表达性

Recombinant mouse enterokinase can be expressed in Ecoli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

In addition, the r8 cDNA was also expressed at high level in pBAD/TOPO system and showed esterase activity after enterokinase cleavage followed by purification and renaturation.

此外类凝血酶基因r8在pBAD／TOPO中也得到高效融合表达，表达产物经酶切、纯化和复性，测得精氨酸酯酶活性。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

Results Compared with CIA models,100 mg·kg~(-1)·d~(-1) TMP remarkably reduce the arthritis index,footpad thickness,microvess...

结果100 mg.kg-1.d-1川芎嗪组的大鼠关节炎指数和足垫厚度均低于生理盐水组(P.05)；滑膜微血管密度降低，VEGFmRNA表达及VEGF蛋白表达也明显减少，与模型组相比差异均有显著性(P.05)。

In order to explore the clinical prospect of Peimine, we select acute leukemia patients as objects, and investigate the followings:(1) Relationship between P170′s expression and its clinical effect in AL;(2) Mechanism of Thurberg Fritillary Bulb and Peimine in reversing MDR in AL;(3) Combined with chemotherapeutic drugs, the ability of Thurberg Fritillary Bulb to decrease P170's high expression and its clinical efficacy in AL management;(4) Relationship between Traditional Chinese syndrome and P170′s expression or clinical efficacy.

为了探讨浙贝母的临床应用前景，考虑到取材的方便性，我们选择了急性白血病为研究对象，做了以下几方面的研究工作：(1)P170在急性白血病中的表达及其与临床疗效的关系；(2)浙贝母散剂和贝母甲素逆转急性白血病细胞多药耐药的机制探讨；(3)浙贝母散剂临床合用化疗时对急性白血病细胞多药耐药蛋白P170的逆转作用及其临床疗效；(4)中医证型与P170表达及临床耐药关系的研究。

Results: A different expression cDNA subtractive bank was successfully established from the human nasopharyngeal carcinoma cell line CNE2Z; Six cDNA clones were highly expressed in the lower metastatic strain CNE2ZH5; aligned to sequences of the GenBank database by BLASTN, the six cDNA fragments were found to homologize highly with known human genes.

结果：成功构建人鼻咽癌细胞克隆株差异表达cDNA消减文库，从随机测序10个克隆中获得6个在低转移鼻咽癌细胞株中高表达cDNA序列，它们都与已知的人类基因片段具有高度同源性。

The level of the expression was correlated with clinicopathological factors. Results MRP1 was mainly expressed on carcinoma cell membrane and cytoplasm, and in the positive expression cell, the membrane and cytoplasm was huffy.

结果 MRP1蛋白主要是在细胞膜和细胞浆内表达，MRP1蛋白在大肠癌原发灶组、正常组织组的阳性表达率分别为49.1%(26/53)和15.1%(8/53)，两者有显著性差异(P.01)。

Aim To construct anti-HFRS virus human engineering antibody expression vectors and then to express it in eukaryotic cells.

目的构建人源性抗HFRS病毒基因工程抗体基因的表达载体，为其进一步在真核细胞中表达奠定基础。

The results showed that P51 was expressed as inclusion body, and represented about 50% of total cellular protein. After purification and renaturation, the purity of P51 was up to 95%.

结果显示P51以包涵体的形式表达，表达量占菌体总蛋白的50％，经两步层析和透析复性，目的蛋白纯度大于95％。

Tumour biopsies from melanoma patients obtained after treatment show the presence of intracellularly localized nanoparticles in amounts that correlate with dose levels of the nanoparticles administered (this is, to our knowledge, a first for systemically delivered nanoparticles of any kind).

为了验证这个传导系统和siRNA是否对癌细胞有专嗜性，科学家们又取了正常组织，结果发现，瘤组织中的RRM2信使RNA表达量和RRM2蛋白表达量均比正常组织的低，这表明siRNA正在发挥RNAi的作用。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。