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METHODS: The total RNA was obtained by TRIzol from an antiricin neutralizing monoclonal antibody hybridoma cell named 4C13 and heavychain and lightchain variable fragments were amplified by overlap PCR and accepted by Genbank of NCBI. VH and VL were linked by3 with a direction of VHlinkerVL and cloned to pCDNA3.1 vector expression system.

用TRIzol提取抗ricin中和性单抗杂交瘤细胞4C13总RNA,扩增其轻、重链可变区基因,登陆GenBank;用Linker3将VH和VL连接,用重叠延伸PCR克隆入真核表达载体pCDNA 3.1,用脂质体转染293T细胞,对瞬时表达产物进行生物学活性检测。

Here, we presented a method to bacterially express the major structural protein L1 of Human Papillomavirus type 18 (HPV18) as soluble form. We found that the purified L1 could self-assemble to virus-like particles.

利用大肠杆菌表达系统可溶性表达人乳头瘤病毒18型(HPV18)L1蛋白,经过纯化和重组装过程获得HPV18病毒样颗粒,研究其免疫原性和诱发中和抗体生成的水平。

The high prevalence of resistance to chicken sera, fimbriae F1, temperature-sensitive hemagglutinin in high pathogenic isolates suggested importance of these phenotypes in the pathogenesis of colibacillosis.

结果显示禽源大肠杆菌的致病性与其血清耐受能力、鸡红细胞MSHA菌手的表达和温度敏感性血凝素的表达呈一定的相关关系。

That is to say, when we are weighing the contemporariness of a work, only the seeming "expression" is never enough, we have to further examine the effectiveness of its "expression"--is the question new, is the perspective unique and the reflection profound.

也就是说,我们在衡量艺术品的当代性时,不是看似有&表达&就可以了,还要进一步追问作品&表达&的有效性--其问题是否新颖、视角是否独特、思考是否深刻。

However, the level of NGF protein in the contusive and lacerated brain tissue was statistically lower in the aged group than in the young group (P.05). Conclusion The expression of NGF after TBI is influenced by age.

结果 脑损伤后NGF在老年和青壮年脑挫裂伤组织中的表达均明显增强;老年组脑挫裂伤组织中的NGF蛋白表达显著性低于青壮年组(P <0.05)。

ConclusionThe expression of α-B crystallin in IDC is significantly higher than that in DCIS and is positively related to lymph node metastasis. There are also higher levels of expression in the HER2+ and the triple-negative groups. This suggests that α-B crystallin is involved in the process of progression and metastasis of breast cancer.

结论α-B crystallin在乳腺浸润性导管癌中的表达明显高于导管原位癌,与淋巴结转移呈正相关,且在生物学行为更为恶性的HER2+组和三阴性组具有更高的表达水平,提示α-B crystallin蛋白可能参与了乳腺癌的浸润与转移过程。

For many years, she has been completely involved in the artistic state of the uncontrolled daubing and spontaneous rendering, in a mode of extremely sensitive feeling and bold experimentation to narrate her own emotions abstractly, just like in a fairy tale wonderland full of pleasure, unrestricted imagination and unlimited creativity.

许多年来,她完全沉醉于任意涂抹和随性表达的艺术状态之中,以极为敏锐的感性和大胆的实验,用抽象的形式叙述自己的情感,就像处在一个童话世界里,充满着表达的快乐、无拘无束的想象和充沛的创造力。

By means of names, designations, and deictic expressions, we refer to individual objects, whereas sentences in which such singular terms occupy the subject position usually express a proposition or report a state of affairs.

借助于名字,称号和指示性表达,我们指称摸个对象,这些东西在句子当中的占据了主要的位置,他们经常表达一种主题或者一种事态。

The active form of NGF is a dimer in which two monomer is linked by 3 disulphide bonds. This form of dimer makes the purification of NGF difficulty because the protein expressed in E.

在蛋白纯化过程中这种由二硫键形成的空间结构往往是蛋白纯化过程中复性的难点,尤其是在大肠杆菌表达生物蛋白时,所表达的蛋白往往以无活性的单体形式存在于细胞内。

The expression rate of GST-π in grade 1 and grade 3 and in domical stage Ⅰ-Ⅱ an...

GST-π的过度表达可能与卵巢上皮性肿瘤的发生有关,对于卵巢恶性肿瘤的早期诊断具有一定意义,且GST-π的表达与临床分期及组织学分型均无明显相关性。

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推荐网络例句

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。