表达性

Sixty six male Sprague-Dawley rats were randomly divided into control group,and 5 model hepatic fibrosis groups perfused alcoholic,maize oil and pyrazole for 2 weeks,4 weeks,8 weeks, 12 weeks and 16 weeks (11 rats in each group) respect...

结果 与对照组比较，酒精灌胃 4周、8周、12周、16周组大鼠OPN、α SMA表达显著增强并且肝内胶原纤维含量显著增加。正常肝组织中几乎不表达OPN ，在酒精性肝纤维化大鼠模型的建立过程中，从第四周开始中央静脉。。。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

(1)raising of PCNA protein expression after pre-operative radiotherapy of esophageal carcinoma may signify the repopulation of tumor cells.(2) The prognosis differed from different PCNA expression before or after radiation, it may be beneficial to select the best treatment plan and as the evaluating criteria.

(1)食管癌放疗后PCNA蛋白表达升高，可能存在加速再增殖；(2)疗前、疗后PCNA蛋白表达的预测性不同，可指导临床选择最有效治疗方案，可作为评价预后指标。

The expression product was analyzed and its antigenicity was further studied by ELISA and Wstern blot method with chimeric protein as coating antigen.we used chimeric protein as coating antigen to test anti HBs with ELISA and Wstern blot in 58 seral samples.

通过ELISA和Western blot检测表达产物的抗原性，并以表达产物为包被抗原，通过ELISA和Western blot检测58份乙肝患者血清抗 HBs。

There were significant difference with the frequency and strongness of PCNA in breast benign disease and breast carcinoma.

在良性病变与癌组之间，PCNA阳性表达率及表达程度差异有显著性(P＜0.05)。

Results: The positive unit of survivin expression intensity in trophocyte cytoplasm and karyon was subsequently increased in following order: normal early gestation villi, hydatidiform mole, invasive hydatidiform mole and choriocarcinoma.

结果：survivin在滋养细胞细胞核及细胞浆的表达强度PU值依下列顺序增加：正常早孕组、葡萄胎组、侵蚀性葡萄胎组及绒癌组，各组间的表达强度差异有统计学意义(P.05)。

A pure and single K+ current of ultrarapid delayed rectifier K+ current or transient outware K+ current is respectively expressed on Xenopus Oocytes. This is a moderm pharmacological model for evaluation class Ⅲ antiarrhythmiv durgs, overcomes many defcts such as lacking human fresh cardiac muscle cells as material and co-expression of many currents on cell membrane. A new compound A1998 from Ocean material is evaluated in this model for class Ⅲ antiarrhythmic functions.

5和Kv4.2cDNA转录为mRNA，然后将其分别注入蟾蜍卵母细胞上：在蟾蜍卵母细胞上分别获得纯净、单一的超速延迟性整流钾电流(Ikur，ultrarapid delayed rectifier K+current)和瞬间外向钾电流(Ito，transient outward K+current)表达，克服以往在筛选评价抗心律失常药物时，人新鲜心肌细胞取材困难、多种电流在细胞膜表面共同表达等缺点，从而建立评价Ⅲ类抗心律失常药物的先进药理模型，并筛选具有Ⅲ类抗心律失常药理作用的新化合物A1998。

The distribution of those abnormal expressions mentioned above tended to be extensive and homogenous in unruptured aneurysms, while localized in ruptured ones.

以上异常表达在囊性动脉瘤壁中的炎性反应区较为明显。在破裂动脉瘤的瘤壁中趋于局部集中表达，而在未破裂动脉瘤的瘤壁中分布较为均匀。

The intrapulmonary arteries especially arterioles are more important for vasoconstricting response to hypoxia , but it is very difficulty to isolate them from the lung.

我们以往通过对常压缺氧大鼠模型的肺组织和肺动脉肾上腺素受体(Adrenoceptor，AR)亚型及其基因表达的一系列研究，初步认为AR亚型基因表达变化在低氧性肺动脉高压发病中可能起重要作用。

However, higher concentration of CRP may increase the expression of protein and mRNA of inflammatory factor in endothelial cells; 2 Higher concentration of CRP may participate in the development of inflammatory process in endothelium by the mechanism that CRP induce cells apoptosis and activate signal pathway involved in cell proliferation. 3 CRP is the member of pentraxins family, in which two faces of pentamer form a special bi-phase structure: one face is ligand recognition phase; the other face is effector phase.

总结： 1CRP是一种保守蛋白，在正常生理水平下对内皮细胞没有明显的诱导炎症发生的作用，仅在高浓度条件下有可能诱导细胞炎症相关蛋白及mRNA的表达； 2高于生理水平的CRP对内皮细胞的致炎作用主要通过诱导细胞凋亡及激活细胞增殖相关蛋白信号通路的表达； 3CRP是一种双面五聚体蛋白，结构具有特殊的二相性，能够分别结合内皮细胞和E-LDL。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。