表达性

Objective:To investigate the expression of Tim-3 mRNA in murine corneas during recurrent herpetic stromal keratitis, and its relationship with HSK.

目的：通过检测小鼠单纯疱疹性角膜基质炎复发过程中Tim-3 mRNA在角膜组织的表达水平，探讨Tim-3的表达与HSK复发之间的关系。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71％and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54％and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52％,and the activity of penicillin G acylasewas in creased by about 76％;about 90％of Hexameric Calcitonin(Cal6)inclusion body and l5％of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中，周质空间分泌蛋白总量较对照组提高了约71％，GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍，碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54％；在过量表达GroEL的宿主菌中，周质分泌蛋白总量较对照组提高了约52％，青霉素G酰化在周质空间酶的活力较对照组提高了约76％，鲑鱼降钙素六聚体的可落性组分的比例由原来的45％增加到约90％，而MS2-人白介素-3融合蛋白的包涵体有约15％转变为可溶性组份。

It was found by means of protein fluorescence detection that the number of MAP-2 masculine neurone in the hippocamp of AD rat in model group and zinc reinforce post-AD group decreased distinctly. There was not distinct difference in the MAP-2 expression and the number of MAP-2 masculine neurone between normal group and zinc reinforce pro-AD group.

模型组和AD后补锌组大鼠海马区MAP-2蛋白表达与正常组大鼠相比显著下降，MAP-2蛋白荧光检测发现模型组和AD后补锌组大鼠海马区MAP-2阳性神经元数量明显减少，AD前补锌组大鼠海马区MAP-2蛋白表达和MAP-2阳性神经元数量与正常组相比差异无显著性。

Results compared with the normal villi,the quantity of P21 expression has no significant difference,the site of P21 expression in the hydatidiform mole is quite different.

结果 与正常绒毛相比，P21癌基因在葡萄胎组织中的表达量没有显著性差异，表达部位有明显不同。

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

Objective: The first aim of this study was to investigate bcr-abl associated T cell receptorVαand Vβgene repertoire by analyzing the restricted usage and clonal expansion of TCRVαand Vβsubfamily T cells from the normal individual peripheral blood induced bybcr-abl peptideso The second aim was to construct the idiotype TCR Vα-PIRES-TCR Vβeukaryotic vector and investigate its expression in vitro. The data will be expected toprovided the methods in development of the leukemia antigen specific cytotoxic Tlymphocyte modified by the specific TCR.

目的：通过分析bcr-abl多肽诱导正常人T细胞克隆性增殖和TCR Vα和TCR Vβ基因选用情况，获得识别bcr-abl多肽的特异性TCR基因，并构建TCR Vα和TCR Vβ双基因表达载体及分析其体外转染细胞和表达特点，从而为研制TCR转基因获得白血病抗原特异T细胞的研究提供实验依据。

After transfection of E.coli BL21 and induction by IPTG,the recombinant immunotoxin fusion protein was highly expressed in E.coli BL-21 in the form of inclusion bodies(up to 32.29% of total cellular proteins).

IPTG诱导后目的蛋白获得高效表达，SDS-PAGE分析目的蛋白主要以不溶性包涵体的形式存在于菌体中，表达量占菌体总蛋白的32.29％。

Methods Immunohistolchemical staining was used to detect the expression of PTEN in 50 cases of primary breast infiltrative ductal carcinoma tissues.

采用免疫组化技术检测50例原发性乳腺浸润性导管癌中PTEN的表达情况。结果：PTEN总的表达缺失率为44％(22／50)。

Expresion of FasL was found mainly in the cytoplasm of liver-infiltrating mononuclear cells,these positive cellswere found particularly at the advancing edges of piecemeal necrosis,bridge necrosis and in the intralobular.

以免疫组化法研究Fas配体在丙型肝炎病人肝组织内的表达情况，发现在碎屑样坏死、桥样坏死及灶性坏死区浸润的单个核细胞有明显的FasL表达，阳性率为41.2％。

It can intuitionally describe the hiberarchy and parallelity of algorithms. In modeling, DDF can express various control structure, such as conditionals and data dependent iterations.

DDF是一种简单、完备而且表达能力强的并行计算模型，能直观地表达算法的层次性和并行性。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。