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For the protein side chain,the band at 1 368 cm-1 assigned to the "buried" tryptophan shifted to 1 365 cm-1 of the "exposed" and the relative intensity reduced 59%. The relative intensity of 635 cm-1 assigned to the gauche conformation of the C—S decrased 15% and the band at 725 cm-1 increased 58%.These indicate that the structure of the protein changed in the diabetic serum.The relative intensity at 1 449 cm-1 assigned to the lipids characteristic increased 58%.The relative intensity of the glucide characteristic at 1 331,1 099 and 740 cm-1 increased 35%,100% and 62%,respectively.

蛋白质侧链色氨酸吲哚环&埋藏式&的谱线1 368 cm-1位移到&暴露式&的1 365 cm-1,且相对强度减少了59%;侧链C—S键的扭曲构象的谱线635 cm-1相对强度减少了15%,而反式构象的谱线725 cm-1增加了58%,说明C—S键的扭曲构象部分转变为反式构象,表明糖尿病人血清中蛋白质的有序结构发生变化;类脂物特征峰1 449 cm-1相对强度增加了58%,与糖类有关的特征峰谱线1 331,1 099和740 cm-1相对强度分别增加了35%,100%和62%,从而表明糖尿病人血清中与脂类和糖蛋白有关的物质含量增加。

Thus gp130 is the common signal transduction molecule. Gp130 molecule expresses universally on many tissues including immune system, hemapoietic system, and nerve system. Gp130 has an essential role in the function and regulation of the above systems, especially in the development of hemapoietic system. The signal transduced by gp130 is necessary during the differentiation and proliferation of hemapoietic cells. It is evidenced that the embryo of gp130 gene-knockout mouse was dead due to a serious impediment of the development of hemapoietic system.

Gp130在人体组织细胞上广泛性表达,介导和调节机体的免疫、造血以及神经系统的生理功能,尤其是在造血系统的发育过程中,具有极其重要的作用,动物实验表明,敲除小鼠的gp130分子基因可以使其在胚胎的早期阶段产生严重的造血系统障碍,最终导致流产,由此表明在造血细胞的分化、发育和生物学行为中,gp130介导的信号是必不可少的。

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上,并将之转入大肠杆菌HB101中,得到转纳豆激酶基因工程菌,提取重组质粒经单酶切、双酶切及PCR分析验证后,对重组菌的生长及表达进行一系列研究,结果表明:外源纳豆激酶基因的导入对宿主的生长没有明显的影响:重组质粒的稳定性实验表明:该质粒具有良好的分离稳定性,而结构稳定性较差。

Based on Hutchinson niche and Guangxia Cao\'s definition, Fuzzy hypervolume niche evolves the theory of fuzzy niche: the niche is a fuzzy hypervolume; the niche of the species is a hypervolume with fuzzy boundary which is formed by the n-dimensional resources (time, space, temperature, humid, trophic,...) necessary for the species\' continuous existing and multiplying; membership of a point in the niche is equivalent to the adaptive degree of the species to the point of the environmental resources.

在Hutchinson生态位和Guangxia Cao的定义的基础上,模糊超体积生态位发展了模糊生态位理论:物种的生态位是可以使物种生存和不断繁殖的n维环境变量(时间、空间、温度、湿度、营养……)所形成的边界模糊的一个n维超体积;隶属函数表明n维资源空间的一个点x属于生态位的程度,也表明物种对环境资源的适应程度。

The results show that the optimal value of CoCl2 concentration is 0.2 mol/L and the maximum adsorbed amount is 19.674 mg/g onto this adsorbent. The adsorption capacity of phosphine onto CoCl2-modified ACF decreases with the increase of temperatures. The maximum absorbed amounts are 19.674 mg/g at 298 K, 13.537 mg/g at 313 K and 11.087 mg/g at 328 K, respectively. It is found that the Freundlich equation is more suitable for the description of phosphine adsorption process than the Langmuir equation. The isosteric heat of adsorption decreases with the increase of the surface loading on CoCl2-modified ACF, which means that CoCl2-modified ACF adsorbent has an energetically heterogeneous surface. Meanwhile, adsorptive phosphine removal performance may be a dominant of physical adsorption when the heat of adsorption is 16-24 kJ/mol, the CoCl2-modified ACF adsorbent will be one of the candidates for tail gas purification of airtight calcium-carbide furnace and recycle of phosphine.

