腺上皮细胞中表达；Cx43在AITD的表达强度存在异质性，这种缝隙连接蛋白表达的异质性可能与AITD的发生、发展及预后有关。

Though the molecular mass and the hypoglycemic activity of myristic acid modified HIDesB30 are both consistent with Detemir, the expression system, purification artwork and the methods of modification were different from Detemir.

本研究实现了含短C肽的人胰岛素原类似物HMPIDesB30在毕赤酵母中的高效分泌表达，表达量达1 g/L，分泌表达的重组蛋白HMPIDesB30未出现糖基化，未形成二聚体和多聚体，并具有生物学活性。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了