表明的

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The forest was characterized with phanerophyte evergreen plants with single-mesophyll-leathery leaves, which reflected the nature of tropical Karst mountainous seasonal rain forest.(2). Community succession was divided into five stages by using Cluster method, PCA method; the succesion stages include herbaceous stage, scrub-shrub stage, small tree stage, arbor stage and climax seasonal rain forest stage.(3). Based on similarity theory, succession sere was constructed with the community similarity equation formed.

结果表明：(1)森林群落在外貌上，以常绿高位芽、单叶、革质、中型叶植物为主，反映了热带岩溶山地季雨林的特点；(2)运用聚类分析、PCA排序等方法将群落演替阶段划分为草本群落阶段、灌丛阶段、小乔木阶段、乔林阶段、顶极季雨林阶段；(3)运用相似学原理，组建群落间相似度方程并以此方程构建了群落演替系列；(4)研究表明桂西南岩溶区生态系统健康受到严重胁迫。

Corbicula fluminea (Müller 1774) was bred in the laboratory and two shell samples (shell height, A=13 mm, B=9 mm) were selected to determine the carbon isotopic composition of the increment shells (aragonite, CaCO3) and the host water. The results indicate that the carbon isotopic compositions of the two shells increased as the δ^13 C values of the host water increased from -5.24‰ to 1.41‰, showing the influence of δ^13C values on the shell carbon isotopes.δ^13C value ranges from -4.76% to -2.09% and δ^13 C from -8.49% to -2.89%. The δ^13 C values of shells A and B are both more negative than the predicted equilibrium ones, indicating that the shell had used the metabolic CO2 rich in 12C during their growth process.

对双壳类河蚬进行了室内养殖，并选取两个壳体样品（壳高，A=13 mm， B=9 mm），测定其生长部分壳体和生长期间水体碳同位素，研究表明，随着水体碳同位素的升高（δ^13C由－5.24‰升至1.41‰），两壳体碳同位素也随之升高，表明水体对壳体碳同位素的影响；δ^13CA分布范围为－4.76‰~-2.09‰，δ^13CB为－8.49‰~-2.89‰，壳体A和B碳同位素均比预测平衡值偏负，表明壳体在形成过程中利用了新陈代谢产生的富集12C的CO2。

Canonical correlation analysis also indicated that forest community structure variables were correlated significantly with light factors, and both were interacted with each other and correlated well with PCNM variables.

典型相关分析结果表明群落结构因子和光因子相关性显著，两者都与PCNM变量极显著相关且相互影响，表明群落结构和光因子都表现出显著的空间结构。

The call developmental process of Psittacula agapornis consists of five instinct phases according to its call character. In the first phase （congenital cal1 phase, CCP, 0~12 day of age）, the juveniles only produce single syllable with short syllable length. The second phase（blank-model-forming phase,BMFP, 13~30 day of age） is important for formation of vocal learning pathways. During this phase, the calls characterized by more and longer syllables. In the third phase （call-model-forming phase,CMFP, 31~45 day of age）, the call development is guided by a model system for vocal learning, vocal learning is engendered so that some calls with multi-syllables with UP1 are produced, principal frequency has increased about by 920 cent, the number of note is double that of second phase. The short-term memories are transformed into long-term memories in the forth phase （call-feedback-learning phase,CFLP, 46~90 day of age）. The call of juveniles is a close match to tutor call which accompanied by double various sounds with BS and UP1, and more resonance components were formed. Second principal frequency has increased about by 970 cent, the number of note increases by 2.6~3.0 times. During the fifth phase （excellent call phase,ECP, after 91 d）, vocal motor pathway is developed and the calls are accompanied with most stable, harmonious vari-tone and complex repertoire.

牡丹鹦鹉的鸣声发育分为5个时期。0~12 d为先天性鸣声期，鸣声特征为以基本音为主音的单音节单音调声，声长短。13~30 d为空白模板形成期，鸣声特征为以基本音为主音的多音节单音调声，声长和音量显著性增加，表明发声学习通路开始形成。31~45 d为鸣声模板形成期，鸣声特征为以第1陪音（UP1）为主音的多音节单变调声，声长和音量显著性增加，主音频提升约920音分，涵盖的律音数增加1倍，表明发声学习模板逐渐形成，发声学习开始。46~90 d为鸣声反馈学习期，鸣声特征为以BS和UP1为主音的双变调声，声长和音量显著性增加，主音频涵盖的律音数增加2.6~3.0倍，第2主音频提升约970音分，表明稳定模板逐渐形成，短期记忆逐渐向长期记忆转化。91 d后为完美鸣声期，鸣声特征为以UP1为主音的复合变音调声，声长、音量、主音频的提升和涵盖的律音数都趋于平稳，鸣声稳定而和谐，表明发声运动通路基本形成。

In this thesis, drug distribution in local tissues after topical administration of the ATPs was investigated with HPLC technique, concentration of ACF in skin and muscle remained high during 12hours. Pharmacodynamic of ATPs was evaluated with diclofenac gel as a control, and it was found that ATPs had stronger anti-inflammatory activity than diclofenac gel.The results of rabbit skin irritation test and Guinea pig skin allergia test make it certain that ATPs is safe for topical administration.

本文利用局部组织活检法考查了大鼠局部应用ATPs后的局部组织分布情况，结果表明药物能在皮肤和肌肉组织中12h维持较高的药物浓度；将所制ATPs与市售双氯芬酸钾凝胶进行了药效学比较，结果表明ATPs的抗炎效果优于英太青；家兔皮肤刺激性试验结果表明，ATPs对完好皮肤无刺激性，对破损皮肤具有轻度刺激；豚鼠过敏性试验结果显示ATPs无致敏性。

The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明，石榴果皮提取物主要是通过抑制HMP途径，导致还原力NADPH缺乏，引起代谢功能障碍，达到抑菌的目的；加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明，石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂；SDS-聚丙烯酰胺凝胶电泳分析表明，石榴果皮提取物能够抑制菌细胞蛋白合成，尤其对菌体内大分子量蛋白作用最明显。

The drug release experiments in vitro indicated that with the increase of PLA molecular weight, the drug release rates decreased. 4ac releasing from 4ac-PLA-NP could be characterized by Weibull's equation. The interaction between 4ac and others compositions was not detected by DSC. The peak position of 4ac in DSC did not changed comparing 4ac with 4ac-PLA-NP lyophilize injection. Thc X-ray result showed that the peak of 4ac-PLA-NP was piled up between 4ac and other compositions.

差示扫描量热分析结果表明药物与各组分之间无明显的相互作用，制成4ac-PLA-NP冻干针剂后，其放热峰主要表现为聚乳酸的放热峰：X-射线衍射结果表明药物与载体间无相互作用，4ac-PLA-NP主要表现为4ac与载体之间的叠加峰；TLC、FT-IR也同样表明药物与载体无相互作用，它们之间是一种物理结合，并未有新物质生成。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。