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Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰,直径约1mm,成斑时间12h;从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号: AY639599),又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因,表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli, E.coli)中表达获得了47kDa的清晰表达带,在1h 时开始产生蛋白并有逐步上升的趋势; Western blot 也在47kDa处得到一条清晰的条带;可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的,该蛋白的产生明显地抑制了宿主的生长速度;噬菌体PEP氨基酸序列之间的同源性比较表明,噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

This paper analyses the different concentrations of Pb, Zn ion hybrid seedlings on the soil culture camphor several physiological indicators of the impact of the results show that the experimental results show that, POD, SOD, CAT, such as the biological oxidation resistance of the show is basically change the trend promoting high-low suppression trend, proline, MDA membranaceous permeability, as well as the content, with the concentration of heavy metals increases, reflecting the biofilm was heavy metal ions damage situation.

本文分析了不同浓度的Pb、Zn混合离子对土培香樟幼苗的几种生理指标的影响,结果表明,实验的结果表明,POD,SOD,CAT等抗氧化性的生物酶的改变趋势基本上呈现低促高抑的趋势,而脯氨酸,丙二醛以及膜质透性的含量是随着重金属浓度的增大而增大,体现出了生物膜被重金属离子的破坏的情况。

This paper analyses the different concentrations of Pb, Zn ion hybrid seedlings on the soil culture camphor several physiological indicators of the impact of the results show that the experimental results show that, POD, SOD, CAT, such as the biological oxidation resistance of the show is basically change the trend promoting high-low suppression trend, proline, MDA membranaceous permeability, as well as the content, with the concentration of heavy metals increases, reflecting the biofilm was heavy metal ions damage situation.

求高手翻译一段话,好的话多给100分!本文分析了不同浓度的Pb、Zn混合离子对土培香樟幼苗的几种生理指标的影响,结果表明,实验的结果表明,POD,SOD,CAT等抗氧化性的生物酶的改变趋势基本上呈现低促高抑的趋势,而脯氨酸,丙二醛以及膜质透性的含量是随着重金属浓度的增大而增大,体现出了生物膜被重金属离子的破坏的情况。

No entire nitrendipine crystals were observed visually in SEM photos. The results of X-ray diffraction and differential scanning calorimetry analysis indicated that the crystalline form of nitrendipine was highly dispersed in microspheres, so as amorphous state. The drug release rate of microspheres could be controlled with adjusting the ratio of solid dispersion carriers and retarding agents formulated. The agitation speed and temperature of the preparation process have distinct effect on micromeritic properties of microspheres. The release profiles of the microspheres were mainly affected by the stirring rate of paddle, the concentration of SDS and pH of dissolution medium. Cooling speed and time, however, have no evident influence on the drug release rate of the microspheres. The dissolution data showed that the mechanism of drug release from microsphers was mainly diffusion-controlled. The incorporation efficiencies of 3 batches sample were exceed 96. 8%, which implied that the current method was suitable for design sustained-release dosage form for poorly water-soluble drug.

在扫描电子显微镜下观察,在微球内外未发现尼群地平的完整结晶,X-射线粉末衍射和差示扫描量热试验结果也显示,尼群地平已经被高度分散在微球中;微球的释药速度可通过调整处方中固体分散体载体和阻滞剂的比例控制;制备温度和搅拌速度对微球的质量影响较大;溶出仪的搅拌速度,释放介质的浓度和pH对微球的释放有较大的影响;制备过程中的冷却速度和冷却时间对微球的释放行为影响不很明显;方程拟合的结果表明缓释微球的释药行为符合扩散机制;测定三批微球样品的包封率均在96.8%以上,表明该法适合于制备难溶性药物的缓释微球。

Kalamaili suture zone is an important plate boundary in north Xinjiang, and there are plenty of Neopaleozoic volcanic rocks around itThese volcanic rocks are enriched in LILEs and relatively depleted in HFSEs, and they are also characterized by high Nb, Zr, TiO2 contents and Zr/Y, Nb/Y ratios, and depleted SrNd isotopesThe characteristics of these volcanic rocks suggest that they were produced in postcollisional periodThe postcollisional volcanic rocks around Kalamaili suture zone become younger from west to east, which indicates that this suture zone entered postcollisional period gradually from west to east, and reflects that the Kalamaili Ocean had been closed gradually from west to eastAs to the Kalamaili suture zone in postcollisional period, the lithosphere extended and thinned, and the asthenosphere materials upwelled and melted partially, and intensive mantle convection occurred, leading to a plenty of mantlederived magmas underplating the suture zoneThe lithospheric mantle metasomatized by fluid in subduction period melted partially under decompression and high temperature, the resulting melts contaminated the underplating asthenospheric materials, and the erupted lavas became the Neopaleozoic postcollisional volcanic rocks

