表达性

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Iral expression of a mutant Rac1 decreased astrocyte motility and increased the length of dendritic filopodia but not their motility or lifetimes.

变异Rac1的病毒性表达降低星形胶质细胞的活动性，增长树突丝状伪足的长度但不增加其活动性和寿命。

Objective To understand the distribution and immune activation status of T lymphocyte subsets in highly exposed HIV1 and persistently seronegative individuals, and explore the immunologic mechanism of nonsusceptibility to HIV1 by sexual transmission in HEPS individuals.

目的 了解经性传播途径高暴露HIV1持续血清阴性者淋巴细胞亚群表达及其免疫活化状态，探讨经性途径高暴露HIV1不易感的免疫基础。

It showed that genes of self-incompatibility expresses during special term;③compatibility of selfing generation of variety with compatibility tend to decline.

具有自交不亲和性的品种，其自交不亲和性表现在特定的时期，说明控制自交不亲和的基因只在特定时期表达。

VR1 selectively expressed on small-to medium-diameter DRG neurons and it was recently considered as a molecular integrating multiple pain-producing stimuli.

VR1特异地表达在初级伤害性感受器上，被认为是汇集伤害性刺激的一个信号转导分子。

And then, we propose a Reincarnate Tree Model based on backup/restore mechanism that efficiently manage and improve the recoverability and well-operated of backup data.

针对此类产品中备份索引表达力弱，忽视备份数据间内在联系而导致备份数据管理GUI视图的友好性差、误操作高，恢复代理自主性弱等问题，提出了一种基于备份恢复机制的再生树模型以高效管理备份数据、提高备份数据的可恢复性与易用性。

Both of OsBIMK2 and OsBISERK1 genes were rapidly activated during 6h after BTH induction and maintained higher levels of tobacco brown spot pathogen Alternaria alternata.

转基因烟草T〓代植株中PR-1基因表现为组成型增强表达。抗病性分析表明，转基因烟草植株提高了对番茄花叶病毒ToMV和赤星病菌的抗病性。

The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Co. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin in liver tissues of all mice.

采用60Co治疗仪γ射线对实验组和对照组行350cGy的亚致死剂量照射，实验组A、B、C3组照射后3h内经尾静脉分别输入1.0×10^7个/只、2.0×10^7个/只和3.0×l0^7个/只人新鲜脐血有核细胞，对照组经尾静脉注入等体积无菌生理盐水。1个月后对实验A、B组和对照组裸鼠经腹膜腔注射四氯化碳(CCl4)，实验C组注射等体积生理盐水。2个月后采用免疫组化和RT-PCR方法检测裸鼠肝组织人源性CK18、CK19和人源性白蛋白的表达。

On the one hand, the paper theoretically develops the relations between online public opinion and public decision-making as well as its background connotation of rights and powers and their complicated linkages, based on which the"should-be"state of the relations between the foregoing two is proposed, on the other, it also analyzes, in the light of the states quo, the"is-being"state of the contradictions between the two sides.

一是从理论上梳理网络民意和公共决策的关系，****其背后的权利和权力意蕴和复杂纠葛，提出网络民意和公共决策关系的&应然&状态；二是从现实出发，对两者间的矛盾的&实然&情形进行分析，具体考察了网民结构、网络自由离散特征、网络情绪化表达等问题同公共决策民主、科学、理性要求的冲突，以及公共决策中对待网络民意的消极态度、&自利&倾向和技术方法上的被动和偏差等问题同网络民意的正当性、利益性、自主性要求之间的矛盾，并提出对各问题的反向思考和意见。

Wave distress Dose lives in the B.C.E. 3 centuries, is precisely Greece melts custom sculpture development the time, touches nearly to the life various aspects, from the unusual refined divine nature, starts to express the most universal human nature.

波厄多斯生活在公元前3世纪，正是希腊化风俗性雕塑发展的时代，几乎触及到生活的各方面，从超凡脱俗的神性，开始表达最普遍的人性。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。