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On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Analysis indicates that the adjacent channel power is a quadratic function of predistorter efficients. A new optimization method is given which uses the predicted ACP surface to obtain fast convergence and simulation results show that a 20dB reduction in the third IMD and a 10dB reduction in the quatic IMD are achievable.

得出结论为:分析表明功放输出的邻信道功率是预失真器系数的二次函数;利用了新的自适应算法即使用预测的ACP曲面以提供更快的收敛速度;仿真结果表明,3阶产物可获得20dB以上的改善,5阶产物可获得10dB以上的改善。

Preliminary research shows that supplemental vitamin A improves the likelihood that the measles vaccine will provide protection. 6 Vitamin A has, since the 1920s, been the subject of much research into the prevention and treatment of childhood exanthems, particularly measles. 7 This nutrient has a critical role in proper immune function , and there is evidence that supplementation with vitamin A reduces the incidence and severity of, and deaths from, childhood measles. 8 9 The World Health Organization has therefore recommended that children with signs of deficiency receive supplementation with vitamin A. The recommended amounts are 100,000 IU for children younger than one year and 200,000 IU for children older than one year, immediately upon diagnosis, and repeated once the next day and once in one to four weeks.

11初步研究表明,补充维生素A的可能性,提高麻疹疫苗将提供保护。6维生素A已自20世纪20年代,被纳入预防和童年exanthems,特别是麻疹治疗中的许多研究课题。7本养分有在适当的关键作用免疫功能,并有证据表明,补充维生素A减少发病率和严重程度,以及,儿童麻疹死亡。8 9世界卫生组织已因此,建议与不足的迹象儿童得到补充与维生素A的建议金额10万国际单位的儿童未满一年,20万国际单位的儿童一年以上,立即诊断,重复一遍,第二天,一次在1至4周。

Online tuning and relatively light calculation burden due to directly coefficient setting by formula extend this scheme to be used for fast-running process and mechanical equipment, numerical simulation over a wide range of system parameter variation shows that the recommended scheme is superior to both traditional PDF designed by Phelan's approach and relay feedback auto-tuning PID in terms of performance behavior .

大量的数值仿真试验表明,该自整定PDF控制方法具有良好的控制效果和在线调节性能,具有设计公式简单、相对计算量小的特点,能够应用于快速变化的过程和机械设备中。本文通过基于继电反馈的自整定PDF控制和基于Phelan法的PDF控制及基于继电反馈的自整定PID控制的比较研究,表明所提出的控制方法具有良好的控制效果。

Based on the crossing data among these flatfishes, the relationship between pairwise genetic distance and hybrid fitness was evaluated. The findings indicated that positive relationship might exist between genetic distance and hybrid fitness. The pairwise genetic divergence between species decreased as the interspecific hybrid fitness largened. These results implied that speciation clock might exsit in Pleuronectiformes.

根据这几种鲆鲽鱼的杂交是否可行的试验结果,评价种间遗传距离与杂交可适度的关系,结果表明,这8种鲆鲽鱼类的种间遗传距离与杂交可适度呈显著的负相关(r2 = 0.805,P 0.01),即种间遗传分化越大,杂交成功的可能性越小,这表明鲆鲽鱼类中可能存在物种进化的不亲和钟。

Golden-crowned kinglets tolerated colder temperatures in helox than ruby-crowned kinglets, and this difference was not a function of mass or thermal conductance, as these did not vary significantly between species. Msum in golden-crowned kinglets (2.51 ml±0.32 ml O2min-1, n=11) was significantly greater than that in both male (2.27 ml±0.25 ml O2min-1, n=13) and female (2.05 ml±0.18 ml O2min-1, n=13) ruby-crowned kinglets, suggesting that metabolic differences may account for differences in cold tolerance.

结果显示:金冠戴菊对低温的耐受能力高于红玉冠戴菊,由于金冠戴菊和红玉冠戴菊的体重和热传导差异不显著,表明对低温的耐受能力的差异不是体重和热传导引起的;而金冠戴菊的最大代谢产热(2.51 ml±0.32 ml O2min-1, n=11)明显高于雄性(2.27 ml±0.25 ml O2min-1, n=13)和雌性(2.05 ml±0.18 ml O2min-1, n=13)的红玉冠戴菊,表明最大代谢的差异可能导致对寒冷的耐受能力不同。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明,除了能复性成天然态的溶菌酶分子外,还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体;而它们的还原电泳和在SDS存在时的复性结果表明,这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成,而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

The washing experiment of soil contaminated by petroleum alkane was performed with two kinds of biosurfactants in laboratory. The results showed that biosurfactant would enhance on removal of residual alkane in soil, after washing with 20 pore volume solution the removal rate with BS-UC and MELs were three times and twice as that of water, respectively.

在实验室内对南极假丝酵母产生的两种生物表面活性剂进行了石油烃类污染土壤的冲洗实验,结果表明,经过大约20个孔隙体积的冲洗,BS-UC溶液的除油率为清水的三倍,MEL溶液为清水的两倍,这表明该生物表面活性剂能够加速土壤残油的去除,而来自污染底物的生物表面活性剂更具有优越性。

The morphology of virions seems to not be change after incubated with the paeonol by electron microscope. The regained infectivity of TMV recovered by dialysis from incubation with paeonol in vitro showed that the paeonol have not direct viricidal effect. The paeonol did not reduce infectivity when applied before TMV inoculation. The paeonol in low concentration can restrain the polymerization of TMV coat protein in vitro. The paeonol did not reduced infectivity of TMV-RNA in vitro. It is proposed that the paeonol inhibited TMV by binding with TMV coat protein to interfere with recognition of a host receptor essential for infection or by interfering TMV-CP polymerization in plants. The anti-TMV activity of low molecular weight chemicals extracted from Polygonum cuspidatum were determined.

丹皮酚与TMV混合,电镜下观察对粒体形态没有影响;TMV与丹皮酚混和后透析可恢复侵染力,表明丹皮酚对病毒粒体并没有直接毒害作用;预先用丹皮酚喷施叶面不影响病毒的侵染,表明丹皮酚未干扰TMV对叶片侵染位点的识别;丹皮酚在低浓度时可阻碍TMV-CP的体外聚合,对TMV-RNA并无体外抑制侵染作用,据此推测丹皮酚对TMV的抑制作用可能主要通过其与TMV-CP结合影响病毒粒体对叶片表面侵染位点的识别,或影响体内TMV粒体的脱衣壳或TMV-CP亚基的体内聚合。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明:盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域;而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深,这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明:在盐酸胍溶液中,随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深,其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多,直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基,在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个,而随着其分子变性程度的进一步加深,反而减少至5个。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。