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The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体,以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板,参照已发表的微小牛蜱Bm86基因的核苷酸序列,设计了1对引物,采用RT-PCR技术获得微小牛蜱Bm86基因;将Bm86基因克隆入载体,并进行序列分析,结果证明,克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%,而且该序列包含完整的开放阅读框。

In this paper, the myogenic bHLH gene, AmphiMDF, expressed in the embryos of amphioxus (Branchiostoma belcheri tsingtaunese), has been cloned by the methods of degenerative PCR and RACE.

本文利用简并性PCR和RACE等方法克隆了青岛文昌鱼(B.belcheritsingtaunese)胚胎发育过程中表达的生肌bHLH基因,AmphiMDF。

In bronchi and bronchioli smooth muscles.(5) There were no difference between protein expression of these two potassium channels when examined in whole lung tissue.

慢性吸烟下调大鼠气道平滑肌BKca和Kv1.5的表达水平,可能是导致气道高反应性的机制之一。

Objective To investigate the protein and gene expression in bronchoalveolar lavage cells and open lung biopsies from patients with IPF.

目的 观察血小板衍生的生长因子AA、BB及转化生长因子(TGFβ在特发性肺间质纤维化支气管灌洗细胞及开胸肺活检标本的蛋白及基因表达,探索其在IPF发病中的作用。

Objective: To investigate the expression of Th1 chemokine CXCL11/I-TAC and its receptor CXCR3 in the lesions of bullous pemphigoid.

目的: 研究Th1趋化因子CXCL11/I-TAC及其受体CXCR3在大疱性类天疱疮皮损中的表达。

The burring and randomicity of assessment is fully expressed in this method.

此方法可充分表达评价语言的模糊性和随机性,具有较大的客观性。

Objective To investigate the expression of PCNA, EGF and VEGF in gastric mucosa of patients with CAG and their clinical value.

目的 观察慢性胃炎胃黏膜上皮细胞增殖细胞核抗原、表皮生长因子及血管内皮生长因子的表达特点,提高慢性萎缩性胃炎的诊治水平。

Calcified rheumatic valve neoangiogenesis is associated with vascular endothelial growth factor expression and osteoblast-like bone formation.

钙化的风湿性瓣膜的血管新生与血管内皮生长因子的表达和成骨样细胞骨形成相关

Methods PTTG and VEGF were examined in 41 cases of primary gallbladder cancer and 20 cases of chronic calculous cholecystitis tissues with immunohistochemical assay.

应用免疫组织化学法,检测41例原发性胆囊癌及20例慢性结石性胆囊炎中PTTG和VEGF的表达水平。

PTTG and bFGF were examined in 41 cases of primary gallbladder cancer and 20 cases of chronic calculous cholecystitis tissues by immunohistochemical assay.

应用免疫组织化学,检测 4 1例原发性胆囊癌及 2 0例慢性结石性胆囊炎中PTTG和bFGF的表达水平。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。