表明

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

On the other hand, Large quantites of green pigment s are formed when garlic is pickled in vinegar or crushed into purees.

同时，在醋浸绿蒜中还存在大量的绿变色素，实践表明其对人体无害，且已有研究表明绿变色素的形成有活性物质的参与，因此可以考虑将其作为一种天然色素进行开发。

With the increased phosphorus supply, the net photosynthetic rate at saturated light, leaf area ratio, and the concentration of nitrogen and phosphorus increased in both species, and the biomass allocation of A. crenata to propagule also increased. So it was indicated that the increased phosphorus supply could facilitate the growth and reproduction of these two species.

结果表明：随磷营养水平的增加，两种植物在饱和光下的净光合速率和叶面积比升高，各器官的氮浓度和磷浓度均有所增加，其中朱砂根对繁殖器官的投入也增加，表明磷营养水平的增加能够促进其生长和繁殖。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

The phenetic characteristics including Gram stain, catalase test, the adaptation to different stress conditions and producing acid from carbohydrates were tested.

结果表明，分离菌株N7-03具有较强的苹果酸-乳酸酶活力[117.05mg苹果酸/log]，进一步的研究表明，N7-03具有良好的酿造学适应性。2。

We also use rank sum test to analyse the differe- nce between the light pre-eclampsia group and the severe pre-eclampsia group (P=0.000),the early onset pre-eclampsia group and the late onset pre-eclampsia group (P=0.003). All of the results give the same conclude that the differences within the three groups are significant (P.05). Conclusion: We conclude that HLA-G proteins are normally expressed in invasive trophoblasts and HLA-G proteins expression is defective in most pre-eclamptic placenta.We propose that trophoblasts lacking HLA-G proteins are vulnerable to attack by the maternal immune system.

结论HLA-G分子特异性分布于胎盘EVCT的胞膜及胞浆中，生理妊娠组、子痫前期组间差异性存在表明其表达量的下降与子痫前期的发病相关；轻、重度子痫前期组间显著差异表明HLA-G分子表达量的下降与子痫前期病情严重程度相关，早发型重度子痫前期患者胎盘组织中HLA-G分子的表达明显低于迟发型组，提示我们HLA-G分子表达的降低程度还与发病早晚有关。

The effects of Cd on oxidants and antioxidative enzymes in roots and leaves were investigated.

结果表明，随着Cd处理浓度的增加，叶片H_2O_2及叶片和根中MDA的积累量均较对照显著增加，表明Cd可诱导植株产生膜脂过氧化作用。

The illumination is of no effect on the germination rate of oospores of Phytophthora sojae.30 single-oospore isolates obtained from the test strain of Phytophthora sojae Ps-H2 (Race 1 ) were evaluated using hypocotyl-inoculation method for race phenotype on soybean cultivars. One clone phenotype ( race 1 )was selected randomly for further isolation and virulence identify .

将大豆疫霉菌供试菌株Ps-H_2(生理小种1号)每代挑取30个单卵孢株，进行生理小种鉴定，随机挑选1株1号生理小种的单卵孢株继续进行单卵孢株分离和毒性鉴定，连续两代试验结果表明大豆疫霉菌原始菌株并朱出现毒性分化，表明原始菌株即为1号生理小种的毒性纯系。

Experiment 1 showed that ＂yes-no＂ questions made 5-year-old children tend to answer ＂yes＂ in this task.

实验一表明，&是否&问题使得5岁儿童在该任务上表现出肯定回答倾向；实验二表明。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