表达性

Beginning with the speech act theory, the intention and effect of speech act verbs are explored, and the corresponding classifications are put forward. From a further analysis of how to express"attempt"and"success"in conative functional semantic field, the expression means of how to express"success"in Chinese and Russian speech act verbs are induced and the differences of the two core expression means are pointed out by a comparative study.

本章从言语行为理论出发剖析言语行为动词的意向性和取效性，据此对其进行分类，分析了意动功能语义场下的言语行为动词如何表达"尝试"义和"成功"义，推导出汉、俄语言语行为动词对"成功"义的表达手段，通过对比分析指出两种语言核心表达手段的差异。

To solve this problem, we consider that use of tissue-specific regulative elements in construction of plasmid vector for molecular conjugate vector may limit the expression of foreign gene to targeting cells further.

为解决这一问题，我们考虑在构建分子偶联载体中的重组质粒表达载体时，应用组织细胞特异性调控序列，以期在转录水平进一步限制外源基因的表达于靶细胞中，从而保证受体介导基因转移的靶向性，同时可望导致外源基因的高效表达。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

The identity and difference in the expressional patterns between distribution functions of discrete random variables and distribution functions of continuous random variables are elaborated,and the main expressional patterns of distribution functions of continuous random variables are summarized,and the derivability of distribution functions of continuous random variables is discussed.

0引言在概率论中，连续型随机变量分布函数的表达形式及其可导性是一难点。其一，离散型随机变量的分布函数有较明显的性质和统一的表达形式，而连续型随机变量，由于其概率密度函数一般为分段函数，根据其分段区间的差异，表达形式差异较大，不容易总结其规律，本文主要比较连续型随机

The recombinant mouse enterokinase light chain could be well expressed in Ecoli, but most of them were inclusion.

利用pET32a/BL21(DE3)表达系统成功地在大肠杆菌中表达了重组鼠源性肠激酶轻链，表达量约占大肠杆菌BL21(DE3)总蛋白的30%，但多以包涵体的形式存在。

Up-regulated proteins under salt stress include an ABC transporter permease, glycerol-3-phosphate permease, pyrimidine nucleotide transporter, formate dehydrogenase, and down-regulated proteins include succinate dehydrogenase iron-sulfur subunit, flavoprotein subunit, cytochrome b-556 subunit, and a hypothetical membrane protein similar to charperone DnaJ. Some of these changes were further verified by enzyme activity assay.

盐胁迫诱导上调表达的蛋白包括ABC型转运蛋白，3-磷酸甘油透性酶，嘧啶核苷转运蛋白和甲酸脱氢酶；下调表达的蛋白包括琥珀酸脱氢酶铁硫亚基、黄素蛋白亚基、细胞色素b556亚基，以及分子伴侣DnaJ的同源蛋白，酶活力测定结果表明胁迫条件下上述蛋白的活性变化与表达量变化相一致。

Due to instantaneousness and interactivene ss of everyday communication , vague expressions in everyday conversation are quite different from those in written forms .

英语日常口语交流中存在大量的模糊表达现象，作为口语重要的一部分，其特点及功能因为口头表达的即时性与互动性与书面表达存在着很大的不同。

Methods The differentially expressed genes between liver tumor and non-tumor tissue were cloned by using suppression subtractive hybridization. Differentially expressed genes were sequenced and analized for homology in Gene Bank. In situ hybridization was performed to identify special expression of differentially expressed genes in livre tumor and non-tumor tissues.

方法通过抑制消减杂交法克隆土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因；对差异表达基因进行DNA序列测定后于Gene Bank内分析其同源性；应用原位杂交确定差异基因在肝癌组织和非癌组织中的特异性表达。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的，尽管硫氧还蛋白有很强的助溶作用，该蛋白依然不可溶形式存在；NS5B—dc21蛋白通过C末端疏水序列的删除，获得了可溶表达；NS5AB蛋白以不可溶形式表达，需要复性。

The cloning, sequencing, homological blasting and Northern blotting results of 5 desiccation-induced cDNAs and 3 phosphate induced cDNAs implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as G protein, protein kinase, VP3and MAD3-like genes might be involved in dehydration in the resurrection plant Boea hygrometrica.

对其中5个脱水特异诱导表达的cDNA（包括可能与复苏能力有关的cDNA）和3个磷酸盐处理诱导表达的cDNA进行克隆测序、同源性探测和Northern杂交检测表明，牛耳草脱水过程中诱导表达的基因可能涉及到脱水胁迫的信号转导、调节基因的级联作用（VP3，MAD3样基因等）、结构基因产物调节细胞结构在脱水胁迫中的稳定性等。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。