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Results showed that hypochromism and red shifted KAE absorption spectra were observed in the presence of DNA,and that synchronous fluorescence of DNA-NR was quenched with addition of KAE,indicating that a strong competition for DNA binding ...

结果表明:加入DNA后,山柰酚的吸收光谱呈现减色和红移现象;山柰酚的加入使得DNA-NR同步荧光强度猝灭,表明山柰酚对NR与DNA的结合为竞争性抑制;山柰酚的加入使得DNA的粘度增大,热变性温度升高。

Results showed that hypochromism and red, shifted KAE absorption spectra were observed in the presence of DNA, and that synchronous、 fluorescence of DNA-NR was quenched with addition of KAE, indicating that a strong competition for DNA binding between KAE and NR existed, in addition, the relative viscosity and the value of melting temperature of DNA increased in the presence of KAE.

结果表明:加入DNA后,山柰酚的吸收光谱呈现减色和红移现象;山柰酚的加入使得DNA-NR同步荧光强度猝灭,表明山票酚对NR与DNA的结合为竞争性抑制;山柰酚的加入使得DNA的粘度增大,热变性温度升高。

Multiple-paths hydrogen abstraction reactions of fluorine -with CH_3OH have been investigated at the MP2/6-311 ++ g level. The potential energy surfaces for H-abstraction reaction from carbon atom and oxygen atom were given respectively. The results indicate that there is only one minimum energy path for hydrogen abstraction from carbon atom, while multiple paths were found for hydrogen abstraction from oxygen atom. All the stationary structures, frequencies as well as PES sketch of all channels were given, in which the dominant channel is by a five-member ring transition state. The calculated heat of reaction is 170.62 kj/mol for channel 1, 119.41 kJ/ mol for branch channel 6. The results are in good agreement with experimental data.

以MP2/6-311 ++g水平上详细研究了氟原子与甲醇抽氢反应的多通道反应机理,得到了各条通道中涉及的驻点的构型和振动频率及其能量,给出了两张完整的反应势能面,结果表明,氟原子从C原子上抽氢时有一条明显的最低能量通道,而从氧原子上抽氢时要涉及多条分支通道和多个驻点构型,给出了各分支通道势能面示意图,结果表明以形成五元环状过渡态通道为优势通道,计算得到经途径1生成CH_2OH时反应放热170.62kJ/mol,经分支途径6生成CH_3O自由基时反应放热119.4 kJ/mol,此结果与实验值一致。

UV spectra of the n -octanol gels formed by the three gelators indicate that the K bands of benzene rings are red shifted. It reveals that the π-π stack is one of the driving forces for the self-assembly of the gelators. The extent of red shifts of K bands depends on the amount of methyl groups in benzene rings which is in accord with the stabilities of the gels formed by such three gelators.

紫外吸收光谱表明,对比其溶液态,三种衍生物聚集体苯环的K带发生红移,表明π-π堆积作用是亚苄基山梨醇衍生物凝胶剂自组装的驱动力之一;红移的幅度随苯环上甲基数量的增加而增加,这与三种衍生物形成的分子凝胶的热稳定性相吻合。

The results showed that mild alkali stress (pH 6.0~6.5) promoted plant growth, while severe high pH inhibited both seed germination, photosynthesis and root elongation.

结果表明:pH值过高会抑制种子萌发;随着pH值的逐渐增加,根的相对生长量和幼苗叶绿素含量均呈现先增加后减少的趋势;同时,根尖生长素含量随着pH值升高呈现先增加后减少的趋势,表明生长素含量变化可能在植物对碱胁迫的响应中,尤其是在根伸长过程中起着重要作用。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体,然后导入毕赤酵母(P8chia nastoris)菌株GSlls细胞中,在甲醇的诱导下,经过酵母高密度发酵进行PAP的表达,经SDS-PAGE分析,结果表明,在培养基上清液中含有一明显的特异性蛋臼条带,大小为34Ku,经Western-blotting分析,该蛋白与法国PAP抗血清有特异性反应,体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性,说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

The complexes areneutral as shown by values of molar conductivity in aqueous solution, namely,〓 The crystal structure determinations show that, in complexes, metal ions werecoordinated by four nitrogen atoms and form slightly distorted square planar configuration,relationship between fluorene ring plane and coordination plane is similar to those betweenpeptide planes of β-folding in proteins. The complex molecules linked by hydrogen bonds formone dimension chain or two dimension network structure.

摩尔电导测定表明他们都是二氧四胺中酰胺氮去质子化后形成的产物。X-射线衍射晶体结构测定表明〓的铜、镍配合物的配位多面体都是稍微畸变的平面正方形,配合物分子中芴基平面与配位平面的关系类似于蛋白质β-折叠结构中肽平面之间的关系,配合物分子之间通过氢键连接成一维链状或二维网状结构。

He results show that the virus regained infectivity, and the tannins did not direct viricidal effect. Tannins extracted from Polygonum perfoliatum or Archontophoenix alexandrae seeds showed strongly anti-TMV activities separately. TMV virions seems to be aggregated when TMV incubated with the tannins by electron microscope. It seems that TMV coat protein bind to the tannins based on PAGE. Tannins did not reduce infectivity when applied either before or after TMV inoculation. It is proposed that tannins extracted from the 11 species inhibited TMV by binding with TMV coat protein to interfere with recognition site essential for viral coat protein. Tannins extracted from Euphorbia hirta, P. perfoliatum, P. cuspidatum reduce infectivity when applied immediately in N.

MV分别与这11种植物单宁混合后,通过透析除去单宁后,可恢复大部分侵染力,表明单宁对病毒粒体并无直接毒害作用;TMV与杠板归和假槟榔种子中提取的单宁分别混合后,电镜下观察粒体呈现明显的聚集现象;TMV外壳蛋白分别与这两种植物单宁混合后,聚丙烯酰胺凝胶电泳中CP泳带滞后;单宁在病毒接种前施用未降低TMV对叶片的侵染能力;这些结果表明植物单宁对TMV抑制作用可能是单宁与TMV-CP结合影响病毒对叶片侵染位点的识别而降低TMV侵染性,而这种结合是可逆的。

"Our findings are consistent with earlier studies that have shown a high degree of association between depression and disability," commented lead author Saba Moussavi of the WHO and colleagues.

经过计算,衡量基本生活质量的&全球平均健康指数&表明,沮丧是让人最难忍受的。研究人员称,&此前的研究表明,沮丧与健康不佳之间有着很高的关联性,我们的研究也与此一致。&

The results show that when gravity wave's propagating in the homogenous winds, the nonlinearity is stronger in the fair wind than in the contrary wind, the background winds change the propagation velocity and path, and when gravity wave's propagating in the homogenous winds, the vertical wavelength decreases, the average upgoing energy flux reduces which implies the ambient winds absorb the energy of the wave, realizes coupling of the different atmosphere layers.

结果表明,当重力波在均匀风场中传播时,非线性效应在顺风场中比在逆风场中更显著,背景风会改变重力波在水平方向的传播速度和传播路径;当重力波在剪切风场中传播时,重力波在垂直方向上平均能流随高度的增高而减少,表明背景吸收了重力波的能量,在重力波向上传播的过程中,实现了大气各层之间的耦合。

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