卡拉麦里缝合带是北疆的一条重要界线,沿该带广泛发育晚古生代火山岩。这些火山岩具有LILE相对富集、HFSE相对亏损的特征,且具有较高的Nb、Zr、TiO2含量和Zr/Y、Nb/Y比值,SrNd同位素主要表现出亏损的特征。综合分析表明,这些火山岩形成于后碰撞期。对卡拉麦里缝合带而言,从西向东后碰撞火山岩的时代逐渐变新,表明该缝合带从西向东逐渐演化进入后碰撞期,这从侧面反映了卡拉麦里洋盆是从西向东逐渐关闭的。对卡拉麦里缝合带而言,在后碰撞期,岩石圈伸展减薄,软流圈地幔物质上涌并发生部分熔融,加上地幔对流作用强烈,使得缝合带下产生了大规模的幔源岩浆底垫;先前在洋壳消减期被流体交代提取过的岩石圈地幔也在低压高温下发生部分熔融,形成的熔体混染了底垫的软流圈物质并喷发,形成了晚古生代的后碰撞期火山岩。

Excitation functions of neutron, proton and α particle emission prior to scission measured in the reactions 28 Si+ 164,167,170 Er→ 192,195,198 Pb are studied using an extensive fission diffusion model and it is found that the model can well describe the trend of particle multiplicities of Pb isotopes measured experimentally as a function of the bombarding energy,which indicates the existence of isotope effects in prescission particle emission.

用推广的裂变扩散模型研究了2 8Si+16 4,16 7,170 Er→192 ,195,198Pb反应中测量的断前中子、质子和α粒子多重性的激发函数。发现扩散模型能很好地拟合这些轻粒子多重性随裂变系统激发能和Pb同位素的变化趋势。表明断前粒子发射存在一个同位素效应。进而在一个考虑了裂变过程中摩擦效应的统计模型的框架内,在In和Er这两个元素的同位素链中,考察了同位素效应与裂变碎片质量不对称性和裂变系统尺度的关系。计算结果表明:同位素效应的存在不依赖于裂变系统的大小和不对称性。此外,当裂变系统具有高的中质比时,轻带电粒子发射将不再敏感于裂变延迟时间的长短,而中子发射则非常敏感。

It showed that the niche breadth and niche overlapping indexes of the enclosed grassland in 2007 are much lower than in 2006 as a whole; The niche breadth of Artemisia ordosica is bigger than other species in the region in 2002. In 2003, the dominant species were Artemisia ordosica and Salsola ruthenica. Their niche breadth indexes were 0.544 and 0.460 respectively. The results of niche overlapping in 2006 and 2007 also showed that the species with big niche breadth didn't always have big niche overlapping, and the niche breadth and niche overlapping did not have a positive direct linear relation.

结果表明:2007年封育区植物种的生态位宽度及种间的生态位重叠指数总体上较2006年有所降低;2006年黑沙蒿生态位宽度最大,达0.768,是06年封育区的优势种;2007年封育区的优势种为刺沙蓬和黑沙蒿,其生态位宽度值分别为0.544、0.460;2006年和2007的生态位重叠结果均表明,生态位宽度大的物种不一定和其他物种有大的重叠指数,较高的生态位宽度和较高的生态位重叠之间并不存在直接的线性关系;2006年、2007年较高的Pianka生态位重叠指数都出现在生态位宽度较小的物种之间。

The analyses of social and economic development indicate that, up to 1998, the total population in Yijinholo County is 143, 949 persons, the population density is 24 persons per square kilometer, large population number results in land exploitation for food and overgrazing in rangeland. The change in temporal and spatial pattern of landuse from 1977 to 1998 demonstrates that mobile sand dunes was replaced by rangeland and woodland and partial shrubland in the middle of study area changed into rangeland and farmland from 1977 to 1987, while mobile sand dunes and rangeland transformed into shrubland and farmland in a small parts from 1987 to 1998, the above-mentioned change is proved by the change of fractal dimension index and fragmentation index at landscape and its element levels.

