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Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明:低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠,促使种子发芽:在不添加任何植物激素的培养基中诱导刺五加体胚形成,其中,1/3MS+3%蔗糖的培养基搭配有利于液体培养的刺五加体胚增值,1/3MS+1.0%蔗糖的培养基搭配有利于固体培养的刺五加体胚增值;在进行体细胞胚增殖时,体细胞胚继代三周后表面开始形成很多次生胚,对刺五加次生胚进行细胞学观察,发现刺五加次生胚与母体只是粘贴在一起;刺五加体胚需在液体培养基中培养一段时间形成幼植物体,实验表明可以通过生物反应器培养大量的刺五加幼植物体,然后转入固体培养基中形成完整植株,因此可以通过生物反应器培养进行规模化生产。

The results indicated that the treatment of irradiation restrained the growth and differentiation of protocorm, especially to roots.The LD50 is 67.23Gy, because the protocorm is a integrate embryoids and higher tolerance was found. Ploidy analysis showed that most of aberrant seedlings go with the change of DNA and they were not euploid. When the plants trearted by irradiation, some of aberrant seedlings appeared stem forfication, and leaves appeared chlorophyll-deficient or green-white.,probably due to some genes' expression or closing.

结果表明:辐照处理对种胚原球茎的生长和分化有抑制作用,尤其是对根的抑制更为明显;由于种胚原球茎的完整性好,耐受性强,其辐照半致死剂量(LD50)为67.23Gy;流式细胞分析表明,大多数外部形态发生改变的植株其细胞内DNA的倍性发生了改变,出现相当多的非整倍体;此外,辐照产生的变异苗出现茎分叉、叶片缺绿或白绿相间等现象,可能是由于某些基因的表达与关闭引起的。

The interior sound pressure levels and exterior sound pressure levels of the complex box structure excited by an exterior point load are measured, and a good agreement between the theoretical results and the test results shows that the theoretical method and corresponding results are correct.

通过对几种具有理论解的结构辐射声场的分析,验证了本文理论的正确性,研究表明本文方法具有很高的精度;同时对复杂箱形结构进行了实验验证,在实验室条件下测量了复杂箱形结构在外部点载荷激励下的内、外部辐射声压级,与理论预估值比较表明,二者具有良好的一致性,进一步证实本文所建立的理论预估方法是有效的。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果,对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾,经分析得出如下结论:1、本研究骨质疏松模型采用单一人工干预方法,没有其他影响因素;实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成,同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响;因此,同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中,模型组与对照组研究结果的差异表明:铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下,形成了一个互相影响的假设图:铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Cyanide in water, in tissues of aerial part of plants and in air was analyzed spectrophotometrically. Results from this study indicated less than 10.85% reduction of the applied iron cyanide complex was detected in hydroponic solution in the presence of plants. Little amounts of cyanide were found in all parts of plant tissues, indicating the passage of ferrocyanide through the plants.

结果表明:(1)低浓度实验组52.99mg CN L^(-1水溶液中10.85%的亚铁氰化物被植物吸收,随着浓度的升高吸收到植物体内的亚铁氰化物的比例依次递减,但是统训学分析显示各实验组单位体重的植物吸收亚铁氰化物的量无显著性差异;(2)在植物的各个部位都能检测到微量的亚铁氰化物,表明亚铁氰化物通过植物的蒸腾作用在植物体内的迁移。

Little amounts of cyanide were found in all parts of plant tissues, indicating the passage of ferrocyanide through the plants.Mass balance studies showed that iron cyanide complex moving into plants from hydroponic solution can be metabolized during transport.

