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The results indicate that the presence of GSH increased the expression of exoY、exoS、phzA1 and gacA,while no difference was observed with others virulence factor genes tested.PAO1 produced markedly less pyocyanin than the wild type strain PAOIP<0.01Treating with GSH, the PCN production of PAOI(△gshB was increased from 0.47μg/mL to 1.73μg/mL (P<0.01),the PCN production of PAO1 increased from 1.3μg/mL to 1.9μg/mL(P<0.05),this result indicates that GSH upregulates PCN production.

结果发现,GSH对启动子库中exoS、exoY、phzA1和gacA等致病性因子的表达有促进作用,对其余测试过的致病性因子则没有明显影响;铜绿假单胞菌分泌的重要致病因子绿脓菌素(pyocyanin,PCN)的含量,在PAO1中远低于PAO1P<0.01加入GSH后,PCN平均含量在PAO1(△gshB中从0.47μg/mL提高到1.73μg/mL,差异十分显著(P<0.01),在PAO1中从1.3μg/mL提高到1.9μg/mL,差异显著(P<0.05),表明GSH对铜绿假单胞菌生产PCN有促进作用。

With the development of gene engineering technology,It becomes an efficient way to improve the cold resistance of plant that directly increase the cold resistance of plant by transferring anti-cold revelant genes into plant and make them express.

随着基因工程技术的发展,利用导入抗寒相关基因并使其表达,依靠其产物直接提高植物抗寒性,成为目前有效改善植物抗寒性的重要途径。

Methods of governing with media are as follows: releasing information in time, expressing to public the attitude and standpoint of the government, declaring revolutional policies, setting agenda , planning press briefing and so on.

媒介执政的方式有:及时发布最新消息、公开表达政府的态度和立场、传达具有时新性和变革性的政策、采取有影响的行动、设置一段时期内的主要议题和策划新闻发布会等。

He experiment results indicate that (1) The extract of Rubia Cordifolia L has the effects of anti-proliferation and inducing APO;(2) Whenadministrating with 5-fu, the extract of Rubia Cordifolia L obviously enhanced the effects of anti-proliferation and inducing APO of 5-fu;(3) The basement of molecular biology of the extract of Rubia Cordifolia L for inducing APO of MGC-803 cells is correlated with inhibiting the expression of bcl-2 of anti-apoptosis gene.

UNEL法与PCNA的免疫细胞化学法的结果:0.2、0.4、0.8、1.0mg/ml浓度的茜草提取物与2、4、8、16μg/ml浓度的5-Fu,分别诱导细胞凋亡和抑制细胞增殖,表现出浓度、时间依赖性关系,除个别浓度与个别时间之间无显著性差异(P>0.05)外,不同浓度与不同作用时间之间均有显著性差异(P<0.05或P<0.01),并且两种药物联合使用时,MGC-803细胞的APO%显著提高,降低PCNA的阳性表达率(P<0.05或P<0.01)。

Objective: To detect the expression of VEGF in human cerebral saccular aneurysm and explore the pathogenesis of aneurysm.

目的:检测VEGF在破裂人脑囊性动脉瘤中的表达,探讨脑囊性动脉瘤的发病和破裂机制。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体,病毒载体和非病毒载体以前已广泛应用,病毒载体主要包括腺病毒,逆转录病毒,腺相关病毒等;但病毒载体在安全性方面存在潜在的危险;免疫原性比较强,注射到机体后很快会被机体的免疫系统排斥,当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应;非病毒载体目前常用的有脂质体及多聚阳离子聚合物;但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性,转染效率较低且易被网状内皮系统吞噬,基因表达时间短;因此研制新型的非病毒载体已成为研究的热点,纳米颗粒具有小尺寸效应,表面效应,随着颗粒直径变小,比表面积将会显著增大,故具有很高的化学活性,因而纳米成为了最有应用前景的非病毒载体。

Based on a cognitive analysis of the generation of voice, Croft argues that passives are functionally generated by expressing a causative event into a stative event.

Croft从认知的角度对语态经过考察得出结论:被动态产生于把过程性的事件表达成状态性的事件这一过程。

The degrees of fibrosis of sternomastoid muscle in CMT are related to the hyperplasy of collagen type IV.

病变组中纤维型组IV型胶原较肌肉型组有更多的表达,两者比较有显著性差异(P.01),纤维型和肌肉型组与对照组比较均有显著性差异(P.01)。

Objective To investigate the expression and hyperplasy of Collagen Type Ⅰand Ⅲ in sternomastoid muscle of congenital muscular torticollis and to explore the pathogenesis of sternomastoid muscle fibrosis.

目的研究先天性肌性斜颈胸锁乳突肌Ⅰ、Ⅲ型胶原的增生和表达情况,探讨先天性肌性斜颈胸锁乳突肌纤维化的发生机制。

Apoptosis may be also involved in the pathogenesis of neuronal death induced by this thrombotic ischemic lesion.

血栓性脑缺血损伤中存在细胞凋亡现象及凋亡相关基因Bc1-2、Bax的表达,细胞凋亡也参与了血栓性脑缺血所致的神经细胞死亡过程。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。