表达性

The PEA gene product was detected in the medium with Western blot, which indicated that the PEA gene with original signal peptide could be expressed with secretary form in eucaryotic cells.

表达产物经蛋白免疫印迹法（Western-blot）检测，表明本文构建的含有原基因信号肽的PEA基因能够在真核细胞进行分泌性表达，这为基于PEA的免疫毒素、疫苗佐剂和疫苗载体的研究奠定了基础。

The transcription of hsp70 was detected in the major tissues of hepatopancreas, muscles, eyestalks under both normal and heat shocked conditions .The mRNA level was increased after the animals were heat shocked in the 3 tissues.

因此本文所克隆的中国对虾hsp70是一种无明显组织特异性的组成性表达的基因，其转录表达能被某些应激进一步诱导，使转录水平在本底基础上提高。

Hypercellularity and increased deposition of IgG in glomeruli were also observed.

三、结论 1.HAX-1在活动期SLE患者PBMC中的表达明显高于正常对照者，表达水平和SLE的病情活动性呈正相关。

It′s death rate locates the top among gynecological malignant tumors.Mucins is a family of high-glycosylated and high-molecular weight glycoprotein,which is distributed on the surface of body epithelial cells.In cancer cells,the expression of mucins is obviously high and their structures and functions are changed.Mucins are directly implicated in the infiltration and metastasis of ovarian tumors.

黏蛋白是一类高度糖基化、相对分子质量高的糖蛋白，分布于人上皮细胞表面，在卵巢上皮性肿瘤中表达水平明显上调，出现异常表达，其结构和功能发生改变，直接参与了肿瘤的浸润和转移，因而与卵巢肿瘤的发生、发展及预后相关。

Other dimensions of father's involvement were not variated in terms of children' age. Father's involvement was not different in terms of children's gender. Fathers who were androgyny were involved more than other fathers of sex role types and who was masculinity involved more than femininity and undifferentiation.

双性化的父亲对儿童的互动监督、规则约束、情感表达、间接支持、学业鼓励均显著多于其他性别角色的父亲；男性化的父亲对儿童的规则约束显著多于女性化的父亲；女性化的父亲在互动监督、情感表达、间接支持和学业鼓励方面均显著多于未分化的父亲。

Other dimensions of father's involvement were not variated in terms of children'sage. Father's involvement was not different in terms of children's gender. Fathers who were androgyny were involved more than other fathers of sex role types and who was masculinity involved more than femininity and undifferentiation.

双性化的父亲对儿童的互动监督、规则约束、情感表达、间接支持、学业鼓励均显著多于其他性别角色的父亲；男性化的父亲对儿童的规则约束显著多于女性化的父亲；女性化的父亲在互动监督、情感表达、间接支持和学业鼓励方面均显著多于未分化的父亲。

In order to obtains the medical gout vaccine through using the genetic engineering method, there is need to obtain the most superior expression choice to the uricase gene in which kind of animal model appropriate to express the object.

为利用基因工程手段获得治疗性痛风疫苗，需要对尿酸酶基因在何种动物模型中得到最优表达选择合适的表达对象。

Immunohistochemical staning for VEGF-C,VEGFR-3 and CD 31 was performed in epithelial ovarian carcinomas, borderline tumors and benign cystadenomas.

应用免疫组织化学法，检测卵巢上皮癌、交界性肿瘤和良性肿瘤组织 VEGF－C mRNA和蛋白表达、VEGFR－3及 CD 31的表达。

In contrast, deletion of the propeptide yields mature but inactive NK, the inclusion bodies of NK or denatured rNK couldn't be renatured in any condition, but with the aid of exogenously purified propeptide or Ser221Ala proNK, the inactive mature protein could be renatured by an intermolecular pathway in vitro.

此外，我们利用融合基因表达系统在大肠杆菌中高效表达了谷胱甘肽S-转移酶／NK和硫氧还蛋白／NK两种融合蛋白，经体外复性、纯化，均获得活性蛋白。以上结果显示，pro肽在体内体外，通过分子内或分子间的方式均能帮助NK分子的折叠与再折叠，作为一种分子内的分子伴侣，pro肽为NK分子正确折叠所必需。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。