研究结果表明:浸渍液浓度最佳值为0.2 mol/L,此改性ACF对PH3的饱和吸附量为19.674 mg/g;PH3在CoCl2改性ACF上的吸附量随温度升高而迅速降低,在298,313和328 K时PH3的饱和吸附量分别为19.674,13.537和11.087 mg/g;Freundlich吸附等温方程较好地模拟了PH3在改性ACF上的等温吸附;PH3气体在改性ACF上的等量吸附热随吸附量的增大而减小,表明改性ACF吸附剂表面能量的不均匀性;吸附热在16~24 kJ/mol范围内,过程为物理吸附,有利于密闭电石炉尾气的净化。

By dehydrochlorination reaction the poly[(2-methoxyl-5-octyloxy)phenylene vinylene] is synthesized and the material"s structure is characterized by nuclear magnetic resonance spectroscopy, infrared absorption spectrum, ultraviolet and visible spectrophotometry. At the meantime, the fluorescence spectrum is measured and the mechanism of fluorescence occurring is analyzed. Thermal Gravimetric Analyzer"s results show that the MO-PPV"s thermal stability is fine. Also the experiment of resolvable property indicates that the MO-PPV can well dissolve in chloroform. Through analyzing the film"s surface by Metaloscope and Atomic Force Microscope, the good filming is found.

通过脱氯缩合法合成了聚(2-甲氧基-5-辛氧基)对苯乙炔,并分别用核磁共振波谱法、红外吸收光谱法、紫外-可见分光光度法等对其进行了结构表征;测量了样品的荧光光谱,分析了荧光产生的机理;热失重仪分析了材料的热稳定性,研究表明MO-PPV材料具有良好的热稳定性;溶解性实验发现材料在氯仿中溶解性较好;金相显微镜和原子力显微镜研究了薄膜的表面形貌,发现该材料的成膜性良好;材料的导电性研究表明,固体材料在掺杂下才可以表现出导电特性,而配成溶液的材料导电性因溶剂的不同而不同,并随溶解时间的长短发生变化。

E. , methine carbons were spit into the two components with inclusion of PVP to system. The above observation were responsible for the formation of intermolecular hydrogen bonding interactions. The proton spin-lattice relaxation time T〓, T〓 in the laboratory and the rotating frame were measure as a function of the blend composition. In view of the relaxation parameters, it is concluded that the Phenoxy and PVP chain are intimately mixed on the scale of 20-30A.

采用反转-回复法,我们测定该体系的T〓,T〓值的测定进一步支持,根据Tg判断的相容性。T〓ρ的研究结果表明,该体系在分子水平上是相容的,根据自旋扩散过程,估算该体系的相容性尺度为20-30〓,实验的1/T〓ρ组成关系同理论的线性关系比较呈负偏差,表明共混改变了组分在体系中的混合状态。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞,分析其生长分化特性,发现 1)突变型细胞可在未分化状态下维持长时间的增殖,表明EGFR对EG多能干细胞表型无明显影响;2)〓 M的维甲酸A(retinoid acid A,RA)诱导后,大部分对照组细胞分化为脂肪细胞,而突变型细胞分化为脂肪细胞的比例明显较少,表明EGFR在脂肪细胞的发育分化中具有一定的调节作用;3)畸胎瘤切片分析显示,突变型瘤组织分布有大量的未分化细胞和结缔组织,分化细胞以肌肉细胞为主;对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

The results show that all aluminum anodizing sludges were slightly neutral to acidic and mainly consist of more than 50% Al2O3; the similar total contents of Cu, Pb, Zn, Ni, Cd, and Cr in the 7 samples were found, namely, the concentrations of Pb, Cd were relatively lower, but that of Cu, Zn, Ni, and Cr were higher; the leaching tests indicate that all the samples were classed as nonhazardous wastes; but each of metals in the sample shows very different leaching behavior, possibly depending on their total contents and the final pH of leachates.

结果表明,铝型材污泥属于中性偏酸性物质,主要由Al2O3组成,含量在50%以上。各试样中Cu、Pb、Zn、Ni、Cd及Cr的总含量的分布趋势基本是一致的,其中Pb、Cd的含量较低,Cu、Zn、Ni及 Cr的含量较高。浸出毒性试验表明铝型材污泥属于非危险废物,但污泥中各重金属的浸出特性又各自不同,可能主要与它们在试样中的总含量和浸出液的最终pH值有关。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白,后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾,编码RNA聚合酶,含有微小RNA病毒RNA聚合酶的八个保守基序,同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。