对近50年来伊旗社会经济发展的分析结果表明,虽然人口增长率基本上呈下降趋势,但由于人口基数较大,截至1998年底,伊金霍洛旗的人口总数达143949人,人口密度为24人/平方公里,较高的人口密度带来了更多的生存需求,使得大量的草牧场被开垦,而剩余的则严重超载。1977-1998年间伊土地利用结构总体格局变化的分析结果表明,1977-1987时段内主要是流动沙地为草牧场和乔木林地所取代,伴随着中部的部分灌木林地转换为草牧场和农地:而1987-1998时段内主要是草牧场和流沙地为灌木林地和农地特别是灌木林地所取代,而景观及各景观要素的分维度指数和破碎度指数的变化也充分证实了这一结论,虽然在前10年(1977-1987年)中大面积的灌木林被砍伐,但土地利用格局的整体变化间接地反映出该时段内荒漠化的状况正在发生逆转。

Then, according to phase space reconstruct technique from one-dimensional and multi-dimensional time series, the quantitative criterion and rule of system chaos which combine neural network, analyses, computations and sort are conducted on EEG signals of five kinds of human consciousness activities. The author finds that statistic results of the determinism computation in time series indicate that chaos characteristic may lie in human consciousness activities, and central tendency measure is consistent with phase graph, so it may become one division way of attractor; approximate entropy of different subjects exists discrepancy; correlation dimension and Lyapunov exponent of different consciousness activities indicate that attractors of human activities are all fractional; nonlinear quantitative criterion and rule, which unites neural network, can distinguish different styles of consciousness activity in principle, and the result of sortation takes on that consciousness activity type of arithmetic is distinguished more easily than that of abstract.

采用一维与多维时间序列相空间重构技术和系统混沌的定量判据准则,结合神经网络,对五种思维作业方式的脑电信号进行了分析、计算与分类,发现确定性计算的统计结果表明人类的意识活动中可能蕴含混沌特性,中心趋向测量(Central Tendency Measure,CTM)与相图吻合较好,可作为EEG吸引子的区分方法之一;功率谱分析反映出单个受试者的各种意识形态的差异很小,但是不同意识活动谱中的活跃频段还是略有差异的;个体之间的近似熵存在差异,同种状态下近似熵大的人可能具有更好的创新性;关联维数和Lyapunov指数的计算结果表明大脑的运动落在具有分维的奇怪吸引子上;非线性量化方法与神经网络相结合,可对人脑思维活动进行较好的分类,并且从分类结果来看数理计算类思维活动较抽象类思维活动有着更好的区分度。

The effects of solution\'s pH value, mol ratio between phenyl aldehyde and L-Arginine, concentrations of sodium chloride, ammonia chloride andL-Lystine, initial concentration of L-Arginine on precipitation rate of a -tolylene arginine were investigated, the results indicated that phenyl aldehyde precipitation method\'s appropriate pH value was higher than 11, suitable mol ratio between phenyl aldehyde and L-Arginine was 1.25, initial concentration of L-Arginine was must higher than 15g/L, sodium chloride had little effect on precipitation rate, on the contrary, ammonia chloride and L-Lystine had great effect on it, both of them must be eliminated; At the same time, adsorption isotherm at 25 C of L-Lystine on anion exchange resin A was measured, the result showed that the maximal equilibrium adsorbance was 30mg/g, the influences of solution\'s pH value, temperature, concentration of chloride ion on adsorption of L-Lystine by anion exchange resin A were also studied, the results indicated that appropriate operational pH value was 0.5 0.2, the effect of temperature on adsorption ratio was little, process could be operated at room temperature, the concentration of chloride ion must be eliminated.

考察了溶液pH值、苯甲醛与L-精氨酸摩尔比、氯化钠与氯化铵浓度、L-赖氨酸浓度、L-精氨酸初始浓度对苯甲醛沉淀L-精氨酸的影响,结果表明,沉淀反应的适宜pH值大于11,苯甲醛与L-精氨酸的适宜摩尔比为1.25,(来源:Aa6BC论文网www.abclunwen.com)L-精氨酸适宜的初始浓度在15g/L以上,氯化钠的存在对沉淀率基本上没有影响,而氯化铵和L-赖氨酸的存在使沉淀率下降;同时测定了25℃时阴离子交换树脂A吸附L-赖氨酸的吸附等温线,表明其最大平衡吸附量为30mg/g,考察了溶液pH值、温度、氯离子浓度对阴离子交换树脂A吸附脱除L-赖氨酸的影响,结果显示从L-精氨酸和L-赖氨酸混合液中吸附分离出L-赖氨酸的适宜pH值为10.5±0.2,温度对L-赖氨酸吸附率影响不大,吸附过程可在室温下进行,氯离子的存在使L-赖氨酸的吸附率降低。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。