由于没有检测到在气态下的总氰化物,表明植物的蒸腾作用没有将亚铁氰化物释放到大气中;(3)尽管植物吸收到体内的亚铁氰化物足有限的,但物质平衡实验表明其在植物体内迁移的过程中超过96%的郜能被植物有效转化;(4)所用的4种亚铁氰化物浓度在192 h内没有对柳树产生毒性作用。

To evaluate the bioactivity of the glass-ceramics,the specimens were soaked in the simulated body fluid. The results indicate that fluorapatite shows needle-like microstructure, which is similar to that in human bone and teeth. The formation of needle-like fluorapatite is related to the heat treatment procedure, which results in needle-like fluorapatite crystals by accelerating one-dimension growth along the c-axis through the screw dislocation growth mechanism. The mechanical properties of the glass-ceramics are close to those of the human bone and teeth. The bioactivity test results show that apatite layer is precipitated on the materials surface after 3 d immersion in SBF, which indicates the glass-ceramics have a good bioactivity.

研究结果表明:氟磷灰石相呈针状,与人体骨和牙齿的磷灰石形貌相似,有利于提高材料的生物活性和生物相容性;氟磷灰石的形成是材料被迅速加热至高温进行热处理,使磷灰石以螺旋位错生长机制沿c轴晶化长大所致;经热处理获得的材料具有优良的力学性能,基本满足骨科和齿科用生物材料的性能要求;材料在体外模拟体液中浸泡3 d后,样品表面已有磷灰石层生成,表明该材料具有较高的生物活性。

In the device, a four-post frame was adopted, two synchronously rotating grip holders were used to realize rigidity rotation of the specimen, and the axial movement of the upper grip holder implemented the tension/compression loading. Positioning accuracy in the axis, radial and circumferential directions were achieved by adopting many advanced and delicate technologies. Measurement on the device indicated that the actual positioning accuracy of the device was better than the kinematic positioning accuracy. Evaluation test on the system indicated that the spatial resolution is better than the pixel size without considering the noise.

该装置采用了四柱式框架结构,采用上下夹头同步转动实现试件相对于框架的刚性转动;上夹头作相对于框架的轴向运动实现对试件的加载;在加载和转动装置中采用了诸多高精技术,实现了试件高精度的轴向定位、径向定位和刚性角度定位;对装置的精度检测表明其实际的定位精度优于运动学定位精度要求;对系统的计量考评初步表明,系统不计噪声的空间分辨率优于X射线CCD的像素10.9μm。

The two Fraunhofer lines at 688nm and 760nm are obvious in the radiance spectra by ASD FieldSpec Pro NIR spectrometer,which largely overlap the chlorophyll fluorescence emission spectrum of leaves.

第三,研究并分析了夫琅和费暗线方法计算的688nm和760nm波段的荧光特性,结果表明该方法计算的荧光是可靠的,它与光合有效辐射关系密切,复相关系数达到了0.9;冬小麦冠层荧光光谱在760nm和688nm波段的荧光大小基本相等,而地锦冠层荧光光谱在688nm波段的荧光强度是760nm的3倍左右,表明荧光光谱能够更加敏感地反映植被物种或生理生化状况的差别。

The tests have Simulated the rainstorm state and the order state, and the results indicated the disposal of microorganisms-aquatic plant system is efficient. The optimization removal rate time was ascertained under the static state test, in dynamic test, comparing and analyzing the effect on removal rates of different concentration wastewater. The results showed, without aeration, the removal rate of CODCr, TN was 85.9%,75.1%, respectively, the highest removal rate of TP was 64.6%, and the concentration of DO increased steadily from 2mg/L to 7mg/L during experiment. In dynamic test, the microorganisms-aquatic plant systems have also appeared better effect on freshet, and the removal rate of TN,TP and CODCr, was 64%,69% and 81%, respectively.

利用接种高效微生物菌剂促进填料快速挂膜,并和水生植物联合作用去除水体中的氮磷等污染物,在静态水体下研究达到最佳去除效率的时间,并在连续流试验下对比分析不同浓度下的去除效率,根据静态模拟试验结果表明,在不曝气,自然复氧条件下,CODCr和TN的最终去除率分别为84.3%和71.4%,TP最高去除率为62.9%,DO也从2mg/L稳定的升高到7mg/L左右;在连续流模拟试验结果表明,该系统对雨后入湖的河水中TN、TP及CODcr的去除率分别稳定在近64%,69%和81%以上。

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