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A full-length cDNA encoding sucrose: sucrose 1-fructosyltransferase from Lactuca sativa was inserted into pCAMBIA1300-als under the control of the CaMV 35S promoter. This plasmid was used for Agrobacteriummediated tobacco leaf disc transformation. Transgenic plants were analyzed by PCR and Southern blotting. 1-SST gene expression was confirmed by RT-PCR.
将从莴苣中克隆的1-SST基因重组到pCAMBIA1300-als中,构建了在CaMV 35S启动子调控下的植物表达载体,利用农杆菌介导的叶盘转化法将1-SST基因导入烟草中,PCR和Southern杂交检测表明获得了转基因植株,RT-PCR结果表明该基因在烟草中正常表达。
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Geochemical analyses show that the melange blocks within the Altyn ophiolitic melange zone have different characteristics from various tectonic environments.
地球化学分析表明阿尔金蛇绿混杂带中的混杂岩块具有不同的地球化学特征,表明其来自于不同的构造环境。
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Oral tapers of less than three weeks duration show a higher chance of relapsing [28][29] , and tend to show poorer outcomes .
口腔蜡烛的不到3周的时间表明,高复发的机会[ 28 ] [ 29 ] ,而且往往结果表明穷。
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Results showed that the specific activity of nitrile hydratase per dry cell weight in engineered Rhodococcus strains driven by Ptac, Pami-1, Pami-2 and PlacZ was 7.5, 6.3, 5.3 and 1.8 times of that in the wild, respectively. It indicated that these promoters could be well recognized by RNA polymerase of Rhodococcus.
结果表明,启动子Ptac、Pami-1(7 bp SD-ATG间隔)、Pami-2(13 bp SD-ATG间隔)和PlacZ在紫红红球菌ATCC 33278中启动表达腈水合酶的比酶活分别是野生菌株的7.5、6.3、5.3和1.8倍,表明这些启动子都可以被红球菌的RNA聚合酶所成功识别。
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The physical maps of mtDNAs from N.taihuensis Chen,Salvelinus taxa and Salmo salar were analyzed.Genetic distances between mtDNAs were calculated by the formula of Nei and Li(1979).Phylogenetic reconstruction method,UPGMA,was used to analyze the genetic relationships and the molecular dendrogram was established and revealed:the genetic distance between Salvelinus taxa and Salmo salar is 12.23% and that between N.taihuensis Chen and Salvelinus taxa-S.salar is 21.36%.Thisc indicates that Salangidae diverged from Salmoninae earlier.
比较了鲑形目3种鱼的线粒体DNA物理图谱,根据限制性位点差异法计算它们之间的遗传距离,利用UPGMA聚类分析法构建分子聚类图,结果表明:红点鲑属与大西洋鲑先聚在一起,再与太湖新银鱼相聚,前两者间遗传距离为12.33%,亲缘关系较近,太湖新银鱼与这两者间的遗传距离为21.36%,亲缘关系较远,表明银鱼科与鲑科分离较早。
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Bioassaying of squash silverleaf assigned the whitefly from Beijing (host: cotton and sweetpotato) and Xinjiang to B biotype. However, populations from Fujian and Guangxi without silverleaf symptom were considered as non-B biotype.
银叶生测实验表明:北京甘薯、棉花和新疆茄子上的烟粉虱为害西葫芦后可引起寄主的银叶,表明属B型烟粉虱;福建甘薯和广西南瓜上的烟粉虱种群在西葫芦上为害不呈银叶属非B型。
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The sequence analysis indicated thatCHS, F3H, DFR and 3GT cDNAs encoded the polypeptide of 389, 358, 382 and 448 aminoacid residues, respectively. Sequences of the encoded polypeptide comparison showed thatthey shared 76-96%identities with each corresponding solanaceous proteins reportedpreviously.
序列分析表明,以上四个基因分别编码389、358、382和448个氨基酸残基的多肽,在氨基酸水平上与茄科植物相应多肽的同源性最高,达到76-96%;多重比较和系统发育分析表明它们分别属于各自基因家族中的一员。
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The comparison test of this new methodwith spray inoculation method was conducted. The results showed the former method could increase the incidence ofdisease and uniformity than the latter one. Used cotyledon-brushing with conidial suspension and fungicide foliarspraying method, the EC 50 values of hexaconazole, myclobutanil, triadimefon, thiophanate-methyl and chlorotha lonil to Sphaerotheca fuliginea treated in different time after inoculation were determined; the results showed that 96 h was better time for disease severity evaluating.
比较了白粉病菌分生孢子悬浮液涂抹法和喷雾法接种黄瓜幼苗子叶的效果,结果表明,涂抹法发病率高,均匀度更好;测定了接菌后不同时间施药,白粉病菌对己唑醇、腈菌唑、三唑酮、甲基硫菌灵和百菌清等 5 种杀菌剂的敏感性,结果表明,接菌后 96h 施药较为敏感,测得的 EC 50 较小。
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The average sequence divergence between individuals was 0.0006, while the range within a population and between populations were both 0-0.0013. Fst values were 0.0704, 0.0491, and 0.0792 for Cox1, ND1, and the combination of both genes. These data showed significant genetic variances (P.05) among geographic populations. The taimen populations in Heilongjiang River could be divided into four subpopulations based on Fst values between these geographic populations. However, these populations shared one common haplotype (BH11) shown by haplotype network and haplotype distribution frequency analysis. This suggested that they evolved from a common ancestor (likely a population in the upstream of the Heilongjiang river) and shared common gene flows.
单倍型网络和AMOVA分析结果均表明黑龙江流域哲罗鲑存在显著的群体分化,Cox1基因、ND1基因及组合数据的群体间Fst分别为0.0704、0.0491、0.0792,均达到显著性水平(P.05),根据配对群体间Fst可以将黑龙江流域哲罗鲑群体分为黑龙江上游群体、呼玛河群体、乌苏里江群体、内蒙古伊敏河上游群体4个地理群。9个群体共享同一个单倍型(BH11),表明这些群体具有相同的演化历史,为同一个祖先群体演化而来。
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In addition,during the investigation of cadmium different levels distribution in Typha angustifolia Linn,we find that:l as far as the accumulate ability of different organ in Typha angustifolia Linn are concerned,root sheath leaf.
此外对狭叶香蒲体内镉的化学形态分析表明根和叶中均以氯化钠提取态最高,但在根中以去离子水和乙醇提取态存在的镉含量总和大于氯化钠提取态,表明镉在根与叶的化学存在形态有所不同。
- 推荐网络例句
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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.
Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。
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I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.
我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。
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The shamrock is the symbol of Ireland and of St.
三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。