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To analyze WWP1 protein expression in clinical samples, we applied IHC technology to detect the level of WWP1 protein in 18 paired OSCC samples, our data represented different they had different protein pattern and location.

应用免疫组织染色法侦测18对成对口腔癌样本WWP1蛋白表现,发现不同的样本呈现不同的蛋白表现量,表现在细胞的位置也有所不同。

But they were partial sterile, had 1~4 pollen sacs in each anther and had relative bigger petals and stamens at lower temperature situation. The RGCMS plants were complete sterile at the both temperature situations, and there was no pollen sac differentiation and had smaller peta...

表现核质不育的植株,在高温条件下,花瓣小,表现彻底的polCMS不育,无花粉囊的分化;在低温条件下,花瓣变大,也表现彻底雄性不育,具有核、质不育的共同特点,每个花药可分化出 1~ 4个花粉囊,但无可育花粉,其雄性败育时期和特点与S45A和 117A相同。

The DGCMS plants were complete sterile at the both temperature situations, and there was no pollen sac differentiation and had smaller petals and stamens at the higher temperature situation, but there were 1~4 pollen sacs in each anther and had bigger petals and stamens at the lower temperature situation. The cytological characteristics of the anther development were the same as that of GMS plants in Yi-3AB.

表现质核不育的植株,在高温条件下,花瓣小,表现彻底的pol CMS不育,无花粉囊的分化,在低温条件下,花瓣变大,也表现彻底雄性不育,具有核、质不育的共同特点,每个花药可分化出1~4个花粉囊,但无可育花粉,其雄性败育时期和特点与宜3A相同。

They were partial sterile, had 1~3 pollen sacs in each anther and had relative bigger petals and stamens at lower temperature situation. The DGCMS plants were complete sterile at the both temperature situations, and there was no pollen sac differentiation and had smaller petals and stamens ...

表现质核不育的植株,在高温条件下,花瓣小,表现彻底的 pol CMS不育,无花粉囊的分化;在低温条件下,花瓣变大,也表现彻底雄性不育,具有核、质不育的共同特点,每个花药可分化出 1~ 4个花粉囊,但无可育花粉,其雄性败育时期和特点与宜 3A相同

Morphological and cytological observations showed that the pol CMS plants in DGCMS-Yi-3A were complete male sterile, had no pollen sac differentiation and had smaller petals and stamens at higher temperature situation. They were partial sterile, had 1~3 pollen sacs in each anther and had relative bigger petals and stamens at lower temperature situation.

花器形态和花药发育的解剖学观察结果表明,DGCMS-宜3A中表现波里马细胞质雄性不育的植株,其花器形态和花药发育的解剖学特点与pol CMS相同,即在高温条件下,花瓣较小,表现彻底不育,无花粉囊的分化,在低温条件下,花瓣变大,表现部分雄性不育,可分化出1~4个花粉囊,并产生一定数量可育花粉。

Morphological and cytological observations showed that the pol CMS plants in DGCMS Yi 3A were complete male sterile, had no pollen sac differentiation and had smaller petals and stamens at higher temperature situation.

花器形态和花药发育的解剖学观察结果表明,DGCMS-宜 3A中表现波里马细胞质雄性不育的植株,其花器形态和花药发育的解剖学特点与 pol CMS相同,即在高温条件下,花瓣较小,表现彻底不育,无花粉囊的分化;在低温条件下,花瓣变大,表现部分雄性不育,可分化出 1~ 4个花粉囊,并产生一定数量可育花粉。

Morphological and anatomical observationsshowed that the pol CMS plants in DGCMS-Yi-3A were complete sterile and hadno pollen sac differention at higher temperature condition,and were partialsterile and had 1-3 pollen sacs in each anther at lower temperature condition.

花器形态和花药发育的解剖观察表明,DGCMS-宜3A中表现波里马细胞质雄性不育的植株在高温条件下表现为彻底雄性不育,无花粉囊的分化;在低温条件下,表现部分雄性不育,可分化出1-3个花粉囊,产生少量可育花粉。

Morphological and anatomical observations showed that the pol CMS plants in RGCMS?117A or RGCMS S45A were complete male sterile ,had no pollen sac differentiation and had smaller petals and stamens at higher temperature situation.

花器形态和花药发育的解剖学观察表明,RGCMS S45A和RGCMS 117A中表现波里马细胞质雄性不育的植株,其花器形态和花药发育的解剖学特点与polCMS相同,即在高温条件下,花瓣较小,表现彻底不育,无花粉囊的分化;在低温条件下,花瓣变大,表现部分雄性不育,可分化出 1~ 4个花粉囊,并产生一定数量可育花粉。

The RGCMSplants in RGCMS-117A or S45A were complete male sterile at the bothtemperature conditions,and there was no pollen sac differentiation at thehigher temperature condition and there were 1-3 pollen sacs in each anther atthe lower temperature condition,but the anther development was inhibited atthe stage of microspore.

表现核质不育的植株,在高温条件下表现彻底波里马细胞质雄性不育,无花粉囊分化;在低温条件下,也表现彻底雄性不育,具核、质不育的共同特点,每个花药可分化出1-3个花粉囊,但无可育花粉,其雄性败育时期和特点与S45A和117A相同。

Results:(1)Among 40 rats, 36 rats were successfully established and the rate of success is 90 percent;(2)All the successfully established models demonstrated polydipsia, polyuria and the body weight was not increased. 6 rats suffered cataract after 3 months, and 4 rats died in 6 months;(3)There was an approximately 61% loss of retinal ganglion cells in the central retina and the thickness of retina thinner under microscope ( P 0.01 ).(4) Electroscope changes include the thinner of retina, disorganization of the membranous disc of the rod cells and the thickness of basal membrane of vessal.(5)In normal group, 1 month dibetes mellitus and 1 month treatmen group, there was no expression of ERK1/ERK2 on the retina tissues. In 3 month diabetes mellitus group, the expression of ERK1/ERK2 was positive.

结果:①40只建模大鼠中36只建模成功,建模成功率为90%;②建模成功的大鼠都表现出多饮、多尿、消瘦、体重不增的表现,有6只大鼠在3个月后出现白内障,有4只大鼠在喂养接近至6个月时死亡;③HE染色光镜下6个月大鼠后极部视网膜节细胞层细胞数明显减少,减少约61%(P.01),后极部视网膜明显变薄(P.01);④电镜观察,视网膜变薄,视杆细胞膜盘紊乱,血管基底膜增厚等表现;⑤正常组、糖尿病组和治疗组1个月大鼠视网膜中未见ERK1/ERK2的表达,糖尿病组视网膜组织中3个月时可见少量表达,ERK1/ERK2表达部位为神经节细胞层和内核层;6个月时表达强阳性,部位表达不仅见于内核层、神经节细胞层,色素上皮层也见表达。

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